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Biomedical subjects

D Schwarz

Publications and source records attributed to D Schwarz.

At least 37 records · Page 2Linked to original sources
PubMed1996

[Selective chemical modification of cytochrome P450scc (CYP11A1) with diiodofluorescein iodoacetamide in the study of the role and topology of interdomain hinge of the hemoprotein molecule].

Bovine adrenocortical cytochrome P450scc (CYP11A1) was selectively modified with diiodofluorescein iodoacetamide (DIFIA). Only Cys264 is labeled in the P450 polypeptide chain. The modification significantly affected the cholesterol-hydroxylating activity in the reconstituted system containing NADPH, adrenodoxin reductase, adrenodoxin, and soluble or membrane-bound P450scc. The inhibitory effect correlates with decreased affinity of cytochrome P450scc to intermediate electron carrier, adrenodoxin. Cytochrome P450scc is modified in liposomes and the modified membrane-bound protein is cleaved by trypsin forming two large fragments F1 and F2 corresponding to the N- and C-terminal regions of the molecule. The data indicate that the Cys264-containing region of the cytochrome P450scc molecule is exposed to the surface of protein globule, located outside of the membrane, and can participate in protein-protein interactions.

Adrenal Cortex↗
PubMed1995

Laparoscopic Swenson pull-through procedure for congenital megacolon.

Constipation that is unresponsive to conventional remedies is the primary symptom of congenital megacolon (ie, Hirschsprung's disease). The cause of congenital megacolon is lack of ganglion cells in the bowel. The laparoscopic Swenson pull-through procedure involves removing the aganglionic segment of the colon, bringing the normally decompressed bowel through the pelvic floor, and anastomosing the bowel to the anorectal verge. Advantages of the laparoscopic approach include shorter lengths of hospital stay and fewer complications resulting from disruption of skin integrity.

Anastomosis, Surgical↗
PubMed1994

Screening for hereditary hemochromatosis in prospective blood donors.

Prospective blood donors (n = 1,265, mean age 26 years) were screened for elevated serum ferritin and serum iron. Final diagnosis for hereditary hemochromatosis was made by liver iron concentration (noninvasive biomagnetometry), transferrin saturation, and 59Fe absorption in 3 male subjects. This preliminary result confirms for the first time the current frequency estimation of homozygous hemochromatosis (0.2-0.6%) in a group of young North-Germans.

Adult↗
PubMed1993

Rotation of cytochrome P450SCC (CYP11A1) in proteoliposomes studied by delayed fluorescence depolarization.

The rotational diffusion of cytochrome P450SCC (CYP45011A1) was investigated in proteoliposomes measuring the time-resolved delayed fluorescence anisotropy of diiodofluorescein iodoacetamide covalently and specifically attached to Cys264 of P450SCC. Rotation strongly depends on the lipid composition of the membrane, especially on the cardiolipin content. In proteoliposomes having a lipid composition similar to the inner membrane of bovine adrenal mitochondria P450SCC is rotating uniaxially with a relaxation time phi rot about 53 microseconds and almost no immobile P450 is present. Addition of high KCl concentration has no effect. The results and the absence of any intramembrane particles observed by freeze-fracturing indicate that P450SCC probably exists in the liposomal membrane as oligomer not penetrating the bilayer. It is suggested to bind tightly within the outer monolayer with large parts exposed to the aqueous solution surrounding the membrane.

Adrenal Glands↗
PubMed1993

Adolescent Trauma: Epidemiologic Approach.

Trauma caused by all types of injury is the leading reason for visits to the emergency department, hospital admission, and death among adolescents in the United States. Injury related to motor vehicles is the predominant killer, followed by homicide, suicide, drowning, and firearm accidents. In addition, the rate of injuries resulting from sports (both organized and unsupervised) and recreational activity increases throughout adolescence. The task for the physician in the emergency setting is not only to treat bodily damage but also to introduce the subject of prevention and to assess other injury risks for a given patient.

Journal Article↗
PubMed1993

[Complex focal seizures as a cause of long-term paranoid psychoses in advanced age].

Many organic diseases of the brain--including intoxications, inflammatory or neoplastic disorders--can cause acute psychosis. Even if the psychotic symptoms last for several years, the possibility of an organic cause should be borne in mind. We report the case of a patient with a paranoid disorder for two years before admission. The EEG showed generalized 1-2 Hz spike and wave complexes. These epileptic activities--and also the paranoid symptoms--ceased completely after administration of an antiepileptic medication.

Aged↗
PubMed1992

Expression of bovine adrenodoxin in E. coli and site-directed mutagenesis of /2 Fe-2S/ cluster ligands.

Expression systems for adrenodoxin into the periplasm and the cytoplasm of E. coli have been developed as a prerequisite for site-directed mutagenesis studies. In both systems the /2Fe-2S/ cluster of the protein was correctly assembled, the cytoplasmic one gives, however, a tenfold higher expression level. To determine which of the five cysteines at positions 46, 52, 55, 92, and 95 coordinate the /2Fe-2S/ center, they have been individually mutated into serines. From these mutants, only C95S forms a functionally active holoprotein. Thus, residues 46, 52, 55, and 92 are the cysteines that coordinate the /2Fe-2S/ cluster in adrenodoxin.

Adrenodoxin↗
PubMed1991

Novel method of purification of human leukocytic elastase using adsorption on a high-performance liquid chromatography gel permeation column.

Neutrophil elastases are serine proteinases released during acute and chronic inflammatory states. We have developed a novel isolation method for neutrophil elastase, involving conventional gel chromatography followed by adsorption of protein at low ionic strength on a high-performance liquid chromatography gel permeation column. The bound elastase is then eluted by application of higher ionic strength. This adsorption step at low ionic strength, a step to be avoided in most purification methods, was used to advantage here to allow isolation of homogeneous material. This purification procedure should be useful for quick, simple bulk preparation of the enzyme.

Chromatography, Gel↗
PubMed1991

Polyglyconate suture in pediatric surgery.

The authors have clinically evaluated a synthetic monofilament suture and have found it very satisfactory for all wound closures, esophageal and intestinal anastomoses, and bronchial closures.

Adolescent↗
PubMed1990

Membrane topology of microsomal cytochrome P-450: saturation transfer EPR and freeze-fracture electron microscopy studies.

The rotation of cytochrome P-450 LM2 (CYPIIB4) incorporated into large microsomal-like lipid vesicles was investigated by saturation transfer EPR using 15N- and 2H-substituted spin labels. In combination with rotational diffusion, the distribution and size of protein particles in the bilayer were studied by freeze-fracture electron microscopy. The data from both methods suggest an oligomeric and membrane-spanning aggregate for the topology of microsomal cytochrome P-450.

Cytochrome P-450 Enzyme System↗
PubMed1990

[Jewish dietary laws and health education].

We divide the statute of foods in the real food-statute and in the rules about the cooking. Particularly the food-statute, we find in the bible (Leviticus chapter 11 and Deuteronomium chapter 14), demonstrated education of health already, in the antiquity. Essentially the jewish food-laws instruct us in the severe training of temperance and discipline. But that system of education teach men also how they reduce the fat of the animals, which are permitted to eat. Likewise the jewish rules refer to the importance of temperance of alcoholic drinks. The empiric knowledge of reduction and selection of animal food effect a theoretic basis leading to the prophylactic and to the preventive of civilization disease and of cancer development.

Diet↗
PubMed1990

[Alimentary intake of fluorides of artificially fed infants and effect on enamel tissue].

The dietary daily intake of fluorides in the first six months of lifetime has to be taken into special consideration. The slightly toxicological permissible limits of the fluorides can cause mottled enamels. The safe amount of the dietary daily intake for babies ranged up to 0.5 mg. By use of the food-stuff "MANASAN" prepared with fluoridated drinking water the daily intake increased up to 1.01 mg F-. In 150 7-8-years old children which in the first 4 month of lifetime got "MANASAN" prepared with fluoridated drinking water we found in 40% of the children mottled enamels, the fluorosis index scores (defined by DEAN) 0.4 respectively. The caries prevalence was by 0.85 DMF surfacer index.

Bottle Feeding↗
PubMed1990

[Historical aspects of the therapy of supracondylar humerus fractures].

Historical review in treatment of supracondylar numerous fractures. The treatment of fracture of the supracondylar humerus is already published in the antiquity. The statistic of medical attendance demonstrates, that the most authors claim the arrangement of the fracture immediately by extension of the forearm in inflexion of 90 grade. The best retention is the immobility of the arm by inflexion of 90 grade. Moreover it is necessary to inspection daily and if required the new reposition. Today the historical aspects instruct, the most frequent treatment is the extension at the right-angled flexed forearm and the retention in the same position.

Europe↗
PubMed1989

Role of lipid in the electron transfer between NADPH-cytochrome P-450 reductase and cytochrome P-450 from mammalian liver cells.

1. The anaerobic NADPH-reduction of the isozymes cytochrome P-450 LM2 and LM4 was used as a functional tool to study the component interaction in reconstituted monooxygenase systems in dependence on different phospholipids. 2. The isozymes were shown to exhibit similar lipid interaction. The lipids generally favour a catalytically active 1:1 complex formation between reductase and cytochrome P-450 as the rate-determining unit in electron transfer. 3. The cytochrome P-450 reduction proceeds in a biphasic reaction. In dilauroyl phosphatidylcholine (DLPC)-reconstituted systems the amount of the fast reduction psi 1 is stoichiometrically limited by the reductase in deficit: psi 1 corresponds to the 1:1 complex formation capability of the reductase. 4. In vesicle-reconstituted systems an 'overstoichiometric' reductase cycling is observed which gives rise to a significantly increased amount of fast reduction psi 1. Reductase cycling is proposed to occur in protein clusters of cytochrome P-450 and reductase in deficit. 5. The dissociation constant KRP of the functionally active reductase-cytochrome P-450 complex has been determined by means of the amount of psi 1 (DPLC) and the rate constant kapp 1 (vesicles) of the fast reduction as a measure of the complex formation in dependence on the protein molar ratio. Taking into account the actual protein concentration in the vesicular lipid phase, KRP in vesicles has been calculated to be about 3 orders of magnitude increased in comparison to DLPC-reconstituted systems. 6. Vmax data reveal almost the same catalytic activity of both reconstitution modes, which justifies DLPC-reconstitution in model investigations. The vesicle-specific increased accumulation of reduced cytochrome P-450 in the steady state as originated by reductase cycling may offer the physiological advantage of an increased capacity of cytochrome P-450 for synergistic substrate conversion via cytochrome b5.

Animals↗
PubMed1989

[Historical observation of indications for amputation of the hand].

In passing the indication of amputation of hand region in review the attitude appears not much changed for the beginning of modern surgery in the 16. und 17. century. Since that time a roll-call is published to the medical ethics and the medical exactness even for this simple therapeutic method. Many years ago and in these days the surgery treatment trends towards possibilities of conservation of hand or its parts and towards the replantation of extremities. But the present state of patient always have to take into consideration. To day amputations of hand region because of juridical indication are rare.

Amputation, Surgical↗
PubMed1988

HLA-DQ molecules form alpha-beta heterodimers of mixed allotype.

Retroviral vectors with an internal cytomegalovirus major immediate-early gene enhancer/promoter regulating HLA class II gene expression were used to transfer HLA cDNA into human EBV-transformed B-lymphoblastoid cell lines. HLA-DQ2 beta and DQ3.2 beta cDNA were transferred into DQ3.2 and DQ2 homozygous lymphoblastoid cell lines, respectively. Serologic analysis of the infected cell lines with allospecific mAb demonstrated surface expression of these exogenous DQ molecules implying that DQ alpha-chains from DR3, DQ2-positive cells can pair with DQ3.2 beta-chains and, similarly, DQ alpha-chains from DR4, DQ3.2-positive cells can pair with DQ2 beta-chains. Immunoprecipitation of the introduced DQ3.2 beta molecule resulted in co-purification of the allotype-mismatched endogenous DQ2 alpha polypeptide. We also show that vectors with a cytomegalovirus major immediate-early gene enhancer/promoter result in higher levels of expression of the transduced gene compared to previously described HLA vectors with either the SV-40 early enhancer/promoter or the lymphotropic papovavirus-enhanced SV-40 promoter. Although deletion of HLA cDNA did sometimes occur in the process of generating virus-producing clones, the HLA cDNA is stably maintained in virus-producing clones, once it is generated. This retroviral expression system is a highly efficient way to study HLA gene function.

Cell Line↗
PubMed1988

[Menstruation and the woman in Judaism].

Since ancient time subtile cultic regulations have been fundamentals of the habits of jewish people. Women had been affected from these in a considerable degree, consequently pressed into a social exceptional position. The laws of cleanness about behaviour during menstruation have been joined on a large scale. Nevertheless, behind the religious demands are a actual quinding directives of hygiene and timeless ethical principles. The question for prophylactic hints enclosed there cannot be answered.

Female↗