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Biomedical subjects

D Schwab

Publications and source records attributed to D Schwab.

At least 73 records · Page 4Linked to original sources
PubMed1987

Treatment of metastatic malignant melanoma with recombinant interferon alfa-2b.

Twenty-six patients with histologically proven metastatic malignant melanoma were included in a phase II trial of interferon alfa-2b (Intron A; Schering-Plough). Patients were given 10 X 10(6) IU/m2 of interferon alfa-2b subcutaneously three times a week until major intolerance or progression of disease. General signs of intolerance were seen in all patients; hematological toxicity with leukopenia (below 1,800/mm3) and/or thrombocytopenia (below 600/mm3) was seen in six patients and therapy was interrupted in one patient. Mild liver toxicity was seen in most patients after two weeks of treatment. These manifestations disappeared 1-2 weeks after treatment was discontinued. Twenty-four patients were evaluable for response. There were two complete responses; one skin and one lymph node going into remission for 12 and 12.5 months respectively. A partial response was observed in five cases lasting 1, 1.8, 2, 3 and 5 months respectively. These results indicate a potential role for interferon alfa-2b in treating patients with metastatic malignant melanoma, however, further trials are required to determine the optimum dose and schedule of administration and use of interferon alfa-2b in combination with cytotoxic drugs.

Drug Evaluation↗
PubMed1986

Clinical phase II trial of recombinant DNA interferon (interferon alpha 2b) in patients with metastatic malignant melanoma.

Twenty-four patients with histologically proven metastatic malignant melanoma were included in a Phase II trial of human DNA recombinant interferon (rDNA IFN alpha 2). They were given 10 X 10(6) IU of IFN alpha 2 subcutaneously three times a week until progression of disease or major intolerance developed. Twenty-two patients were evaluable for toxicity and response. General manifestations of intolerance were seen in all the patients. Hematologic toxicity was seen in six patients and therapy had to be interrupted in one patient. Mild liver toxicity was seen in most patients after 2 weeks of treatment. These manifestations disappeared within 2 weeks after treatment was discontinued. A partial response was seen in four cases lasting 2, 4, 4, and 5 months, respectively. There were two complete responses (one skin, one lymph node metastasis) lasting 20 and 6 weeks, respectively. These results indicate a potential role for rDNA IFN alpha 2 in treating patients with metastatic malignant melanoma. However, further trials are required to determine the optimal dose and schedule of administration and modalities of combination.

Adult↗
PubMed1983

Isolation of ;Vacuoplasts' from Poterioochromonas malhamensis.

A method is reported for the isolation of ;vacuoplasts' from Poterioochromonas malhamensis. Vacuoplasts are separated mechanically by centrifugation on silica-sol gradients. They consist of the leucosin storage vacuole, a portion of the plasma membrane, and some cytoplasmic components. This method is suited to give a high yield of vacuoplasts.Vacuoles are the largest membrane-bound organelles in plant cells. Only a few methods exist for their large scale isolation and purification in an intact and physiologically active state. The high shear forces used to disrupt cell walls during tissue fractionation usually also disrupt the fragile vacuoles. Intact vacuoles have been isolated from root storage tissue of Beta vulgaris L. (14) by a slicing procedure or by lysing enzymically prepared protoplasts from yeast (12). Vacuoles from higher plants have been isolated by osmotic lysis of protoplasts (18), treatment of protoplasts with polybases (6, 7), or centrifugation of protoplasts at high g forces against a solution of Ficoll (16).The wall-less flagellate P. malhamensis is a fresh water organism with two unequal flagellae, belonging to the order Chrysomonadina, a group whose members characteristically store oil and leucosin. Leucosin (= chrysolaminarin) is stored in a posterior vacuole, which may be so large as to almost fill the cell. Leucosin is a polysaccharide composed of beta- (1-->3)-linked d-glucose residues. It has a degree of polymerization of about 34, is water soluble, and probably has a slightly branched structure (1).

Journal Article↗
PubMed1979

Metabolism in the protozoon Allogromia laticollaris Arnold.

The foraminiferan Allogramia laticollaris, deprived of its natural food, Chlorella, incorporated and metabolized D-glucose added to culture fluid; L-glucose and inulin were not taken up. Fructose 6-phosphate and ATP were also incorporated, probably in a non-degraded form, and metabolized. Equal distribution of radioactivity of [gamma-32P]-ATP added to the medium was observed within 10 min. Intracellular glucose concentrations, and rates of respiration, glucose phosphorylation, and glycolysis were determined. Glucose utilization was stimulated with increasing concentrations of glucose, whereas respiration remained constant. The respiratory quotient increased in accordance with the higher contribution of glucose to energy supply. A. laticollaris can, then, be used for experimental studies of substrate utilization of a synthetic medium.

Adenosine Triphosphate↗
PubMed1979

The transformation of cytoplasmic microtubules into helices and paracrystals by halothane in the foraminifer Allogromia laticollaris Arnold.

After treatment of cells of the foraminifer Allogromia laticollaris Arnold (protozoa, rhizopoda) with the volatile anesthetic halothane in concentrations of more than 1 mM no cytoplasmic microtubules are demonstrable. In place of microtubules, other tubulin polymers that are helices or paracrystalline structures, with the same ultrastructure as seen after treatment with vinca alkaloids, can be identified. Below 1 mM halothane, besides loosely arranged paracrystals, single helices, microtubules and transition stages between microtubules and helices can be seen in close juxtaposition. Reasons for the transformation of microtubules and the involvement of microtubules in narcosis are discussed.

Animals↗
PubMed1979

Tubulin paracrystals in intermitotic nuclei of the foraminifer Allogromia laticollaris Arnold after treatment with vinblastine.

Electron microscopic investigations on the foraminifer Allogromia laticollaris showed that after treatment with 10(-3) M vinblastine tubulin paracrystals can be demonstrated in intermitotic nuclei. As these paracrystals are either membrane coated or lie free in the karyoplasm, and as in the perinuclear cytoplasm, membrane coated paracrystals can be demonstrated as well, it is assumed that the cytoplasmic tubulin which is composing the intranuclear division spindle can transverse the intact nuclear envelope via vesicle transport.

Animals↗