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Biomedical subjects

D Shah

Publications and source records attributed to D Shah.

150 records · Page 9Linked to original sources
PubMedDate not supplied

Widespread bone disease in acute myeloid leukaemia.

We describe a 11-year-old boy with acute myeloid leukaemia who presented with widespread bone disease. Spine X-rays revealed multiple crush fractures and there were multiple hot spots on the bone scan. The bone-mineral density was markedly reduced but there was no hypercalcaemia or hypercalcuria. Bone marrow aspirate revealed 98% blast cells and a balanced translocation between chromosomes 10 and 17 in seven of nine metaphases. Plasma interleukin-6 level before chemotherapy was high at 53 pg/ml. We postulate that the mechanism for bony destruction in this case was similar to that in the adult disease myeloma.

Bone Density↗
PubMedDate not supplied

Alkylation of tryptophan during deprotection of Tmob-protected carboxamide side chains.

Alkylation of tryptophan occurred during the synthesis of a 19-amino acid peptide of the following sequence: Tyr Ala Ala Gln Asn Arg Arg Gly Leu Asp Leu Leu Phe Trp Glu Gln Gly Gly Leu. Alkylation was found to occur during cleavage of the peptide from the solid support under acidic conditions in the presence of Tmob, which is used for protecting glutamine and asparagine. Presence of alkylated tryptophan was confirmed by mass spectrometry, HPLC and amino acid sequence analyses.

Alkylation↗
PubMedDate not supplied

Significance of ST alternans during percutaneous transluminal coronary angioplasty.

Repolarisation ST alternans was observed during percutaneous transluminal coronary angioplasty (PTCA) in nine patients. Six had ST alternans on surface electrocardiogram (ECG) while three patients developed ST alternans only on intracoronary ECG recordings. The pattern of occurrence of ST alternans in successive inflations varied. Ventricular arrhythmias occurred in 6 of 28 (21.42%) of inflations associated with ST alternans while in a control group of 50 patients undergoing PTCA who did not have ST alternans, ventricular arrhythmias were noted in only 6 of 342 inflations (1.75%; p < 0.001). It is suggested that ST alternans is a regional ischemic phenomenon.

Adult↗
PubMedDate not supplied

The effects of various excipients on the unfolding of basic fibroblast growth factor.

The purpose of this study was to determine the effects of various pharmaceutical additives on temperature and chaotrope-induced (guanidine HCl = GnHCl) denaturation of basic fibroblast growth factor (bFGF). The effect of various pharmaceutical additives on the stability (tertiary structure) of bFGF was assessed using fluoresence spectroscopy. An increase in the hydrophobicity of anionic surfactants (alkyl sulfonates) incubated with bFGF were shown to retard thermal-induced denaturation of bFGF. Increasing the concentration of decane sulfonate in an aqueous bFGF solution decreased the rate and extent of GnHCl-induced denaturation and demonstrated no inherent capacity to significantly alter the conformation of bFGF by itself. Structural differences in surfactants (e.g., aromatic, heterocyclics and straight-chain alkyl compounds) resulted in differences in the rate and extent of thermal-induced denaturation of bFGF. An increase in the concentration of citrate buffer increased the rate and extent of thermal-induced denaturation of bFGF. Results suggest that various excipients may affect the rate and extent of bFGF unfolding induced by chemical or thermal stress. Ionic charge and the degree of lipophilicity of an additive may significantly affect the rate and extent of the unfolding process.

Fibroblast Growth Factor 2↗
PubMedDate not supplied

Gastric outlet obstruction secondary to post cholecystectomy biloma: case report and review of the literature.

BACKGROUND: Postcholecystectomy bilomas are relatively uncommon with a reported incidence of about 2.5%, and most often present with right upper quadrant pain and fever within seven days of the operation. There are a number of approaches to the treatment of this uncommon lesion. CASE REPORT: The authors report a case of a cholecystectomy performed in a 35-year-old female which resulted in a biloma, presenting 11 days postoperatively with gastric outlet obstruction. The literature is reviewed to suggest the optimal management and treatment plans for this complication. RESULTS: This patient was successfully managed with radiologic intervention for the treatment of a biloma and resulting gastric outlet obstruction after open cholecystectomy. CONCLUSION: Although uncommon, bilomas may present in an unusual manner such as with gastric outlet obstruction. As with early bilomas, they may be treated successfully with interventional radiologic techniques.

Adult↗