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Biomedical subjects

D Singh

Publications and source records attributed to D Singh.

At least 325 records · Page 18Linked to original sources
PubMed1980

Psoriatic arthritis: a clinico-radiological study.

A less documented entity of psoriatic arthritis was studied in 238 cases of psoriasis and it was detected in 5.1 % of cases. The prevalence of arthritis was found in the population over 40 years of age and was 2.7% of the males and 8.2% of the females. Psoriatic arthritis was much more common in females (male to female ratio = 1:2) in contrast to male predominance in psoriasis (male to female ratio = 1.6:1. Skin lesions usually precede the arthritis (50%); in others the onset is synchronous (41.7%) and in few of them the arthritis may come first (8.3%). Athritis is commonly encountered with moderate to severe involvement of skin (75%). In deforming type of arthritis the psoriasis was extensive and exfoliative (17%). The nails are more frequently involved at the onset of the arthritis. Rose-Waaler Test was negative in all of them. Anaemia (41.6%) and raised ESR (25%) was a feature in the active phase of the disease. Hyperurecaemia was noted in 41.6% of cases, indicating towards the extensive involvement of skin. The importance of radiological evaluation of spine, sterno-clavicular and sacro-iliac joints in all patients is stressed. Certain unusual features noted in the presented series of cases are; calcaneal spur (17%), spondylolisthesis (8.5%) and presence of LE cells (8.3%).

Adolescent↗
PubMed1980

Distribution and localization of adenosine 3':5'-monophosphate-dependent protein kinase in mammalian artery.

Among the three major vascular layers (the intima-inner, the media-middle smooth muscle, and the adventitia-outer connective tissue) over 90% of the total protein kinase activity was observed in the middle layer. Of various subcellular fractions of the vascular smooth muscle, the 105,000 g supernatant (cytosol fraction) showed the highest specific activity and represented more than two thirds of the total kinase present in this layer. DEAE-cellulose chromatography of the soluble enyzme revealed the existence of two major forms of cyclic AMP-dependent protein kinase, type I and type II, of which 60% of the total enzymatic activity was found in type II. A divalent cation was found to be essential for their phosphotransferase activity. Only Mg2+ and Co2+, but not Zn2+, Mn2+ or Ca2+ could satisfy the cation requirement. The phosphorylated substrate had the characteristics of a protein with a phosphoester bond.

Animals↗
PubMed1980

Methyl 5'-(6-aminopurin-9-yl)-5'-deoxy-beta-D-ribofuranoside 2',3'-cyclic monophosphate: a novel, biologically active, structural analogue of cyclic AMP.

A novel structural analogue of cyclic AMP has been synthesized. This compound has been found to activate protein kinase from skeletal muscle (Ka 5.0 microM). It is virtually resistant to degradation by beef heart cAMP phosphodiesterase. It is an inhibitor of this enzyme with an [I]50 of 47.0 microM. The proliferation of cancer cells (HT-29) is inhibited by this compound. It represents the first example of a 2',3'-cyclic nucleotide with marked biological activity.

3',5'-Cyclic-AMP Phosphodiesterases↗