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Biomedical subjects

D Ruano-Gil

Publications and source records attributed to D Ruano-Gil.

At least 37 records · Page 2Linked to original sources
PubMed1986

Arrangement of the sclerocorneal trabecular system in human fetuses.

By studying the eyes of 20 human fetuses 33-326 mm in length (8 to 36 weeks gestation) with the light and scanning electron microscope we show that the sclerocorneal and the uveal trabecula cannot be considered as independent structures, since embryologically and morphologically they are the sclero-corneo-iridal insertions of the longitudinal fibres of the ciliary muscle.

Cornea↗
PubMed1985

Embryonal hypermobility and articular development.

This investigation was devised to correlate development of the articular system of the embryo with variations of motility by introducing reserpine to chick embryos at a Hamburger-Hamilton stage 25-26. Administration of reserpine was followed by hypermotility or paralysis in a dose-dependent fashion. Size of joint cavities varies with motility of the limbs. Larger than normal cavities are due to excess movements, while lack of these impedes differentiation. These data are consistent with the primary role of motility in articular development.

Animals↗
PubMed1984

Atypical cristae (paracrystalline inclusions) in mitochondria of epithelial cells of the rat ureter. I. Ultrastructural features.

The cells of the basal layer of the ureter epithelium of senile rat have been studied by means of thin section electron microscopy. One of the main features of these cells was the presence of paracrystalline structures within unusually long mitochondria (megamitochondria). The detailed study of these structures showed that they were produced by the mitochondrion itself through alterations of one or two longitudinally oriented cristae. The megamitochondria were completely surrounded by membranes of rough endoplasmic reticulum, frequently showed lipid lamellae at one end, and were always situated in the vicinity of the nucleus. The possible morphogenetic mechanisms of the paracrystalline structures are discussed.

Animals↗
PubMed1984

Atypical cristae (paracrystalline inclusions) in mitochondria of epithelial cell of the rat ureter. II. Image analysis.

Long mitochondria containing paracrystalline inclusions have been detected in the epithelial cells of the basal layer of the rat ureter close to the bladder (Barastegui and Ruano-Gil, 1978). In the first part of this investigation the origin of these inclusions from alterations of the cristae has been suggested. The current report deals with an analysis by microdensitometry, optical diffraction and goniometry of the inclusions as well as with their possible architecture. A tridimensional model of the paracrystalline inclusions is proposed.

Animals↗
PubMed1984

[Development of 3 populations of embryonal bodies in monolayer culture and in suspension].

In the present investigation the "in vitro" behaviour of three morphologically different types of embryoid bodies of the teratocarcinoma OTT 6050, which were isolated by means of a Ficoll's density gradient, was analyzed. The study of the results obtained from the culture in suspension and in monolayer show that these morphologically different types represent three different stages of one evolutional cycle which tends to reproduce them as well as creating a variety of potentially more malign embryoid body.

Animals↗
PubMed1983

Experimental analysis on the variations of acid-base balance in the chick embryo.

1. The pH remains steady, 7.57. 2. The pCO2 increases gradually. We believe this is fundamental for hatching. 3. Bicarbonate and base excess increase from acidosis to alkalosis due to absorption of the egg shell. 4. The pO2 increases in stage 41 and 42 due to pulmonary respiration. 5. The pO2 drop prior to hatching due to involution of the chorioalantoid membrane and increasing requirements.

Acid-Base Equilibrium↗
PubMed1983

[Morphological study of a homogenous population of teratocarcinoma cells].

When an experimental teratocarcinoma was injected into the peritoneal cavity of isogenous 129/Sv mice, ascites developed and cell aggregates known as embryoid bodies were isolated from the ascitic fluid. Ficoll gradients have been used to seperate the embryoid bodies and their evolution in the peritoneal cavity has been followed by scanning electron microscopy.

Animals↗
PubMed1980

Deformities of the nasal septum in human foetuses.

In a seris of 50 embryos and foetuses ranging from 20 mm to 190 mm in length two foetuses of 34 and 37 mm (48 to 50 days old) were found, that presented deformities of the septum of the cartilaginous nasal capsule, representing 4% of our material. The deformities consisted of curves of the nasal septum, located to both vomeronasal nerves. We therefore think that this study is interesting because it adds a new factor, the congenital one, to the etiology of deformities of the nasal septum.

Fetus↗
PubMed1978

Influence of extrinsic factors on the development of the articular system.

In the present study, the results obtained after embryonic movements were paralyzed are described. Paralysis was produced by succinylcholine administered to chicken embryos during Hamburger-Hamilton's stages 25-29. The study of microscopic sections across all the articulations emphasize the absence of articular cavity formation. The cavities were replaced by a non-differentiated mass of mesenchymatous tissue. Regression of articular cavities and para-articular structures was noted in already formed embryos. Contrary to the opinion sustained by some authors, modifications on the articular surfaces were not produced. All of these data, reveal the importance of embryonic movements in articulation formation and conservation.

Animals↗
PubMed1978

[The first stages of the appendix development in the human embryo].

The cecoappendicular area of 14 human embryos with a length ranging from 6 to 17 mm from vertex to coccyx (28 to 48 days old; Carnegie stages 13 to 19) was studied. The appendix is not an atrophic remain of the caecum, it develops at the same time as the caecum. The first morphological anlage of the organ is observed in human embryos from 6 to 10 mm (28 to 37 days of age; Carnegie stages 13 to 16) in the form of an elevation covered with coelomic epithelium, constituted by the mesenchyme which surrounds the anlage of the caecum. In later stages, on human embryos of 11 to 16 mm (37 to 44 days of age; Carnegie stages 16 to 18), the mesoblastic anlage of the appendix is more evident, but it is not invaded by the entoblastic cells which come from the caecum on embryos of 12 to 13 mm. On embryos of about 17 mm (48 days old; Carnegie stage 19) the entoblastic anlage of the appendix has invaded the original mesoblastic anlage. The formation of the appendix by two anlage one earlier, mesoblastic, and another later, entoblastic, is similar to that of other lymphoid organs like the sack of Fabricius in birds.

Appendix↗
PubMed1976

[Obstruction and normal rechannelization of the ureter in the human embryo. Relations to stenoses and congenital ureteral valves].

Study of 5 normal human embryos from 5 to 55 mm in length from vertex to coccyx, shows that the ureteric ducts, which in the first phases of development are permeable (embryos of 5-13 mm, Streeter's Horizons XIII-XVII, age from 28 +/- 1 to 35 +/- 1 days), constantly undergo a process of obstruction and a subsequent recanalization of their lumen, which takes place when the embryos measure from 14 to 22 mm. The processes begin in the middle zone of the ureters and progress proximally and distally until they cover its entire length. These ducts, which in embryos of approximately 17 mm in length form solid cords, are totally permeable in embryos of 23 mm. These modifications are of great importance for correct interpretation of the pathogenic mechanism of congenital ureteric strictures and valves.

Humans↗
PubMed1975

Human embryo (12 mm) with mesohydronephrosis and ureterohydronephrosis.

This report describes a human embryo with a length of 12 mm (horizon XVII Streeterm 35 +/- 1 day old), in which the following malformations appear: Dilation on both sides of the Wolffian ducts, and the more caudal mesonephritic glomerules (mesohydronephrosis). Dilatation of both ureters and renal pelvis (uretero-hydronephrosis). These malformations are the result of the accumulation of liquid secreted by the mesonephros in a cloaca which is dilated, unwalled and blocked by an epithelial plug which is continuous with the cloacal membrane.

Abnormalities, Multiple↗
PubMed1975

Contribution to the study of the development of the terminal portion of the Wolffian duct and the ureter.

Ten human embryos ranging from 10 to 20 mm in vertex-coccyx length (Horizons XVI and XVII Streeter) aged between 33 +/- 1 and 39 +/- 1 days, were studied in order to interpret the mechanism which determines the displacement of the ureter from the dorsal part of the Wolffian duct toward the lateral wall of the bladder. We demonstrated that this movement occurs due to the fact that the duct common to the ureter and the Wolffian duct undergoes a process of internal rotation before it is absorbed by the urogenital sinus. This determines that the ureteral orifice enter the urogenital sinus laterally with respect to the Wolffian duct. This absorption indicates that the mucosa of the trigone is of mesoblastic origin and the remainder of the besical of endoblastic origin. The origin of the muscular apparatus of the detrusor is entirely mesoblastic.

Cell Movement↗
PubMed1975

Obstruction and normal recanalization of the ureter in the human embryo. Its relation to congenital ureteric obstruction.

In this work, after the study of 45 normal human embryos of 5-55 mm from vertex to coccyx, it is shown that the ureteric ducts, which in the first phases of development are permeable (embryos of 5-13 mm), constantly undergo a process of obstruction and posterior recanalization of their lumen, which takes place when they are from 14 to 22 mm. These processes begin in the middle zone of the ureters and progress proximally and distally until they cover its entire length, for which these ducts, which in embryos of approximately 17 mm in length form solid cords, but in the embryos of 23 mm are totally permeable. This obstructive process is found in relation to atrophy and loss of activity of the mesonephros, while that of recanalization follows the intense longitudinal growth of the ureters in this phase of development. These modifications, which the ureteric lumen normally undergoes during its embryonic development, are of great importance for correct interpretation of the pathogenic mechanism of congenital ureteric strictures and valves.

Cell Differentiation↗