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Biomedical subjects

D V Coleman

Publications and source records attributed to D V Coleman.

At least 109 records · Page 6Linked to original sources
PubMed1978

Human polyomavirus (BK) infection and ureteric stenosis in renal allograft recipients.

Human polyomavirus (BK) was detected in two renal allograft recipients as a result of routine examination of Papanicolaou-stained smears of urinary sediment in the light microscope. Infection with this recently identified virus was confirmed by virus isolation and electron microscopy. The cytological, histological, and ultrastructural changes due to the virus are described, and virus excretion is correlated with the clinical progress of the patients and the pathological findings. The transplant ureters in both patients were found to be ulcerated and stenosed, and virus-infected cells were observed in the ureteric epithelium. We suggest that the administration of high-dose steroids in transplantation may permit active infection with human polyomavirus to occur in ureteric epithelium which has been damaged by ischaemia or inflammation.

Animals↗
PubMed1978

Partial trisomy 18 in a family with a translocation (18;21)(q21;q22).

A family is described in which 2 sibs had similar congenital abnormalities. Chromosome investigation of the mother and another child disclosed they were carriers of a translocation t(18;21)(q21;q22). The karyotype of one of the abnormal infants was determined and was found to be consistent with partial trisomy 18,46,XY,-21,+der (21),t(18;21) ((18pter leads to 18q21::21q22 leads to 2 lqter)mat.

Adolescent↗
PubMed1977

Human papovavirus in Papanicolaou smears of urinary sediment detected by transmission electron microscopy.

A technique is described whereby inclusion-bearing cells identified by light microscopy in stained smears of urinary sediment were reprocessed for examination in the electron microscope. The nuclei of the abnormal cells were found to contain numerous virus particles, 35 nm in diameter, which morphologically resembled papovaviruses. The technique was applied in this case to identify further the virus producing the cytopathic changes in the Papanicolaou smear. It could be particularly valuable for retrospective studies of mounted cytological or histological material when suitable specimens are no longer available for virological investigation.

Humans↗
PubMed1973

Human polyomavirus infection in renal allograft recipients.

Cytological and virological studies on 74 patients with functioning renal allografts were undertaken to detect polyomavirus infection of the renal tract. Ten patients (13.5%) were excreting polyomavirus. Virus particles were seen in the electron microscope in urine samples from eight patients. B.K. polyomavirus was isolated from four patients. Infection with a different polyomavirus was probable in one patient. Virus isolation was most readily achieved when large numbers of intact virus particles were seen in the urine. Patients who were excreting large amounts of polyomavirus shed numerous inclusion-bearing cells which could be detected by cytology. A serological study showed that the prevalence of B.K. antibody was similar to that found in the general population and 38% of this series of transplant patients had evidence of active infection with B.K. virus.

Adolescent↗
PubMedDate not supplied

Chromosome analysis of first trimester chorionic villus biopsies prepared by a maceration technique.

A technique for the culture of chorionic villi for cytogenetic studies is described which is simple, reliable, rapid, and suitable for routine laboratory use. This method eliminates the need for multi-stage and time-consuming procedures inherent in previous techniques which have been developed for this tissue. Cultures suitable for analysis were obtained in an average of sixteen days and the risk of maternal cell contamination was evaluated. Results of cultures initiated from 50 chorion biopsies obtained by transcervical blind aspiration at a weekly termination clinic are presented, and the potential use of this technique as a routine procedure is discussed.

Chorionic Villi↗
PubMedDate not supplied

Chorionic villus culture for first trimester diagnosis of chromosome defects: evaluation by two London centres.

The maceration technique for the culture of chorionic villi has been applied by St Mary's Hospital and King's College Hospital, to a total of 225 villus aspirates of which 100 were from diagnostic cases. Two hundred and eighteen (96.8 per cent) of these were successfully karyotyped, and chromosome abnormalities were detected in 15 cases. Of the cases with a normal karyotype, 113 were male and 90 were female. Sixty-nine (77 per cent) of the female cultures were demonstrated to be fetal and a further two of these may be verified at delivery. The use of Chang medium was found to accelerate cell growth thereby reducing the interval between sampling and reporting to less than 2 weeks. An unlimited quantity of metaphase spreads was obtained suitable for the application of routine banding techniques, and comparable to those obtained from amniotic fluid culture. Experience with diagnostic cases reinforced our view that culture should always back up direct analysis and this is discussed with particular reference to the occurrence of mosaicism in the trophoblast.

Chorionic Villi↗