Biomedical subjects
D O'Brien
Publications and source records attributed to D O'Brien.
Comparison of the safety, vaccine virus shedding, and immunogenicity of influenza virus vaccine, trivalent, types A and B, live cold-adapted, administered to human immunodeficiency virus (HIV)-infected and non-HIV-infected adults.
Fifty-seven human immunodeficiency virus (HIV)-infected (CDC class A1-2) and 54 non-HIV-infected adults, not prescreened for influenza susceptibility, were randomized to receive trivalent live attenuated influenza vaccine (LAIV) or placebo intranasally. LAIV was safe and well tolerated with no serious adverse events attributable to vaccine. Reactogenicity rates were similar in LAIV and placebo recipients except that runny nose/nasal congestion was significantly more common in LAIV recipients regardless of HIV status. No prolonged shedding of LAIV was observed in HIV-infected participants. HIV RNA levels were not increased and CD4 counts were not decreased in HIV-infected LAIV recipients compared with placebo recipients after immunization. Shedding of LAIV and increases in antibody titers were infrequent, consistent with prior experience in unscreened adults. The data suggest that inadvertent vaccination with LAIV in relatively asymptomatic HIV-infected adults would not be associated with frequent significant adverse events.
The use of orbital morphine for postoperative analgesia in pterygium surgery.
A prospective double-blind study compared the analgesic effectiveness of peribulbar lignocaine with peribulbar morphine and lignocaine for postoperative analgesia in pterygium surgery. Twenty patients were randomly divided to receive a peribulbar injection preoperatively of either 1% lignocaine 2 ml or 1% lignocaine 1.6 ml and 4 mg morphine. Effects on pain at injection and pain at 24 hours and 48 hours postoperatively were measured with a visual analog pain scale. Effects of the injections on sedation, pupil size and unwanted side-effects were also recorded. The groups were comparable for age, weight and surgical technique. There was a significantly lower pain score at 24 hours after operation in the morphine group (P = 0.035). There were no significant differences in sedation or side-effects between the groups. The physiological effects of morphine on the eye are reviewed. The study suggests that orbital morphine may be an effective and safe form of analgesia for corneal surgery and further investigation is warranted.
Re-engineering an Australian emergency department: can we measure success?
In 1996, in response to perceived deficiencies of the Emergency Department, Sir Charles Gairdner Hospital made emergency medicine a key strategic initiative. Major staffing and functional changes occurred as a result, including creation of the first Chair in Emergency Medicine in Australasia. We present a before and after study, using a range of measured variables, including the accepted Australian Council on Healthcare Standards emergency medicine clinical indicators. Clinically, there were great improvements in waiting times, time to thrombolysis in acute myocardial infarction, complaint rate, and misdiagnosed fracture rate. Increased throughput of short stay patients in a re-opened observation ward greatly shortened average length of stay for patients with a range of acute conditions. Data also indicated significant improvements in teaching and research. We conclude that with firm commitment from hospital management, re-engineering an emergency department can be shown to improve the quality-of-care.
Essentials: going online.
The moment has arrived for you to go online! This article provides an overview of online access and Internet services. Resources are suggested and hints are offered to get the novice started on the information highway. Enjoy your journey!
Computer and information technology: hardware.
Computers open the door to an ever-expanding arena of knowledge and technology. Most nurses practicing in perianesthesia setting were educated before the computer era, and many fear computers and the associated technology. Frequently, the greatest difficulty is finding the resources and knowing what questions to ask. The following is the first in a series of articles on computers and information technology. This article discusses computer hardware to get the novice started or the experienced user upgraded to access new technologies and the Internet. Future articles will discuss start up and usual software applications, getting up to speed on the information superhighway, and other technologies that will broaden our knowledge and expand our personal and professional world.
Computer and information technology: software.
You have that new computer and you are ready to start computing, right? Not quite. There is another essential to get started--software. This article focuses on software necessary and nice for your computer, and resources that can help you in your quest for creating the best possible and easiest to use computer system. Once you have gotten everything installed and functioning, you will be ready to take the next step--going online. In the next issue of the Journal we will get you online and traveling on the information superhighway. Happy computing!
Toxic and metabolic causes of seizures.
Seizures are a relatively nonspecific response of the cerebral cortex to insult and can be caused by a variety of toxic or metabolic diseases. Seizure in response to extracranial disease are called reactive seizures. Toxins cause seizures by altering the balance of excitation and inhibition in the nervous system or interfering with energy metabolism. Many therapeutic or illicit drugs will cause seizures through similar mechanisms. Metabolic diseases can cause seizures by interfering with energy metabolism, changing osmolality, or producing endogenous toxins. In addition, metabolic disease may alter the pharmacokinetics of antiepileptic drugs or drugs that have the potential to cause seizures. While seizures represent a serious sign for many of these conditions, reactive epilepsy always carries the possibility that the seizures may be eliminated if the underlying cause is detected and corrected.
The ventral motoneurone axon bundle in the CNS--a cordone system?
In the developing CNS neighbouring structures are commonly separated by transient barriers termed cordones, some of which coincide with glial elements. Where ventral motoneuron axons cross the spinal white matter as intramedullary bundles to reach the CNS-PNS transitional zone they are surrounded from early development by a glial sleeve resembling a cordone. This becomes better developed with age and, like some cordones, persists into adult life. This could provide a radial conduit which might underlie the capacity of central segments of mature ventral motoneurone axons to regenerate. It may also provide a pathway for glial migration from the central cord to more superficial levels, including the transitional zone, where they help form the CNS-PNS barrier. Axons in the intramedullary bundle and in the surrounding ventral white column mature at different rates. Glial sleeve cells of the intramedullary bundles are apposed to both. Morphometric analysis of the axon-glial relationships of the two populations indicates that glial development proceeds at a different rate in relation to each axon class and that this is influenced by the degree of axonal maturation, which may in turn be related to target contact. Furthermore, early axon glial relationships differ between the two populations. For ventral motoneurone axons these take place in two stages: firstly, glial segregation of axons (resembling that in the PNS) and secondly, oligodendrocytic contact and ensheathment, which leads on to myelination. Axon-glial relationships in the ventral white column begin with the second of these events, as is more typical of early CNS myelination in general.
Evaluation of PCR for diagnosis of melioidosis.
Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inappropriate therapy resulting from a positive result by PCR have led us to forgo the advantage of rapid PCR diagnosis for melioidosis until a better system is validated.
Perceived need for education vs. actual knowledge of pressure ulcer care in a hospital nursing staff.
The relationship between perceived need for further education in wound care issues and actual knowledge of pressure ulcer care was examined among 86 professional and nonprofessional nursing staff members of a community urban hospital. A weak correlation between perceived need for further education and actual wound care knowledge base was found. Participants were poorly informed in several basic aspects of pressure ulcer care while generally desiring more wound care education. These results are discussed in light of future educational needs vis-a-vis current "re-engineering" efforts in nursing personnel.
A BsII PCR/RFLP in the renin binding protein (RnBP) gene on canine chromosome X.
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Regional anesthesia of the infraorbital and inferior alveolar nerves during noninvasive tooth pulp stimulation in halothane-anesthetized dogs.
OBJECTIVE: To document that regional anesthesia of the infraorbital and inferior alveolar nerves would abolish reflex-evoked muscle action potentials (REMP) in the digastricus muscle during noninvasive stimulation of tooth pulp in halothane-anesthetized dogs. DESIGN: Prospective study. ANIMALS: 9 healthy female dogs between 2 and 6 years old. PROCEDURE: Dogs were anesthetized using halothane. An alligator clip anodal electrode was attached to the tooth to be stimulated, and a platinum needle cathodal electrode was inserted in adjacent gingival mucosa. The cathodal and anodal electrodes were moved to the left upper and lower canine, fourth premolar, and first molar teeth for sequential stimulation. Baseline recording of REMP was made for each tooth. Catheters were inserted percutaneously in the infraorbital and mandibular canals. Saline (0.9% NaCl) solution was injected at each catheterized site in 3 control dogs, and chloroprocaine hydrochloride was injected at each catheterized site in 6 test dogs. Each tooth was stimulated every 10 minutes for 90 minutes (test dogs) or every 10 minutes for 30 minutes and at 90 minutes (control dogs), and REMP was recorded. RESULTS: REMP was abolished within 10 minutes in all test dogs, except during stimulation of the lower first molar in 1 dog. In 4 dogs, duration of blockade was less than 90 minutes. The REMP was not restored within 90 minutes for the upper teeth in 1 dog and within 2 hours for all teeth in another dog. At 24 hours, REMP was restored for all teeth except the lower left canine in 1 dog. The REMP was restored for the lower left canine in that dog at 96 hours. The REMP was not abolished at any time in control dogs. CLINICAL IMPLICATIONS: Regional anesthesia of the infraorbital and inferior alveolar nerves may effectively provide analgesia for dental procedures in dogs.
Differential induction of DNA-binding activities following CD19 cross-linking in human B lineage cells.
The B cell-specific cell surface molecule CD19 is expressed at all stages of B cell development, including normal plasma cells, and mediates signal transduction via interaction with cytoplasmic effector proteins. Cross-linking CD19 on early human B lineage cells induces the formation of a CD19/Vav/phosphatidylinositol-3 kinase complex, tyrosine phosphorylation of CD19 and Vav, and activation of the Ras pathway. To further explore the ramifications of CD19 signaling, the current study examined whether phosphorylation of Elk-1, activation of activator protein-1 (AP-1), or activation of nuclear factor-kappaB (NF-kappaB) transcription factors occurred following CD19 cross-linking. The cells used were the BLIN-1 pre-B cell line expressing low levels of cell surface mu heavy chain associated with surrogate light chain and the 1E8 immature B cell line expressing cell surface mu/kappa. Lysates from CD19 cross-linked 1E8 cells induced robust phosphorylation of an Elk-1 fusion protein in vitro, whereas no phosphorylation of Elk-1 fusion protein occurred using lysates from CD19 cross-linked BLIN-1 cells. An electrophoretic mobility shift assay employing AP-1 and NF-kappaB consensus oligonucleotides was used to demonstrate that AP-1 -binding activity increased, while constitutive NF-kappaB-binding activity was not enhanced, following 2 h of CD19 cross-linking in 1E8 cells. Supershift experiments revealed that JunD and c-Fos proteins mediated anti-CD19 induced AP-1-binding activity in 1E8 cells. In contrast, CD19 cross-linking in BLIN-1 cells resulted in the induction of NF-kappaB, but had no apparent effect on AP-1-binding activity. These data suggest that CD19-mediated signal transduction activates different transcription factors at juxtaposed stages of B cell development that may culminate in the activation or suppression of distinct sets of genes.
Delayed traumatic cerebral aneurysm after brain injury.
Traumatic aneurysms (TAs) are an unusual etiology for late neurological deterioration after traumatic brain injury (TBI) and represent less than 1% of all cerebral aneurysms. TAs most often are diagnosed acutely but may be delayed in presentation. To increase awareness of this serious but treatable condition when diagnosed early, we report a delayed TA after a motor vehicle accident. The patient experienced a seizure on day 46 postinjury while in rehabilitation and demonstrated persistent lethargy and hemiparesis. Neuroimaging revealed a large, ruptured left pericallosal artery TA, which was surgically clipped. The patient completed his rehabilitation course and was eventually discharged home with family. Among TBIs, TAs are associated with penetrating injuries and skull base or anterior cranial fossa fractures. Associated mortality is high, especially if rupture has occurred. Although TAs are rare, the clinician should be vigilant in the at-risk patient.
Impact of an education program about domestic violence on nurses and doctors in an Australian emergency department.
OBJECTIVE: To increase the knowledge of nurses and doctors in an emergency department about the topic of domestic violence; to change any negative practices and attitudes toward victims; to increase knowledge of the referral processes for psychosocial aspects of domestic violence; and to increase knowledge of community resources for domestic violence victims. METHODS: Identical knowledge, attitude, and practice surveys were conducted with nurses and doctors in an emergency department before and after an educational intervention program about domestic violence. A matched-pair analysis of those respondents who answered both pretest and posttest surveys was conducted to measure the impact of the program on nurses and doctors. RESULTS: Nurses and doctors had a reasonable knowledge of the topic of domestic violence before the education program (correct answers: nurses, 61.6%; doctors, 63.4%). However, the program had a positive impact on their knowledge (correct answers posttest: nurses, 71.5%; doctors, 72.4%), with more significant changes for nurses than for doctors. The program affected both nurses' and doctors' attitudes (of 10 positive attitudinal statements: pretest, 7.9; posttest, 8.6). On both the pretest and posttest, nurses and doctors did not subscribe to a number of the myths about domestic violence that have been described in the domestic violence literature. These findings should be treated with caution because of the low response rates to the surveys from doctors (28.0%, n = 20) and nurses (53.0%, n = 48). DISCUSSION: Further research is needed into the beliefs and practices of nurses and doctors about domestic violence. The impact of this education program highlights the necessity for introducing training programs for health professionals on domestic violence problems.