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Biomedical subjects

D R Boone

Publications and source records attributed to D R Boone.

18 recordsLinked to original sources
PubMed1991

Spouse attitudes toward the person with aphasia.

This investigation evaluated the attitudes of individuals towards their aphasic spouse. Using modified Q-methodology, 15 spouses of fluent aphasic patients, 15 spouses of nonfluent aphasic patients, and 30 matched controls completed a 70-item Q-sort. The spouses of nonfluent aphasic patients had a significantly greater number of negative attitudes toward their spouses than the spouses of fluent aphasic patients. The spouses of patients in both aphasia groups had a significantly greater number of negative attitudes toward their spouses than the matched controls. The most common attitudes of spouses of patients in both aphasic groups divided into six factors: compliance, desirability, egocentricity, independence, maturity, and sociability.

Aphasia, Broca↗
PubMed1991

Isolation and characterization of a dimethyl sulfide-degrading methanogen, Methanolobus siciliae HI350, from an oil well, characterization of M. siciliae T4/MT, and emendation of M. siciliae.

We isolated strain HI350 from a gas and oil well in the Gulf of Mexico, characterized it, and found that it is closely related to Methanolobus siciliae T4/MT (T = type strain), which we also characterized. The previously published characterization of the type strain of M. siciliae was limited to the optimum temperature for growth, and our characterization suggested the species description given below. Cells are irregular, nonmotile, coccoid, and 1.5 to 3 microns in diameter. The catabolic substrates used include methanol, trimethylamine, and dimethyl sulfide, but not H2-CO2, formate, or acetate. Growth is fastest in the presence of 0.4 to 0.6 M Na+, in the presence of 60 to 200 mM Mg2+, at pH 6.5 to 6.8, and at 40 degrees C. Growth on trimethylamine is stimulated by yeast extract. An electrophoretic analysis confirmed that strain HI350 is closely related to strain T4/MT and indicated that major changes in the intracellular proteins of M. siciliae HI350 occur when the growth substrate is switched between dimethyl sulfide and trimethylamine.

Base Composition↗
PubMed1990

Speech rate and syntactic complexity effects on the auditory comprehension of Alzheimer patients.

The purpose of this investigation was to examine the effects of speech rate and syntactic complexity on the auditory language comprehension of individuals with presumptive Alzheimer's disease, compared to healthy elderly controls. Three presentation rates and command statements of increasing syntactic complexity were used. Although rate of presentation did not significantly affect comprehension in either group, both groups demonstrated increased difficulty with stimuli of greater syntactic complexity, with Alzheimer's patients performing significantly poorer at all levels.

Aged↗
PubMed1990

Isolation and characterization of disaggregatase from Methanosarcina mazei LYC.

At certain stages in its growth cycle, Methanosarcina mazei produces an enzyme (disaggregatase) that causes aggregates to separate into single cells. M. mazei S-6 and LYC both produce this enzymatic activity, although the specificities of activities differ. The disaggregatase of M. mazei S-6 had little effect on strain LYC cells, but the disaggregatase of M. mazei LYC disaggregated both strain LYC and strain S-6 cells. The disaggregatase of M. mazei LYC was purified by column chromatography, and it apparently consisted of two similar subunits with a combined molecular size of about 180,000 Da. Strain S-6 culture supernatants contained 14 U of activity per liter when activity was measured as uronic acids released from purified cell wall material. When the activity was quantified as the release of uronic acids from boiled M. mazei S-6 cells, the highest activity was found at pH 4.7 and at 35 degrees C.

Amino Acid Sequence↗
PubMed1990

Detection of the osmoregulator betaine in methanogens.

Trimethyl glycine (glycine betaine) was detected by 13C nuclear magnetic resonance spectroscopy at high intracellular concentrations in several methanogens (Methanogenium cariaci, "Methanogenium anulus" AN9, Methanohalophilus zhilinae, Methanohalophilus mahii, and Methanococcus voltae) grown on marine media containing yeast extract. 13C labeling studies with Methanogenium cariaci suggested that the betaine which accumulated inside the cells was not synthesized de novo but was transported in from the medium. Proof of such a transport system was provided by growing Methanogenium cariaci on yeast-free medium supplemented with betaine. Under these conditions, betaine was the dominant osmoregulator.

Betaine↗
PubMed1990

Occurrence of beta-glutamate, a novel osmolyte, in marine methanogenic bacteria.

The unusual compound beta-aminoglutaric acid (beta-glutamate) has been identified by 13C nuclear magnetic resonance spectroscopy in soluble extracts of marine methanogenic bacteria. We examined several methanogen species representing nine genera and found that beta-glutamate occurred in methanococci and two methanogenium strains (Methanogenium cariaci JR1 and "Methanogenium anulus" AN9). The presence of this compound in the methanococci examined was further restricted to thermophilic members of the genus Methanococcus, including Methanococcus thermolithotrophicus strains, Methanococcus jannaschii, and "Methanococcus igneus." The two Methanogenium strains examined were mesophiles. Studies using Methanococcus thermolithotrophicus showed that levels of beta-glutamate in cells of that species were not affected by variation in growth temperature (40 to 65 degrees C), NH4+ (2 to 80 mM), Mg2+ (10 to 50 mM), or K+ (2 to 10 mM) in the medium. In contrast, soluble pools of beta-glutamate and L-alpha-glutamate (the other major free amino acid in all the methanococci) were proportional to NaCl levels in the growth medium. This dependence of beta-glutamate and L-alpha-glutamate concentrations on salt levels in the medium suggests that they function as osmolytes in these cells.

Euryarchaeota↗
PubMed1989

Differentiating Alzheimer's patients from the normal elderly and stroke patients with aphasia.

The performance of individuals with mild and moderate Alzheimer's disease (AD), normal age-matched elderly individuals, and stroke patients with fluent and nonfluent aphasia were compared on a group of neuropsychological tasks. The unique performance profiles associated with each subject group are discussed, and the best tasks for intergroup differentiation specified. Whereas the tasks employed were efficacious for discriminating early- and middle-stage AD patients from normal subjects and aphasic stroke patients, and early- from middle-stage AD patients, they were not efficacious for subtyping aphasia patients according to fluency. Generally, memory measures were best for intergroup differentiation.

Aged↗
PubMed1976

Writing in aphasia rehabilitation: cursive vs manuscript.

Reading and writing performance was observed in 30 adult aphasic patients to determine whether there was a significant difference when stimuli and manual responses were varied in the written form: cursive versus manuscript. Patients were asked to read aloud 10 words written cursively and 10 words written in manuscript form. They were then asked to write on dictation 10 words responses using cursive writing and 10 words using manuscript writing. Number of words correctly read, number of words correctly written, and number of letters correctly written in the proper sequence were tallied for both cursive and manuscript writing tasks for each patient. Results indicated no significant difference in correct response between cursive and manuscript writing style for these aphasic patients as a group; however, it was noted that individual patients varied widely in their success using one writing form over the other. It appeared that since neither writing form showed better facilitation of performance, the writing style used should be determined according to the individual patient's own preference and best performance.

Adult↗
PubMed1976

ASHA: Why?

Explore the source record for details and available documents.

Health Occupations↗
PubMedDate not supplied

Adductor spastic dysphonia: heterogeneity of physiologic and phonatory characteristics.

Physiologic and phonatory characteristics of 23 subjects with adductor spastic dysphonia were studied, including examination of the laryngeal appearance by fiberoptic nasoendoscopy, neurologic examination, and measurement of phonatory airflow and speaking fundamental frequency. These characteristics displayed considerable heterogeneity among the subjects investigated. Three patterns of laryngeal appearance during phonation were observed: vocal fold adduction, associated ventricular fold constriction, and approximation of the laryngeal inlet. Eight of 13 subjects demonstrated some neurologic abnormality. For the majority of subjects, the airflow demonstrated marked variability during sustained phonation; for different subjects, this variability was observed to be associated with either an oscillatory or irregular airflow pattern. The modal speaking fundamental frequency for the women and men with adductor spastic dysphonia was not significantly different from that for age- and sex-matched controls.

Adult↗