[The corneal replica technic in animals].
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Biomedical subjects
Publications and source records attributed to D Rigal.
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Immune regulatory effects of histamine are now well established. We have previously demonstrated that human T lymphocytes having histamine receptors, produce a soluble factor inhibiting the production of LIF. In this present paper we have shown that production and function of this factor is not species specific. Indeed the leukocyte inhibiting factor-production inhibitor (LIF-PI) produced by human, guinea pig or dog lymphocytes inhibits the production of LIF by human, guinea pig and murine lymphocytes to a similar extent. This property allows for easier testing of LIF-PI particularly from human subjects.
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Canine T lymphocytes were detected, using fluorescent peanut agglutinin (PNA) as a marker. Using a fluorescent technique and cytofluorometry, 70 +/- 11% and 72.4%, respectively, of peripheral blood lymphocytes were bound to PNA. Of thymocytes, 97 +/- 4.5% were detected by fluorescent PNA, but less than 1% were detected for lymphocytes from bone marrow. The T-lymphocyte depletion and enrichment indicated that PNA was bound to lymphocytes recognized by anti-T-lymphocyte heterologous serum. A T-lymphocyte deficiency was detected among 8 dogs with a lupus-like syndrome.
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A 5,9 year old girl presented a severe and acute auto-immune hemolytic anemia, unresponsive to steroids, treated by 10 plasma exchanges, immunosuppressive agents and splenectomy. Despite an anticoagulant treatment, occurrence of multiple thrombosis. Unusual evolution of Mycoplasma serological tests.
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The first case is provided by a french women (KK, husband k/k) who delivered a first healthy full term infant in 1970 and received one blood unit at this time. In 1972 and 1975, his second and fourth pregnancies ended by spontaneous abortion. In 1974 and 1976, she delivered stillborn infants with hydrops fetalis during the sixth month. In serum, anti-k antibody reacted by the indirect antiglobulin test with a titer of 4 096. In the second case, french women delivered a first full term female infant who had a strongly positive direct antiglobulin test. The mother had received one blood unit ten years before during nephrectomy. Her red blood cell were KK and her husband was kk. She had a anti-Cellano in serum reactive in the indirect antiglobulin test with a titer of 16. The infant developed a mild hyperbilirubinemia and recovered without treatment these two cases confirm the variable effect of maternal anti-k antibody evidenced by few other published cases.
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