Block co-polymeric magnetic resonance contrast agents.
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Animals↗
Biomedical subjects
D Shen
Publications and source records attributed to D Shen.
Comparison of PCR with culture for detection of Ureaplasma urealyticum in clinical samples from patients with urogenital infections.
PCR was compared with culture for the detection of Ureaplasma urealyticum in 50 specimens, including sperm, urine, and prostate secretions, from hospital patients with urogenital infections. Five positive and a further four doubtful diagnoses were made by culture, whereas PCR detected U. urealyticum in 12 samples. PCR also was faster than culturing. The increased sensitivity and shorter time requirement of PCR support its further development for the diagnosis of U. urealyticum infection.
Adult↗
[The biological properties of two CD71 McAbs].
Two monoclonal antibodies (McAbs), HI160 and HI166, recognize different epitopes of the CD71 antigen. Their biological properties have been studied, and the results demonstrated that both had down-regulation effects. HI160 McAb not only inhibited the activation and proliferation of T and B lymphocytes but also the growth of CFU-GM. HI166 McAb only inhibited the activation and proliferation of T lymphocytes. The differences in the biological properties of the two CD71 McAbs might be due to the different epitopes recognized by them. The application value of these McAbs is discussed.
Antibodies, Monoclonal↗
[Effect of xiaoyu pian on new platelet aggregation defect].
The Xiaoyu pian (XYP, mainly consisted of prunus persica, Carthamus tinctorius, Glycyrrhiza uralensis, etc) was used to treat patients with new platelet aggregation defect. Patients were divided into 2 groups, the TCM group (72 cases) treated with XYP and the control group (65 cases) treated with vitamin C and P and/or adrenosem for at least 3 months. The results showed that marked effective rate was 87.5% in TCM group and 35.4% in control group (chi 2 = 39.7, P < 0.01); the recovery rate of platelet was 91.8% in the former and 10.3% in the latter (chi 2 = 71.4, P < 0.01); the recurrence rate of follow-up (6 months after treatment) was 30.8% in the former and 82.1% in the latter (chi 2 = 19.2, P < 0.01). The difference between two groups was very significant. The results suggested that XYP could regulate the hemostatic action and the platelet aggregation function. It is worthwhile to use XYP as an hemostatic clinically.
Adolescent↗
[Effects of electroacupuncture on central and peripheral monoamine neurotransmitter in the course of protecting rat stress peptic ulcer].
The experimental Wistar rats were divided into two groups, the acupuncture group and the control group. Stress-induced gastric ulcer models were established by immersion of restrained rats in water. The results are as follows: 1. Stress-induced peptic ulceration was able to be protected with electroacupuncture. 2. Synthesis and decomposition of central and peripheral 5-HT in the electroacupunctured tats was inhibited. 3. The NE levels in the three brain regions (cortex, hypothalamus, brainstem) and blood were seen in the electroacupuncture group more than that in the control group, but the NE levels was smaller in the gastric tissue. 4. The higher levels of DA in the gastric tissue and blood were seen in the electroacupuncture group than in the control group. The results indicated that protecting rat stress ulceration with electroacupuncture was connected with monoamine neurotransmitter of centre and periphery.
Animals↗
Effects of hypoxia on platelet activation in pilots.
In order to evaluate the effect of hypoxia on platelet activation, 53 pilots were chosen for this study. Thirty were selected as the hypoxic group, and the other 23 as a control group. The plasma concentration of thromboxane B2(TXB2), 6-keto-prostaglandin f1 alpha (6-keto-PGF1 alpha), beta-thrombo-globulin (BTG) and platelet factor 4 (PF4) were measured during altitude chamber tests. After staying at 5000 m for 15 min, the plasma level of TXB2, BTG and PF4 increased significantly over pre-test levels (p < 0.01), but 6-keto-PGF1 alpha had no distinct change (p > 0.05). These changes indicate that moderate acute hypoxia causes increases in platelet activation, but has less effects on the endothelial cells.
6-Ketoprostaglandin F1 alpha↗
Percutaneous transluminal excimer laser coronary angioplasty. Clinical report of six cases.
Six patients with 95% to 100% occluded atherosclerotic lesions underwent percutaneous transluminal excimer laser coronary angioplasty (PTELCA). Among them, 5 were male and 1 was female; their age ranged from 28 to 66 years. Four patients had LAD stenosis and 2 LCX lesions. Acute angiographic and clinical success was achieved in all patients but one, with a success rate of 83.3%. It was demonstrated that PTELCA is a safe and effective therapy for selected patients with coronary artery disease.
Adult↗
[A high affinity CD3 monoclonal antibody HIT3a. I. Production and identification].
A monoclonal antibody (McAb, named HIT3a) against CD3 antigen of human T lymphocytes was produced by the mouse-mouse hybridization technique and identified by immunofluorescent test, immunohistochemistry assay, comparison with standardized McAbs, and some studies of biological properties. HIT3a McAb was found to have high affinity, good stability, complement fixation activity, specific cytotoxicity against T cells, and effective mitogenic activity on normal PBMCs. So it might be a good reagent for clinical and laboratory research.
Animals↗
Identification of the polypeptides of the major light-harvesting complex of photosystem II (LHCII) with their genes in tomato.
Using an improved SDS-PAGE system, the polypeptides of the major chlorophyll a/b light-harvesting complex of PSII (LHCII) from tomato leaves were resolved into five polypeptide bands. All the polypeptides were matched with the genes encoding them by comparing amino acid sequences of tryptic peptides with gene sequences. The two major LHCII bands (usually comigrating as a '27 kDa' polypeptide) were encoded by cab1 and cab3 (Type I LHCII) genes. A third strong band of about 25 kDa was encoded by cab4 (Type II) genes. Polypeptides from two minor bands of 23-24 kDa were not N-terminally blocked; their N-terminal sequences showed they were Type III LHCII proteins. One complete cDNA clone and several incomplete clones for Type III polypeptides were sequenced. Combined with the peptide sequences, the results indicate that there are at least four different Type III genes in tomato, encoding four almost identical polypeptides. Thus, all the LHCII CAB polypeptides have been identified, and each type of LHCII polypeptide is encoded by distinct gene or genes in tomato.
Amino Acid Sequence↗
Light particle emission near 0 degrees in the reaction 20Ne+181Ta at E/A=85 MeV.
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Journal Article↗
Nucleotide sequence and chromosomal location of Cab11 and Cab12, the genes for the fourth polypeptide of the photosystem I light-harvesting antenna (LHCI).
Tryptic peptide sequences from the 22 kDa polypeptide of tomato LHCI were used to construct a probe for gene cloning. The two genes cloned, cab11 and cab12, encode proteins of 251 and 250 residues that are 88% identical in overall amino acid sequence and 93% identical in the deduced mature protein. Each gene is present in a single copy per haploid genome; cab11 on chromosome 3 and cab12 on chromosome 6, and each has 2 introns located in similar positions to introns in other members of the Chl a/b-binding (CAB) protein gene family. Comparison of the amino acid sequences of LHCI, LHCII, CP29 and CP24 polypeptides confirms that all CABs share two regions of very high similarity which include the first and third transmembrane helices and the stroma-exposed sequences preceding them. However, near the N-terminus and between the conserved regions, the LHCI polypeptides have sequence motifs which appear to be PSI-specific.
Amino Acid Sequence↗
Proteolytic processing in a non-lysosomal compartment is required for transcytosis of protein-polylysine conjugates in cultured Madin-Darby canine kidney cells.
The transcytosis of horseradish peroxidase, as well as its poly(L-lys) and poly(D-lys) thioether conjugates, was investigated in Strain I Madin-Darby canine kidney (MDCK) cell monolayers grown on 0.4 microns pore size polycarbonate membranes in Costar Transwells. The 3 types of HRP had almost identical rates of transport during the first 2 hr of incubation. However, a significant increase of basal-to-apical transport was detected beginning at 3 hr only in Transwells containing the poly(L-lys) conjugate. This increase was inhibited by colchicine (2 microM) and by the Bowman-Birk protease inhibitor (0.1 mg/ml), but not by NH4Cl (10 mM) or chloroquine (0.1 mM). The increase was abolished either by prior trypsinization of the conjugate or by incubation at 4 degrees C. Ultrafiltration studies indicated that the transcytosed poly(L-lys) conjugate was smaller in size than the original conjugate. These results indicate that the conjugate was processed during transcytosis in a non-lysosomal proteolytic compartment, where its poly(L-lys) moiety was selectively degraded, allowing active peroxidase to be released into the apical medium.
Animals↗
In vitro synthesis of nitroxide free radicals by hog liver microsomes.
The in vitro biooxidation of 4-hydroxy-2,2,6,6-tetramethylpiperidine (TEMP), 4-hydroxy-2,2,4,4-tetramethyl-1,3-oxazolidine (TEMO) and diphenylamine (DPA) by hog liver microsomes to their respective nitroxide free radicals, 4-hydroxy-2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), 2,2,4,4-tetramethyl-1,3-oxazolidine-1-oxyl (TEMOO), and diphenylnitroxide (DPNO) has been investigated. For extending the life span of the liver microsomes, a calcium alginate immobilization procedure was used. The biooxidation rates of the above amines to their respective nitroxide metabolites were measured by means of oxygen uptake at 37 degrees C and pH 7.4. N-octylamine was found to be an activator in the biooxidation of the amines. The formation of the nitroxide radicals was identified by E.S.R. spectroscopy.
Alginates↗
Applications of a new chemiluminometric thyrotropin assay to subnormal measurement.
A new immunochemiluminometric TSH assay (ICMA) was shown to offer improved analytical (+2 SD of zero) and functional (20% interassay coefficient of variation) sensitivity [0.003 vs 0.045 +/- 0.005 (+/- SE; range, 0.01-0.07); 0.018 vs. 0.23 +/- 0.02 (range, 0.10-0.35, mU/L); analytical vs. functional sensitivity limit for the ICMA vs. 10 other TSH immunometric assays, respectively]. The ICMA was used to study the physiological relationship between serum TSH and free T4 [as reflected by free T4 index (FT4I)] values at both steady state and 14 days after acute pharmacological T4 administration (3 mg oral T4 load plus 0.3 mg daily). At steady state, an inverse log/linear relationship was found between serum TSH and FT4I values (log TSH = 2.56 - 0.022 FT4I; r = 0.84; P less than 0.001). Ten to 14 days after acute T4 suppression in 5 euthyroid subjects, serum TSH/FT4I levels had plateaued after decreasing in parallel to the slope of the steady state relationship, suggesting that the degree of T4 suppression of TSH can be predicted from an individual's pituitary TSH/free T4 set-point and the magnitude of the serum T4 elevation achieved. Ambulatory and hospitalized patient sera, previously identified as having low (less than 0.1 mU/L) TSH levels by a less sensitive assay, were restudied by the TSH ICMA. Normal TSH values ranged from 0.39-4.6 mU/L, whereas the majority of hyperthyroid patients [52 of 54 (96% ambulatory) and 22 of 23 (96%, hospitalized)] had undetectable (less than 0.005 mU/L), basal TSH levels and absent TRH stimulated TSH responses. In contrast, most (32 of 37; 86%) of hospitalized nonhyperthyroid patients with low (less than 0.1 mU/L) TSH values due to nonthyroidal illness or glucocorticoid treatment had detectable (greater than 0.01 mU/L) basal and TRH stimulated TSH levels. The positive relationship between basal and TRH-stimulated TSH levels was shown to extend down to the detectability limit of the assay (0.005 mU/L), which further supported the authenticity of the subnormal TSH ICMA measurements. The new TSH ICMA is considered to represent the first of a third generation of clinical TSH assays, since it has a functional (interassay) sensitivity that is 2 orders of magnitude greater than that of typical first generation TSH RIAs and 1 order of magnitude greater than current second generation TSH immunometric methods. Such third generation TSH assays will facilitate both the optimization of T4 therapy as well as the diagnosis of hyperthyroidism in hospitalized patients with nonthyroidal illness.
Adult↗
[Changes in the levels of human platelet membrane glycoproteins in patients with a new-type defect of platelet aggregation].
Labeled avidin-biotin (LAB)-enzyme linked immunosorbent assay (ELISA) for quantitative measurement of the levels of human platelet membrane glycoproteins (GPs) Ib, IIb and IIIa was developed by using a group of murine monoclonal antibodies raised against human platelet membrane GPs. The contents of the GPs Ib, IIb and IIIa in patients with a new-type defect of platelet aggregation were detected and found to be in normal range. However, the amounts of GPs IIb and IIIa before washing in patients were significantly decreased in these patients when compared with those in controls (P less than 0.01), while after washing, normal levels could be obtained. The results reported have support the hypothesis that the pathogenesis of this defect in platelet aggregation seems not to be associated with the abnormality of the levels of GPs, but is a result of abnormal factor existing in the plasma. Further investigation on the properties of the factor is in progress.
Blood Platelet Disorders↗
[An observation of protective effect of acupuncture on the gastric mucosa of Wistar rats and the relative histochemical changes of the neurotransmitters].
In this paper, changes of neurotransmitters and the protective effect of acupuncture on gastric mucosa have been studied by the histochemical methods of cholinesterase and catecholamine on 30 pairs of Wistar rats. 1. Sequential medical trials on rats of water-immersion induced gastric ulceration show that there was a protective effect of electro-acupuncture on gastric mucosa and it was statistically significant. 2. Under the protective effect of acupuncture on gastric mucosa, finally, both the cholinergic and adrenergic nerves undergo significant inhibition.
Acupuncture Therapy↗
[Binding of acid mucopolysaccharides of Stichopus japanicus to washed human platelets].
The binding of acid mucopolysaccharides of Stichopus japonicus Selenka (Sjamp) to washed human platelets was studied using [3H]Sjamp. The binding was rapid, reversible and with a saturable tendency. The binding reached to equilibrium at about 10 min, and 50% of [3H]Sjamp bound to the platelets at equilibrium within the first minute. When excess unlabeled Sjamp was added to the [3H]Sjamp binding suspension, [3H]Sjamp dissociated from the platelets rapidly and rather completely. Scatchard analysis revealed a class of binding sites with Kd = 2.3 +/- 0.7 micrograms/ml and Bmax = 4.5 +/- 1.2 micrograms/10(9) platelets. Separate experiments showed that the monoclonal antibody (McAb) SZ-21, a McAb to platelet membrane glycoprotein (GP) IIIa, weakened the binding, while McAb SZ-2 and SZ-22 (to GP Ib and GP IIb respectively) were ineffective. We also observed that heparin inhibited [3H]Sjamp binding to the platelets. These results indicated that the binding sites on platelets might be the Sjamp receptors. We conclude that receptor-Sjamp binding is an important process in platelet aggregation induced by Sjamp.
Animals↗
Detection of coronavirus-like particles from mink with epizootic catarrhal gastroenteritis.
Coronavirus-like particles have been detected by electron microscopy in fecal samples from naturally occurring cases of epizootic catarrhal gastroenteritis (ECG) of mink. Preliminary transmission trials with bacteria-free filtrates from mink with ECG suggested that a coronavirus plays a role in the disease syndrome.
Animals↗