Biomedical subjects
D Seroka
Publications and source records attributed to D Seroka.
[Animal sources of rabies infection in Poland in 1985-1994. Epizootiological tendencies and dynamics of the focus before the introduction of mass wildlife oral antirabies vaccination].
The purpose of this paper is to collect (four tables), evaluate and map (two figures) the numbers and dissemination of rabies among animal species in Poland during the last ten years. This information is required for planning, organizing and implementing of antirabies wildlife oral vaccination and for evaluation of their effectiveness.
[Antigenic determination of human anti-rabies vaccine against viral street strains common in the wild animal population in Poland].
The aim of the study was to compare the antigen properties of a vaccine strain with street strains isolated from various animal hosts throughout the country. Investigation was carried out using monoclonal antibodies against NC protein. Also, two tests were carried out: the modified NIH test for potency and the neutralization test using the sera of people vaccinated against rabies (PM vaccine strain). The investigated street strains were used in both tests as the challenge viruses. A suspension of these strains diluted five times made it possible to avoid extreme values of animal survival (0% or 100%) what, consequently, made calculation of the LD50 value easier. A different rabies virus serotype (EBLI virus) in the population of insectivore bats Eptesicus serotinus and antigen variants within the first serotype, having common epitopes with strains of the vaccine virus SAD B19 and the polar rabies virus, were found to be present throughout the country. The concentrated and purified vaccine containing the PM virus did not protect mice against infection with strains of viruses isolated from bats (protection index 10 and lower). For the remaining strains, depending on the animal source of their isolation, the protection index ranged from 10 to 1000 and higher. The properties neutralizing a dose of 5 i.u./ml of serum from the subject inoculated with the vaccine containing the PM strain were similar for all the investigated strains; 0,5 i.u./ml did not neutralize the strain isolated from a racoon dog.
[Isolation and multiplication of rabies virus in cell lines under conditions of routine rabies diagnosis].
Isolation of rabies street virus from brains of animals with rabies was performed on cells of mouse neuroblastoma, infecting cells with suspension of tested brain tissue diluted 1:10, incubating at 37 degrees C in standard incubator (culture in Legroux bottles and Leighton tubes with rubber stoppers) and in 37 degrees C in an incubator with moist chamber and regulated flow of carbon dioxide (Mirco-Well plates, bottom-flat tubes, bottles produced by Nuc, 25-chamber plates produced by Flow). The main conditions for obtaining positive results was proper selection and composition of growing fluid for cells (MEM with Earle's salts produced by Sigma enriched with vitamins, L-glutamine, amino acids and calf fetal fluid) and limited number of passages of tissue after thawing cells from the bank. With passaging neuroblastoma cells are becoming resistant to infection. Growth of cells abd isolation of the virus were possible both in atmosphere containing 5% CO2 and normal air, but application of a standard incubator required higher usage of growth fluids. Positive results were also obtained with infected material stored for a long time. Storage of material at 37 degrees C was making isolation more difficult. Multiplication of stable viruses on BHK, neuroblastoma and Vero cells permitted for obtaining antigens for serological diagnosis of rabies in vitro.
[Rabies in 1992].
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[Brucellosis in 1992].
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[Evaluation of epidemiologic surveillance of human brucellosis-- occupational disease overcome in the midst of animals].
National eradication programme of bovine Brucellosis in Poland has reduced prevalence below 0.5%. The last cow-abortion incidence caused by Brucella abortus bovis was recognized in 1982. The registered cases of human brucellosis (610 during 1986-1992) represent chronic occupational infections in man acquired earlier, when bovine brucellosis was common in Poland. Actually, some of victims show no serological evidence of infection. Present small risk of infection permits to stop or to limit (only veterinarians) continuous (enough expensive) prospective serological examinations of occupational groups exposed to possible brucellosis in Poland. The interchange of information and surveillance data between Veterinary and Health Service is mostly essential for prevention of humans. When brucellosis is suspected or reported among animals--the medical service should be alerted to the possibility of the human infection.
[Factors constituting a threat to humans of rabies in Poland].
Today, there are two steps of human rabies impendence: a. exposures to rabid or rabies suspected animals b. human deaths due to rabies. Red foxes, dangerous rabies reservoir are responsible for the main tendency and spread of rabies in Poland (over 2000 cases in 1992). This has a direct bearing on the treatment of exposed persons and on rabies among unvaccinated domestic animals. The number of persons requesting post exposure rabies treatment is also increasing--6500 vaccinees in 1992. During last eight years no case of human death was registered in Poland. Mass parenteral canine vaccination campaign and effective control methods of rabies in dogs in Poland are the mostly successful and significant elements separating wild animal rabies from human society. Any inaccuracy on this field would be the risk of transmission of wild rabies to human surrounding.
[Qualifying individuals for vaccine against rabies and administration of the vaccine in Poland from 1980-1990].
In Poland are implemented five basic elements necessary for the efficient human rabies control. They are: epidemiological surveillance of rabies; safe and immunogenic vaccine and serum; effective medical care; network of veterinary diagnostic laboratories; rabies legislation. Presented analysis is based on the 35,226 records of antirabies vaccinees in Poland during the period 1980-1990. It evaluates some failures in human antirabies control. The efforts to achieve the improvement in the standard of the performance should focus on the following: the most effective methods of increasing the level of public knowledge about rabies; medical postgraduated training in rabies; rapid methods for virus isolation; virological evidence (virus isolation) of infection in suspected atypical rabies hosts like squirrels, rodents, bats etc; pre-exposure vaccination for persons at risk of exposure.
[Safety assessment of rabies vaccine strains used for oral wild animal immunization].
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