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Biomedical subjects

D Paul

Publications and source records attributed to D Paul.

At least 235 records · Page 13Linked to original sources
PubMed1972

Stimulation of DNA and protein synthesis in fetal-rat liver cells by serum from partially hepatectomized rats.

Primary cultures of differentiated liver cells from fetal rats respond to serum from partially hepatectomized rats by an increased uptake of [(3)H]thymidine and [(3)H]leucine as compared with cultures incubated with normal rat serum. These findings are discussed in relation to the mechanism of induction of DNA synthesis and growth in liver cells during liver regeneration.

Animals↗
PubMed1972

Studies on primary cultures of differentiated fetal liver cells.

A method for culturing non- or slowly growing, differentiated fetal rat liver cells is described. It involves the use of collagenase as a digesting agent and of a selective medium deficient in arginine which suppresses the growth of nonparenchymal liver cells. Evidence is presented that surviving cells (a) retain liver-specific urea cycle functions measured by their capacity to transform ornithine into arginine, (b) synthesize DNA in glucose-deficient medium, and (c) synthesize and secrete albumin. This primary cell culture responds to partially hepatectomized rat serum and may be an appropriate assay system for the study of mechanisms which regulate liver regeneration.

Animals↗