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Biomedical subjects

D Turck

Publications and source records attributed to D Turck.

At least 127 records · Page 7Linked to original sources
PubMed1995

Importance in diagnosis of gastritis of detection by PCR of the cagA gene in Helicobacter pylori strains isolated from children.

The cagA gene has been detected by PCR and DNA hybridization in 45 Helicobacter pylori strains isolated from children. For each child, clinical symptoms, endoscopic aspect of the gastric mucosa, and histological gastritis were evaluated. Gene-positive strains were associated with hemorrhagic gastritis in 66.6% of the children, while gene-negative strains were associated with hemorrhagic gastritis in 11.2% of the children (P = 0.0001). In addition, 88.8% of gene-positive strains were associated with severe histological gastritis (scores of 3 and 4), and gene-negative strains were collected from the gastric mucosa with the same type of infiltration of neutrophils and lymphocytes in the lamina propia in 55.5% of the children. These differences were statistically significant (P = 0.017). Gene-positive strains were also isolated more frequently from children with vomiting (P = 0.04), while the absence of clinical signs was not significantly different in cagA gene-positive or -negative patients. All of these observations confirmed the role of this cagA gene as a marker of gastric inflammation in children. The detection of this gene might be helpful to determine the degree of inflammation of the gastric mucosa in the absence of abdominal symptoms. We might better understand the natural history of H. pylori infection if we studied the evolution of gastritis in children with regard to the cagA status of isolated strains.

Adolescent↗
PubMed1995

[Bacteriological monitoring of the treatment of Pseudomonas aeruginosa infections with amikacin administrated at once-daily dosis in patients with cystic fibrosis].

The interest of the treatment with a single daily dose of amikacin (AMK) in cystic fibrosis (CF) patients with P. aeruginosa infections has been much debated. The aim of work was to study the efficiency of this treatment on (CF) patients with chronic bronchopulmonary P. aeruginosa infections previously treated for two weeks with the combination ceftazidime (CAZ 200 mg/day in 3 inj. IVD) and AMK (35 mg/day in one IV perf. of 30 minutes). The bacteriological supervision of this treatment was performed 1: by the determination of MICs before and after treatment, 2: by the decrease of P. aeruginosa colonization immediately after this treatment and during 11 months, 3: by the identification of P. aeruginosa strains with phenotypic methods (serotyping and antibiotyping) and with genotypic method (pulsed field gel electrophoresis). The use of AMK in a single daily dose in order to treat chronic lung infections colonized with P. aeruginosa susceptible to this antibiotic shows encouraging results as far as bacteriology is concerned: this treatment has given means to reduce colonization for a month in 15 of 18 patients. For 9 of the 18 patients, no P. aeruginosa strains were isolated for nine months. The serotyping and antibiotyping systems do not enable us to study the P. aeruginosa epidemiology. Genome macrorestriction fingerprinting of P. aeruginosa in pulsed field gel electrophoresis confirms that patient with CF were colonized with one or several clones. In our study no variation of these clones was noticed for the first eleven months. Genome macrorestriction fingerprinting appears to be one of the most effective methods for delineate strains of P. aeruginosa colonizing CF patients.

Adolescent↗
PubMed1994

Prognostic value of esophageal manometry in antireflux surgery in childhood.

To assess the predictive value of preoperative esophageal manometric study in the outcome of antireflux surgery, 14 children with severe gastroesophageal reflux (GER) who underwent surgery were studied retrospectively. Five patients had neuromuscular disease; one had been operated on for esophageal atresia. After extended (> 20 h) esophageal pH monitoring and/or barium swallow study, all patients underwent preoperative manometric study. After surgery, the patients were followed for 4 months to 4 years. Functional complications were noted after mechanical complications were eliminated. All patients had normal upper esophageal sphincter pressure (UESp); the resting lower esophageal sphincter pressure (LESp) was decreased in four patients, and seven had esophageal body motility trouble. Functional complications occurred in two patients. One was a neurologically involved patient who had had a normal preoperative manometric study; the other was the patient who had been operated on for esophageal atresia. No complications occurred in four patients who had had abnormal preoperative manometric studies. We conclude that, in this group of patients, esophageal manometric study has no predictive value in the outcome of the surgical procedure; however, it still would be interesting to elucidate the mechanisms of GER, especially in congenital abnormalities such as esophageal atresia.

Child↗
PubMed1994

High prevalence of Helicobacter pylori infection in cohabiting children. Epidemiology of a cluster, with special emphasis on molecular typing.

Intrafamilial cases of infection with the same strain of Helicobacter pylori (H pylori) have been reported but these clusters were too small to distinguish between person to person spread or coinfection from a common environmental source. To gain more information on the mode of transmission of H pylori, an epidemiological survey with bacterial strain differentiation by restriction endonuclease analysis of chromosomal DNA was carried out in an institution of 117 children with encephalopathy (aged 3.5 to 19 years). All children with antibodies against H pylori had gastroscopy to obtain gastric biopsy specimens. The prevalence of infection (confirmed histologically or microbiologically, or both) was 38% (45/117), and rose to 67% in one of the five sections of the institution. H pylori was isolated in 34/45 cases, and 22 different strains were found of which five strains were present in more than one child. Up to seven children were infected by the same strain, five of them were living in the same section. Analysis of the characteristics of infected children showed the predominant role of living conditions and the period of time cohabiting in this unexpectedly high prevalence of H pylori infection in children living in good sanitary conditions.

Adolescent↗
PubMed1993

Detection of H. pylori in saliva using a monoclonal antibody.

The presence of Helicobacter pylori in saliva specimens collected from infected children examined before and after specific therapy and from a non-infected pediatric population was detected by indirect immunofluorescence assay (IIF) using a specific monoclonal antibody. Among the 25 children with H. pylori-negative antral biopsies, 4 had in their salivas bacterial cells similar to H. pylori, but a retrospectively performed serologic test showed a positive response. No false-positive reaction was observed among non-infected and seronegative children. The observation of bacterial cells with H. pylori morphology in saliva by IIF was consistent with the presence of this bacterium in antral biopsies. A person-to-person transmission of H. pylori by saliva thus appeared to be possible.

Adolescent↗
PubMed1993

Age and diet affect the composition of porcine colonic mucins.

Colonic mucins may serve as a defense mechanism by binding bacterial, viral, or dietary lectins, thereby preventing them from attaching to the intestinal epithelium. Presumably, the composition of the mucins would be responsible for this phenomenon, and the composition of mucins from mature mammals would be the most effective in binding lectins. To determine whether differences in diet and/or age affect the composition of colonic mucins, we scraped fresh colonic mucosae from pigs at 0 (n = 3), 7 (n = 3), 21 (n = 3), and 180 (n = 3) d of age and purified the mucins from these mucosal scrapings. Mucins were purified by ribonuclease and deoxyribonuclease digestion, high-performance size-exclusion chromatography, and cesium chloride density-gradient ultracentrifugation. The 180-d-old pig was considered mature. No changes were observed in any of the variables analyzed in the 7-d-old animals. No changes were observed in quantities of galactosamine and galactose. The amounts of fucose and glucosamine increased by 165 and 37%, respectively, (p < 0.05) from d 0 to d 21 in the sow-fed animals, at which time fucose and glucosamine content were 48 and 22% greater, respectively, than in the 21-d-old, artificially fed group (p < 0.05). A further significant increase in fucose content was observed in the mucins from mature animals. The sulfate content in the 21-d-old, sow-fed animals was significantly lower than in both the newborn and the 21-d-old artificially fed animals. The sulfate content in all three of these groups, however, was significantly higher than that observed in the mucins of mature animals.(ABSTRACT TRUNCATED AT 250 WORDS)

Age Factors↗
PubMed1993

[Cow's milk proteins intolerance disclosed by ulcero-necrotizing enterocolitis in a full-term infant].

BACKGROUND: Necrotizing enterocolitis associated with milk protein intolerance is rare. CASE REPORT: A girl, born at term, weighing 3,150 g, was fed several different formulas because of persistent vomiting and diarrhea; some of these formulas contained cow's milk proteins. At 5 weeks of age, the patient developed acute abdominal distension and obstructive manifestations. Laparotomy showed intestinal distension and perforation of the distal small bowel, requiring resection with temporary ileostomy. Histological examination of the resected segment of the small bowel showed extensive necrosis of the mucosa and submucosa with involvement of the muscular layers. The patient was given parenteral nutrition for 3 weeks then refed with human milk. Cow's milk was introduced at the age of 2 1/2 months; this was immediately followed by vomiting and an anaphylactic reaction, with increased ileostomy fluid volume and blood and sugars in stools. A jejunal biopsy performed 3 weeks later showed moderate villous atrophy with a dense infiltrate of eosinophils below the epithelium. The RAST test was positive to beta-lactoglobulin and negative to casein and lactalbumin. The patient tolerated cow milk by the age of 18 months. CONCLUSIONS: Cow's milk protein tolerance should be evaluated when necrotizing enterocolitis occurs in the absence of classical risk factors.

Animals↗