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Biomedical subjects

D T Williams

Publications and source records attributed to D T Williams.

At least 73 records · Page 4Linked to original sources

Duration thresholds for chromatic stimuli.

The duration necessary to detect chromatic stimuli was measured for wavelengths between 463 and 620 nm. Stimuli were presented either in hue substitution (replacement of white by a chromatic stimulus of matched luminance) or as increments. Two observers viewed a 1 degrees 45' homogeneous white field. A trial consisted of replacement of the central 40' of the field by a chromatic stimulus. In substitution mode the white field was 2.4 cd/m2., the chromatic replacement was of matched luminance using heterochromatic flicker photometry (HFP). In increment mode, the white field was decreased to 1.2 cd/m2., the chromatic replacement remained at 2.4 cd/m2. In substitution mode, duration threshold varied from approximately 3--4 ms for the spectral extremes to 45--66 ms at 570 nm. Detected stimuli were always seen as a change in chromaticity. In increment mode, thresholds were in the 2--4 ms range with no dependence upon spectral composition. Detected stimuli were seen either as changes in chromaticity or brightness. A control experiment indicated that HFP did establish equivalent luminance for the hue substitution mode. We conclude that duration thresholds in substitution mode reveal chromatic processing channels; duration thresholds in increment mode are mediated by chromatic and/or achromatic processing channels.

Color Perception↗

Isolation and concentration of organophosphorus pesticides from drinking water at the ng/L level, using macroreticular resin.

A screening method has been developed for determining organophosphorus pesticides at ng/L levels in drinking water. Sixteen organophosphorus pesticides, diazinon, diazinon-oxon, dimethoate, ronnel, beta-phosphamidon, methyl parathion, ethyl parathion, malathion, chlorpyrifos, fenitrothion, ruelene, methidathion, ethion, EPN, phosalone, and phosmet, were extracted by Amberlite XAD-2 resin from 100 and 200 L drinking water previously spiked with these pesticides. The pesticides were eluted from the XAD-2 resin with acetone-hexane (15+85). The concentrated extract was analyzed by gas chromatography using a nitrogen-phosphorus selective detector and by gas chromatography-mass spectrometry using selected ion monitoring. Recoveries at the 10 and 100 ng/L spiking levels were greater than 90%, except recoveries for dimethoate and phosphamidon were 37 and 42%, respectively. The analysis of 300 L Ottawa tap water showed no detectable amounts (less than 1 ng/L) of any of the 16 organophosphorus pesticides.

Chromatography, Gas↗

Neurogenic and hysterical seizures in children and adolescents: differential diagnostic and therapeutic considerations.

For patients whose seizures are not controlled by medication alone, psychotherapeutic intervention can sometimes play a useful adjunctive role in both differential diagnosis and treatment. Diagnostic issues include the differentiation between neurogenic and hysterical seizures as well as the delineation of the possible role of psychogenic stress in triggering neurogenic seizures. Therapeutic considerations focus on the alleviation of precipitating stresses as well as the development of a sense of mastery in the patient.

Adolescent↗

Organochlorine pesticide levels in Ottawa drinking water, 1976.

Duplicate samples of Ottawa drinking water were collected once a month during 1976 and analyzed for organochlorine pesticides. The samples were analyzed by gas chromatography--mass spectrometry, and pesticides were identified by comparing their retention times, coupled with selected ion monitoring, with those of known standards. The pesticides detected and their mean concentrations in parts per trillion were aldrin (0.9), heptachlor epoxide (3), heptachlor (0.6), alpha-BHC (6), gamma-BHC (3), endrin (4), dieldrin (1), o,p'-TDE (1), o.p'-DDT (3), and o,p'-DDE (0.2).

Hydrocarbons, Chlorinated↗

Immunization of mice with heat-solubilized hamster zonae: production of anti-zona antibody and inhibition of fertility.

Immunization of female mice with heat-solubilized hamster zonae pellucidae resulted in the production of antibody specific for the zona pellucida of several species, including that of primates. Complete, although temporary, infertility was produced in the mice. The zonae of ovulated eggs from the immune mice were found to contain antibody and complement. Many also appeared abnormal, but no apparent effect was observed on either vitelli or any of the other tissues that were examined. These results suggest the feasibility of developing a zona vaccine for contraception.

Animals↗

Receptor activity of the hamster and mouse solubilized zona pellucida before and after the zona reaction.

Capacitated golden hamster spermatozoa bound to the inner wall as to the outer surface of the zona pellucida, suggesting that the receptor-for-spermatozoa may occur throughout this egg envelope. When solutions of hamster zonae pellucidae were prepared by heating zonae in an aqueous buffer, receptor activity was retained and was stable to boiling. The addition of such solutions to capacitated spermatozoa (5 zonae/mul) prevented them from binding to eggs and fertilizing them. Solubilized mouse zonae were also partly effective, as would be predicted from the limited crossbinding between mouse and hamster gametes. Receptor activity was lost following the zona reaction.

Animals↗

(14C)Ethylenethiourea: distribution, excretion, and metabolism in pregnant rats.

Following administration of single oral doses of [14C]ethylenethiourea (ETU) to pregnant rats maternal blood maintained peak radioactivity for 2 h, and the radioactivity was dispersed uniformly between the red blood cells and plasma. The level of radioactivity was distributed equally among several maternal tissues but was present in lower amounts in embryos. Twenty-four hours after treatment all tissues examined, except blood, were relatively clear of radioactivity and 72.8% of the total radioactivity given had been excreted in the urine. Elution patterns of metabolites from Sephadex separation suggested that ethylenethiourea was degraded very little. The teratological mechanism is discussed.

Animals↗

Induction of the cortical reaction in hamster eggs by membrane-active agents.

Fusion of capacitated spermatozoa with the vitelline membrane, but not actual penetration, appears to initiate the cortical reaction in hamster eggs. The reaction can be artificially induced by the application of positively charged particles to the vitelline surface, a situation which may normally be prevented by the zona pellucida. Exposure of hamster eggs to neuraminidase, to lectins (concanavalin A and phytohaemagglutinin-P), to a monovalent ionophore (boromycin) and to 1,3-bis(4-chlorocinnamylideneamino)guanidine elicits a cortical granule discharge resulting in a block to fertilization. These agents all appear to act by inducing depolarization of the vitelline membrane.

Animals↗

Gas-liquid chromatographic headspace method for vinyl chloride in vinegars and alcoholic beverages.

Vinyl chloride (VC) is determined in vinegars and alcoholic beverages by gas-liquid chromatographic headspace analysis. The lower limit of detection is 10 ppb and confirmation by gas chromatography-mass spectrometry, single ion monitoring at m/e 62, is possible at this level. Comparison of the headspace and the direct injection methods for the determination of VC in vinegars and alcoholic beverages showed that the results obtained by the 2 methods were not significantly different (P greater than 0.05).

Acetates↗

Confirmation of vinyl chloride in foods by conversion to 1-chloro-1,2-dibromoethane.

A semiquantitative method has been developed for the confirmation of vinyl chloride (VC) in foodstuffs. The VC was brominated to give 1-chloro-1,2-dibromoethane which was analyzed by gas-liquid chromatography, using an electron capture detector. The yield of 1-chloro-1,2-dibromoethane varied between 40 and 55%, which permitted a lower limit of confirmation of VC of 15 ppb for vinegar and sherry and 50 ppb for peanut oil.

Chromatography, Gas↗

Gas-liquid chromatographic determination of vinyl chloride in alcoholic beverages, vegetable oils, and vinegars.

Vinyl chloride in foods is determined by gas-liquid chromatography either by direct injection of vinegars and alcoholic beverages or by headspace analysis of vegetable oils. The lower limit of detection is 10-15 ng/ml for direct injection and 1-10 ppb for headspace analysis. Confirmation by gas chromatography-mass spectrometry, single ion monitoring at m/c 62, is possible at 50 ppb for either method. The levels of vinyl chloride bottles were 0.0-1.6 mu-g/ml for alcoholic beverages, 0.0-8.4 mu-g/ml for vinegars, and 0.3-3.3 ppm for peanut oil.

Acetates↗