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Biomedical subjects

D Vecchio

Publications and source records attributed to D Vecchio.

26 records · Page 2Linked to original sources
PubMed1981

[DNA damage after treatment in-vivo with several aromatic amines: 2,4-diaminotoluene, 4-aminobenzene (aniline) and paradimethylaminoazobenzene (butter yellow)].

Three aromatic amines, 2,4 diaminotoluene, aminobenzene and paradimethylaminoazobenzene were examined for their capability of damaging rat liver DNA. Compounds were tested by the "in vivo"/DNA alcaline elution assay. 2,4 diaminotoluene was very positive, aminobenzene weakly positive, paradimethylaminoazobenzene probably negative. All the compounds were tested at doses already clearly toxic.

Aniline Compounds↗
PubMedDate not supplied

DNA damage induced by auramine O in liver, kidney, and bone marrow of rats and mice, and in a human cell line (alkaline elution assay and SCE induction).

Auramine O has been reported to be carcinogenic in rats and mice. It has been reported as positive in some mutagenicity studies and negative in others. We have found that commercial auramine O is positive in inducing DNA damage in vivo in liver, kidney, and bone marrow cells. DNA damage was also induced after treatment in vitro of a human cell line. Commercial auramine O was also clearly positive for sister chromatid exchange (SCE) induction in vivo in bone marrow cells. Purified auramine was negative in terms of DNA damage and SCE induction. Our commercial auramine O had Michler's ketone as a major contaminant. This compound was capable of inducing both DNA fragmentation and an increase of SCE.

Aniline Compounds↗
PubMedDate not supplied

Quantitative predictivity of the transformation in vitro assay compared with the Ames test.

For 59 chemical compounds, we have found homogeneous data on transformation in vitro, mutagenicity in the Ames test, and carcinogenicity. We have compared the potency in inducing transformation in vitro in hamster fibroblast cells with the carcinogenic potency and found a modest correlation coefficient between the two parameters (r = 0.37). For these same 59 compounds it was also possible to compare mutagenic potency in the Ames test with carcinogenic potency. The correlation level was very similar (r = 0.34). The predictivity of transformation in vitro increased significantly when only compounds for which some kind of dose-response relationship was available were utilized (r = 0.65). This result stresses the importance of the quantitative aspect of the response in predictivity studies. The present study is compared with previous studies on the quantitative predictivity of different short-term tests. Our work is not definitive, but gives an idea of the possible type of approach to the problem of comparing quantitative predictivities.

Animals↗