An improved Giemsa C-banding procedure for plant chromosomes.
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Biomedical subjects
Publications and source records attributed to D Schweizer.
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The karyotype of the European wild pig (Sus scrofa scrofa L.) was analysed by means of silver-staining and the chromomycin A3/distamycin A/DAPI fluorescent banding technique to locate active NORs and to differentiate types of C-bands. The ribosomal RNA genes are localized at the secondary constrictions of chromosomes 8 and 10. All biarmed chromosomes, with the exception of chromosome 15/17 and the Y, had a chromomycin bright centromeric region that was moderately fluorescent with distamycin A/DAPI (DA/DAPI). Conversely, all acrocentric chromosomes and the Robertsonian fusion product (15/17) exhibited DA/DAPI bright centromeric heterochromatin. The results are compared with the chromosomal staining behavior of the domestic pig (Sus scrofa domestica L.) and discussed with respect to presumptive mechanisms of karyotypic evolution.
Synaptonemal complexes (SCs) have been analyzed in spread Xenopus spermatocytes and oocytes. They showed all the usual features of animal SCs in addition to a high incidence of centromere mismatching. A centriole pair is visible throughout zygotene and pachytene. At zygotene the ends of SCs are markedly thickened and are clustered at the nuclear periphery.