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Biomedical subjects

D Roman

Publications and source records attributed to D Roman.

28 records · Page 2Linked to original sources
PubMedDate not supplied

Effects of latanoprost and GLC756, a novel dopamine D2 agonist and D1 antagonist, on cultured normal human dermal fibroblasts.

PURPOSE: Proliferation of subconjunctival fibroblasts plays a critical role in scarring and failure of glaucoma filtering surgery. Long-term topical glaucoma medications appear to increase fibroblast proliferation. In this study, the effects of topical antiglaucoma drugs latanoprost and GLC756, a novel dopamine D2 agonist and D1 antagonist, on cultured normal human dermal fibroblasts (NHDF) were examined. METHODS: The NHDF cell line was incubated with latanoprost, prostaglandin F2alpha (PGF2alpha), GLC756, or 5-fluorouracil as a positive control at concentrations of 3 and 30 microM for 6, 18, and 24 hours. Fibroblast growth was measured by 5-bromodeoxyuridine (BrdU) uptake using laser scanning cytometry. RESULTS: Latanoprost and PGF2alpha had a biphasic response on the number of cultured NHDF positively stained with BrdU. A stimulating effect on proliferation occurred early, 6 hours after incubation, and an inhibitory effect 18 to 24 hours after incubation. GLC756, in contrast, revealed only inhibitory effects on BrdU uptake 18 to 24 hours after incubation. The pattern of GLC756 was similar to that of the positive control 5-fluorouracil. CONCLUSIONS: Latanoprost seemed to have a biphasic response on the proliferation of cultured NHDF. First there was a stimulating thereafter a secondary negative modulating effect. GLC756 had a fully antiproliferative effect on the NHDF, indicating an additional potential of novel dopamine compounds for topical glaucoma medication.

Antihypertensive Agents↗
PubMedDate not supplied

Effects of antiglaucoma drugs GLC756, a novel dopamine D2 agonist and D1 antagonist, and timolol on endotoxin-induced TNF-alpha release in serum of rats.

PURPOSE: Anti-inflammatory activity of an antiglaucoma drug may be an advantage for long-term treatment of glaucoma since it may reduce the risk of treatment-related inflammatory processes in outer compartments of the eye and probably also prevent or delay progression of glaucomatous retinal neurodegeneration. In this study, the effect of GLC756, a novel mixed dopamine D 2 receptor agonist and dopamine D 1 receptor antagonist, and timolol on endo-toxin-induced cytokine tumor necrosis factor-alpha (TNF-alpha) release in serum was examined. METHODS: For endotoxin-induced TNF-alpha release, 8-week-old Lewis rats were intravenously injected with 160 microg lipopolysaccharide (LPS) from Salmonella typhimurium. GLC756, timolol, or betamethasone were either systemically (1 mg/kg SC for 5 days) or topically (0.4%, 0.5%, and 0.1%, respectively, 20 microL eye drops given 16 times over 48 hours in left and right eye) administered. TNF-alpha was measured in serum 2 and 48 hours after LPS induction. RESULTS: A marked TNF-alpha increase in serum was found 2 hours after LPS induction. Administration of GLC756 and betamethasone, systemically and topically, decreased TNF-alpha release. However, due to large scattering of mean values only the effect of systemically administered GLC756 was statistically significant. In contrast, timolol increased TNF-alpha values stronger than LPS alone. CONCLUSIONS: The significant suppression of LPS-induced TNF-alpha increase by GLC756 suggests an additional anti-inflammatory potential of the dopaminergic compound in the treatment of glaucoma.

Animals↗
PubMedDate not supplied

[The incidence of HBsAg in different population groups of Moldavia in 1991 compared to 1973].

AgHBs incidence in various population groups from the Iaşi region was relatively high in 1991, ranging from 3.8% in blood donors to 75.0% in patients with severe acute virus hepatitis. As compared to the incidence values recorded in 1973, 18 years ago, in the population of the same territory, some mutations were observed, the most important being the more then threefold reduction of the AgHBs incidence in blood donors. In subjects with liver diseases and in risk groups, the frequency of AgHBs carriers maintains at a high level.

Adult↗
PubMedDate not supplied

[Incidence of HBsAg among different population groups in Moldavia in 1991 compared with 1973].

AgHBs incidence in various population groups from the Iassy region was relatively high in 1991, ranging from 3.8% in blood donors to 75.0% in patients with severe acute virus hepatitis. As compared to the incidence values recorded in 1973, 18 years ago, in the population of the same territory, some mutations were observed, the most important being the more then threefold reduction of the AgHBs incidence in blood donors. In subjects with liver diseases and in risk groups, the frequency of AgHBs carriers maintains at a high level.

Adult↗