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D Staneková

Publications and source records attributed to D Staneková.

10 recordsLinked to original sources
PubMed2001

Pilot study of risk behaviour, voluntary HIV counselling and HIV antibody testing from saliva among inmates of prisons in Slovakia.

OBJECTIVE: To implement a pilot study of risk behaviour and HIV infection using HIV antibody testing from saliva to improve the situation as regards HIV/AIDS infection in prison institutions in the Slovak Republic. MATERIAL AND METHODS: The study comprised adult and juvenile males of grade one correction categories and prisoners from the prison for juveniles in Martin, as well as females prisoners in Nitra. Preventive activities were implemented in May 1998 in the form of discussions concerning topics related to HIV/AIDS infection. Saliva was collected for the presence of HIV antibodies and a questionnaire regarding sexual practice was completed. RESULTS: 32 persons [8 adult males (25%), 6 juvenile males (18.7%) and 18 females (56%)] were voluntarily tested for the presence of HIV antibodies in saliva. Nobody was HIV-positive. 75 persons (20 adult males, 30 juvenile males and 25 females) were involved in the study of risk behaviour. 40.8% participants had primary education, 28.2% secondary education, 2.8% were students of universities and 28.2% were apprenticies. 60% inmates (mostly females) were religious. Juvenile males reported the highest number of partners while females the smallest (p < 0.001). The more partners were reported by respondents, the lower was condom usage (p < 0.07). 47.6% females relied on credibility of partners, while 75% adult males and 50% adolescent males did not use protection. 0% females, 5% adult males and 10.3% juvenile males reported to have homosexual contacts outside prison while 19%, 5.6% and 8.3% in the prison, respectively. Paid sexual services were offered by 9.1% females, 15.8% adult males and 25% juvenile males. Outside prison adult and juvenile males used non-sterile used syringes as well as tattooing more often than females (p < 0.07 and p < 0.04, respectively). CONCLUSION: The present study provides information on the results of HIV-antibody testing in saliva. The results are based on a study of risk behaviour and difficulties linked with HIV/AIDS prevention among prisoners.

Adult↗
PubMed2000

HIV infection and sexual behaviour among homosexual and bisexual men in Bratislava.

OBJECTIVE: To determine sexual behaviour and HIV prevalence in a sample of homosexual and bisexual men in Bratislava. PATIENTS AND METHODS: Participants were recruited at gay discotheque in Bratislava in February and June 1996. Saliva samples were collected for testing the presence of anti-HIV antibodies and a questionnaire regarding sexual practice was completed. RESULTS: In the study 170 men (1st discotheque) and 124 men (2nd discotheque) were enrolled. The prevalence of antibodies against HIV was 5.4% and 1.6%, respectively. A group of 119 attenders (mean age: 25 years) was voluntarily questioned about their sexual lifestyle and related behaviour. Sexual orientation of participants was following: 66.4% homosexuals, 20.2% bisexuals, 13.4% others. Most responders were single and did not ever been married (86.55% vs. 12.6%, respectively). The figures of sexual intercourse were: steady partners: 45.38%, non-steady partners: 49.58%. More individuals were using condoms (70.58% vs. 15.97%, respectively), while only 28.57% were using them by each chance sexual intercourse. 6.7% responders suffered from an other STD and 3.36% were intravenous drug users. CONCLUSION: The HIV prevalence (5.4% and 1.6%) was relatively low. Considering the high prevalence of unsafe sexual practice further spread of HIV infection among homosexual men in Slovakia is to be expected.

Adult↗
PubMed1998

Antibody responses to the herpes simplex virus type 2 glycoprotein G in sera of human immunodeficiency virus-infected patients in Slovakia.

Thirty sera of human immunodeficiency virus-positive (HIV+) and 37 sera of HIV-negative (HIV-) individuals in Slovakia were tested for the presence of antibodies to herpes simplex virus type 2 (HSV-2) glycoprotein G (gG). A notable difference between the prevalence of HSV-2-specific antibodies in HIV+ and that in HIV- individuals was found (37% vs. 11%) confirming and extending previous reports that HSV-2 infection is an important risk factor for HIV transmission. Efforts toward the detection of HSV-2 infection and its therapy by anti-HSV drugs should be considered an important factor in decreasing the risk of contracting and spreading of HIV in Slovakia.

Animals↗
PubMed1993

Genetic polymorphism of factor B (Bf) and C3 component of complement in type 1 (insulin-dependent) diabetes mellitus: BFQO allele observed in a diabetic child.

C3 and Bf polymorphisms were studied in 215 and 192 children with type 1 diabetes mellitus (IDDM), respectively. No significant differences in C3 phenotypes and allele frequencies were found between IDDM patients and a healthy population. The rare allele BfF1 was found in 9.37% of diabetic patients but in only 0.35% of the general Slovak population (0.0468 vs. 0.0017). An increased frequency rate of BfSO.7 was also observed in 8.85% of IDDM patients compared with 3.57% of healthy controls (0.0442 vs. 0.0178). The relative risk was 28.83 for BfF1 and 2.55 for BfSO.7. One diabetic child was found to be heterozygous for a silent allele BfQO. This rare Bf allele was transmitted to the boy from his healthy mother.

Adolescent↗
PubMed1991

Genetic polymorphism of the C4 component of human complement in the Slovak population.

The distribution of C4 phenotypes and gene frequencies were studied in 104 genetically unrelated persons of Slovakia using high-voltage agarose gel electrophoresis with subsequent immunofixation. Five C4A alleles and three C4B alleles were detected. The gene frequencies were as follows: A2 = 0.0576, A3 = 0.7644, A4 = 0.0336, A6 = 0.0625, AQ0 = 0.817, B1 = 0.7836, B2 = 0.1009, BQ0 = 0.1153. The C4AQ0 and C4BQ0 alleles established by densitometry appeared in the Slovak population in 16.34% and 23.07%, respectively.

Adolescent↗
PubMed1990

Genetic polymorphism of factor B of the complement system (Bf) in the Slovak population.

The distribution of factor B (Bf) phenotypes and gene frequencies were investigated in 280 genetically unrelated persons of the Slovak population. Thin-layer agarose gel high-voltage electrophoresis and subsequent immunofixation were used. A low frequency of the "rare" allele BfFl was observed (BfFl = 0.0017). The frequencies of common Bf alleles BfS and BfF (BfS = 0.816, BfF = 0.1625) and a "rare" allele BfSO.7 (BfSO.7 = 0.0178) were inside the corresponding ranges of BfS, BfF and BfSO.7 found in European Caucasoids. No other variants were observed.

Alleles↗
PubMed1985

Pathogenesis of acute and persistent murine herpesvirus infection in mice.

Outbred laboratory mice were inoculated at the age of 5, 10 and 21 days by oral and/or intranasal routes with 2 different (a lethal and a nonlethal) doses of the murine herpesvirus isolate 68 (MHV-68). Severe exudative pneumonia with haematogenous dissemination of the virus to liver, heart muscle, and kidneys developed in the 5-day-old as well as in a part of the 10-day-old mice. Virus antigen was found by immunofluorescence (IF) in the alveolar lining of lungs, in heart muscle fibres, in spleen and thymic lymphocytes, in the tubular epithelium cells of kidneys, in the neurons of Gasserian ganglia and in the intima of large pulmonary vessels. Electron microscopy confirmed the transfer of virus particles through the capillary endothelium of the damaged alveolar septa. The surviving progeny and the mothers of animals, which had not succumbed to the lethal virus dose, were kept for 141-169 days when lungs and Gasserian ganglia were examined for virus presence. MHV-68 was recovered both by direct examination of the tissue homogenates as well as by the explantation technique. The results are suggestive for a dynamic persistence of MHV-68 rather than for static latency.

Animals↗
PubMed1984

Experimental pathogenesis of murine herpesvirus in newborn mice.

Newborn white mice were susceptible to peroral (p.o.) infection with murine alphaherpesvirus isolated from free-living Clethrionomys glareolus. Death occurred within 6-8 days in animals infected with the higher virus dose of 4.8 log10 TCID50. Clinical symptoms also occurred in some animals infected with lower doses, while others developed inapparent infection as judged by presence of humoral antibodies at 60 days post-infection (p.i.). The virus was detected in the lungs, blood, liver, spleen, kidneys, heart muscle, brain and urinary bladder of sick animals. Necrotising pneumonia accompanied the replication of the virus in the epithelial cells of alveolar ducts and alveolar lining as confirmed by immunofluorescence and histological examination. Latent infection of Gasserian ganglia in the survivors was not necessarily related to the administered dose of infectious virus. Two of mother females, which had eaten their diseased offspring, became inapparently infected as proved by reisolation of the virus from trigeminal ganglion explants and by detection of specific antibodies at 60 days p.i.

Animals↗
PubMedDate not supplied

Prevalence of antibodies to herpes simplex virus 2 among homosexual men either positive or negative for human immunodeficiency viruses in Slovakia.

We determined the prevalence of antibodies to herpes simplex virus 2 (HSV-2, HSV-2 antibodies) in sera of homosexual men either positive for human immunodeficiency virus 1 (HIV-1, HIV+, a group of 27 sera) or negative for HIV-1 and HIV-2 (HIV-, a group of 52 sera) in Slovakia. Antibodies to HSV-2 glycoprotein G-2 (gG-2, gG-2 antibodies) were determined by a double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) and immunoblot analysis. We found that 40% of HIV+ and 23% of HIV- homosexual men were positive for the gG-2 antibodies, what is 3.6 and 2.1 times higher incidence, respectively, than that in the control heterosexual population (Bystrická et al., Acta Virol. 42, 319-324, 1998). Identification of individuals infected with genital herpes among HIV+ and HIV- homosexual men should be succeeded by antiviral therapy in order to prevent transmission of HSV-2 and HIV as well in this community.

Antibodies, Viral↗