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Biomedical subjects

D S Patterson

Publications and source records attributed to D S Patterson.

At least 37 records · Page 2Linked to original sources

Border disease: a sequential study of surviving lambs and an assessment of its effect on profitability.

Pregnant Dorset horn gimmers inoculated with a non-cytopathic strain of the border disease virus produced "hairy shaker" lambs that were individually affected to a varying degree. Surviving lambs were observed for a period of up to 20 weeks when neurological signs were seen to gradually disappear. During this time live virus was isolated from a wide variety of sites in nearly all of the infected lambs. The infected animals grew at a significantly slower rate compared with the controls, particularly during the first 15 weeks. Several morphological defects are described the most consistent being the decreased weight of the central nervous system. By 20 weeks body-weights were about 20 per cent lower and carcase quality scores were reduced. An assessment is made of the economic losses attributable to BD taking into consideration carcase quality at 20 weeks and the perinatal losses.

Animals↗

The biological selenium status of livestock in Britain as indicated by sheep erythrocyte glutathione peroxidase activity.

The reliability of erythrocyte glutathione peroxidase activity as an indicator of selenium status in livestock is discussed. Based on this measurement, a survey is described of the biological selenium status of sheep on each of 329 farms in Britain. Results showed that 47 per cent of these farms were probably unable to provide grazing livestock with sufficient selenium to maintain blood levels greater than 0.075 microgram per ml. Increased selenium deficiency from the increasing use of home grown feeds as a major constituent of livestock rations may be causally related to the increase of white muscle disease and other selenium responsive diseases in Britain.

Animals↗

Tremorgenic toxins produced by soil fungi.

Penitrem A or an unknown tremorgenic toxin, "X," was produced by 10 of 60 fungal isolates obtained from a pasture involved in an outbreak in cattle and sheep resembling migram and ryegrass staggers. Tremorgenic properties of extracts containing penitrem A or toxin X were confirmed by bioassay.

Animals↗

Mycotoxins in animal feedstuffs: sensitive thin layer chromatographic detection of aflatoxin, ochratoxin A, sterigmatocystin, zearalenone, and T-2 toxin.

Improvements have been made to a previously described multi-mycotoxin method that involved a membrane cleanup step. Using 2-dimensional thin layer chromatography and appropriate solvent systems, aflatoxin B1 can be detected in mixed feedstuffs and various ingredients at levels ranging from 0.1 to 0.3 microgram/kg. Corresponding detection limits for ochratoxin A and sterigmatocystin are 5 to 20 microgram/kg and for T-2 toxin and zearalenone 20 to 200 microgram/kg.

Aflatoxins↗

Biochemical indicators of liver injury in calves with experimental fascioliasis.

Twenty calves were infected with 1000 metacercariae of Fasciola hepatica, the activities of 10 enzymes in plasma or serum were assayed and concentrations in serum of proteins, urea and bilirubin were determined. These values were compared with control data obtained from 14 uninfected calves. Aspartate aminotransferase, lactate dehydrogenase, sorbitol dehydrogenase, glutamate dehydrogenase, ornithine carbamoyl transferase and gamma-glutamyl transpeptidase activities increased in infected calves. Total serum protein increased, albumin decreased, globulin increased and the albumin/globulin ratio was decreased in infected calves. Plasma alanine aminotransferase, leucine aminopeptidase, alkaline phosphatase and cholinesterase activities and serum concentration of urea and bilirubin were unaffected. It was concluded that glutamate dehydrogenase and gamma-glutamyl transpeptidase were the most sensitive indicators of liver cell damage in fascioliasis.

Animals↗