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Biomedical subjects

D T Burton

Publications and source records attributed to D T Burton.

At least 19 recordsLinked to original sources
PubMed2001

Effectiveness of the sulfur(IV) compound, sodium bisulfite, in reducing chlorine, chlorine dioxide, and chlorite toxicity to Daphnia magna in well water and pond water.

Flow-through toxicity tests were conducted with Daphnia magna to determine the residual toxicity of chlorine, chlorine dioxide, and chlorite after treatment with the sulfur(IV) compound sodium bisulfite. Daphnids were exposed separately to 0.5-mg/L concentrations of each of the three compounds without the addition of sodium bisulfite, with a low stoichiometric dose of sodium bisulfite, and with a high stoichiometric dose of sodium bisulfite. Tests were performed in well water with a low total organic carbon (TOC) content and pond water with a high TOC content. Analysis of results indicated that sodium bisulfite did not eliminate the toxicity of chlorine dioxide or chlorite to D. magna. Total residual oxidant (TRO) concentrations were reduced and survival times were extended, but acute toxicity persisted even with a S(IV) concentration 10.0 times the stoichiometric ratio of oxidant. Mortality occurred in chlorine dioxide treatments in which no TRO was detected, indicating that standard analytical (amperometric) techniques may be inadequate to detect toxicity. Sodium bisulfite did succeed in eliminating chlorine toxicity except in pond water receiving a low (3.0x) sodium bisulfite dose. Oxidant reactions with organic substrates may have produced chlorinated residuals that were resistant to S(IV) dechlorination.

Animals↗
PubMed1998

Phase III interlaboratory study of FETAX, Part 2: interlaboratory validation of an exogenous metabolic activation system for frog embryo teratogenesis assay--Xenopus (FETAX).

Interlaboratory validation of an exogenous metabolic activation system (MAS) developed for the alternative, short-term developmental toxicity bioassay, Frog Embryo Teratogenesis Assay-Xenopus (FETAX) was performed with cyclophosphamide and caffeine. Seven study groups within six separate laboratories participated in the study in which three definitive concentration-response experiments were performed with and without the MAS in a side-by-side format for each chemical. Since both chemicals had been previously tested in FETAX, the test concentrations were provided to each laboratory prior to testing. Interlaboratory coefficient of variation (CV) values for unactivated cyclophosphamide (no MAS) were 15%, 15%, 29%, and 25% for the 96-hr LC50, 96-hr EC50 (malformation), Minimum Concentration to Inhibit Growth (MCIG), and Teratogenic Index (TI) values, respectively. Addition of the MAS increased the CV values of each endpoint at least 3.9-fold. Interlaboratory CV values for unactivated caffeine were 31%, 18%, 31%, and 46% for the 96-hr LC50, 96-hr EC50 (malformation), MCIG, and TI values, respectively. Addition of the MAS decreased the CV values of each respective endpoint by at least 1.6-fold. Results indicated that bioactivated toxicants may be prone to greater variability in response amongst laboratories than compounds, which are detoxified. Even though more variability was noted with activated cyclophosphamide, results were within interlaboratory variation expected for other aquatic-based bioassays. Thus, results from these studies warrant the continued use and further refinement of FETAX for alternative developmental toxicity assessment.

Abnormalities, Drug-Induced↗
PubMed1984

Behavioral modification of estuarine fish exposed to sulfur dioxide.

This study was designed to determine the avoidance responses of juvenile striped bass (Morone saxatilis) and Atlantic menhaden (Brevoortia tyrannus) exposed to sulfur dioxide (sulfite) at acclimation temperatures of 15, 20, 25, and 30 degrees C. Predictive models were developed and compared for each species at each acclimation temperature. Striped bass avoided 2.2, 2.3, 3.0, and 3.5 mg sulfite/l at 15, 20, 25, and 30 degrees C, respectively. Atlantic menhaden avoided 3.2, 3.6, 2.9, and 3.0 mg sulfite/l at acclimation temperatures of 15, 20, 25, and 30 degrees C, respectively. Acclimation temperature was an important factor influencing the avoidance response of each species exposed to sulfur dioxide. Striped bass avoided lower concentrations of sulfite than Atlantic menhaden at 15 and 20 degrees C. Both species avoided approximately the same concentration of sulfite at 25 degrees C. Atlantic menhaden avoided lower concentrations of sulfur dioxide than striped bass at 30 degrees C.

Acclimatization↗
PubMed1984

Bioconcentration and metabolism of picric acid (2,4,6-trinitrophenol) and picramic acid (2-amino-4,6-dinitrophenol) in rainbow trout Salmo gairdneri.

The bioconcentration and metabolism of picric acid and picramic acid were determined for rainbow trout. The bioconcentration factor (BCF) in the epaxial muscle at 42 d for both of these compounds was less than 1; the skin had a BCF value of 1 and 9, respectively. The half-life (t1/2) elimination for the high and low dose of picric acid was 12.0 and 12.5 d, respectively; and for picramic acid was 9.0 and 9.5 d, respectively. In separate experiments, approximately 34% of the injected [14C]picric acid was metabolized to picramic acid, glucuronide conjugates, and an unidentified group of compounds, and 42% of the [14C]picric acid was metabolized to picric acid, glucuronide conjugates, and an unidentified group of compounds. The low bioconcentration in the trout muscle may be due to the trout's ability to excrete the parent compound and metabolites. The higher radioactivity observed on the skin may be due to the water route of exposure and the binding of the parent compounds to protein.

2,4-Dinitrophenol↗
PubMed1982

A comparison of the avoidance responses of individual and schooling juvenile Atlantic menhaden, Brevoortia tyrannus subjected to simultaneous chlorine and delta T conditions.

The objectives of this study were to determine and compare the avoidance responses of individual and groups of Atlantic menhaden, Brevoortia tyrannus, exposed to simultaneous elevated temperature (0, 2, 4, and 6 degrees C) and total residual chlorine (TRC) (0.00, 0.05, 0.10, and 0.15 mg/l) conditions. An unbalanced three-factor factorial design was used to develop predictive avoidance models with this species at a test temperature of 25 degrees C. Fish tested in groups elicited the following avoidance responses: (1) a high avoidance response occurred at 0.10 and 0.15 mg/l TRC regardless of delta T; (2) avoidance increased with increasing delta T values at 0.00 mg/l TRC; and (3) TRC was the most important factor influencing avoidance. Atlantic menhaden tested individually demonstrated the following responses: (1) avoidance increased with increasing delta T at 0.15 mg/l TRC; (2) minimal increase in avoidance at 0.00, 0.05, and 0.10 mg/l TRC as delta T increased; (3) greatest avoidance occurred at 0.10 mg/l TRC and 6 degrees C delta T; and (4) TRC was the most important factor influencing avoidance. A significant difference was found between avoidance models of the group and individual tests: greater avoidance generally occurred at most test conditions during the group tests. The data collected in this study showed the importance of testing methods when evaluating the avoidance responses of a strongly schooling species exposed to simultaneous chlorine and delta T conditions simulating a power-plant effluent.

Animals↗
PubMed1981

Seasonal temperature and power plant chlorination effects on estuarine invertebrates.

The influence of seasonal temperatures (15-22 degrees C) on chlorine (0.00, 0.15 and 0.30 mg/L total residual chlorine), delta T (2, 6 and 10 degrees C), and exposure duration (0.08, 2.0 and 4.0 hr) interaction studies simulating power plant entrainment and effluent discharge conditions was evaluated for opossum shrimp. Neomysis americana and amphipods, Gammarus Seasonal Temperature was an important factor effecting the toxicity of the three treatment variables with both of these organisms. Mortality responses were greater at the higher acclimation temperatures.

Animals↗