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Biomedical subjects

D Viza

Publications and source records attributed to D Viza.

At least 55 records · Page 3Linked to original sources
PubMed1978

Human lymphoblastoid cells in culture replicate immune information carried by xenogeneic RNA.

Immune RNA is obtained from lymphoid organs of immunized animals and is reputed to transfer immunological information. Human lymphoblastoid cells in culture, after incubation with sheep Immune RNA produce RNA (Ic-RNA) which carries the same immunological information as the inducing sheep preparation. This Ic-RNA produced in tissue culture is capable of converting 'naive' human lymphocytes to cytotoxic effector cells against tumour target cells, to the same extent as the Is-RNA preparation used for induction of the cell line. The sheep Immune RNA information is present and can be recovered from the lymphoblastoid cells for at least ten weeks after the induction. It is suggested that xenogeneic Immune RNA information is incorporated in a stable fashion by cultured human lymphoblastoid cells, and also that it is replicated during their own replication. This system could be used for studying the incorporation of information carried by exogenous RNA and it might provide insight into some mechanisms underlying the transfer and processing of immunological information.

Cells, Cultured↗
PubMed1977

[Replication of immune RNA by human lymphoblastoid cells].

Animal immune RNA used for the induction of the human lymphoblastoid cell line LDV/7, induces the appearance of receptor sites on the surface of these cells and the synthesis of specific immunoglobulins which are liberated into the culture medium. Furthermore, RNA has been extracted from these cells, which possesses the same properties of specific cytotoxicity as the RNA used for induction. It is supposed that the immune RNA derepresses specific genes on the genome of the induced cells.

Cell Line↗
PubMed1976

In vitro production of a transfer factor specific for transitional-cell carcinoma of the bladder.

Human dialysable Transfer Factor (TFd) extracted from lymphocytes of patients with transitional cell carcinoma of bladder (TCCB) was replicated in culture by lymphoblastoid cell lines. The effectiveness of two such TFdLs produced in vitro in transferring sensitivity to TCCB was assessed in the lymphocyte migration test (LMT) using formalin-treated TCCB cells as antigen. The results, showed that one TFdL transferred sensitivity in 5/14 cases and the other in 12/15, not only to leucocytes of healthy individuals but also to leucocytes of TCCB patients. Preliminary results showing an in vivo transfer of sensitivity are discussed.

Carcinoma, Transitional Cell↗
PubMed1975

Identification of an antigen associated with malignant melanoma.

Xeno-antisera, prepared by immunization with soluble membrane material obtained from melanoma tumours and partially purified by column chromatography, were found to detect an antigenic specificity common to some tumour extracts and sera of melanoma patients. The presence of this antigen in patients' sera allows speculation for its possible use for early diagnosis of metastases and its role in the blocking of the immunological defences of the melanoma patients. Preparation of monospecific antisera by immunization with insoluble serum-antigen/antibody complexes has been successfully undertaken.

Animals↗
PubMed1975

[Production of a dialysable transfer factor of cell mediated immunity by lymphoblastoid cells in continuous proliferation].

Four lymphoblastoid cell lines tested in this work contain normally a dialysable moiety having by ultraviolet spectroscopy, column chromatography (Biogel P 10) and chemically the same properties than human dialysable Transfer Factor (TFd), but unable to transfer cell mediated immune response against common antigens. Two of them are able to do so after incubation with minimal amounts of TFd. Production of a molecule identical to human TFd is possible in some lymphoblastoid cell lines after induction with TFd.

Animals↗