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Biomedical subjects

D Nathans

Publications and source records attributed to D Nathans.

At least 109 records · Page 6Linked to original sources
PubMed1972

Bidirectional replication of Simian Virus 40 DNA.

SV40 (Simian Virus 40) DNA was pulse-labeled with [(3)H]thymidine in infected monkey cells, and the distribution of label within newly completed molecules was determined by analysis of specific fragments produced by restriction endonuclease from Hemophilus influenzae. From these data, an order of synthesis or temporal order of the fragments was deduced. Comparison of the temporal order with the physical order of the fragments in the SV40 DNA molecule indicates a correspondence in these orders for two separate groups of fragments. From an analysis of the results, we conclude that SV40 DNA replication begins at a specific site and proceeds bidirectionally, terminating about halfway around the circular molecule from the initiation point.

Animals↗
PubMed1971

Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.

A bacterial restriction endonuclease has been used to produce specific fragments of SV40 DNA. Digestion of DNA from plaque-purified stocks of SV40 with the restriction endonuclease from Hemophilus influenzae gave 11 fragments resolvable by polyacrylamide gel electrophoresis, eight of which were equimolar with the original DNA. The fragments ranged from about 6.5 x 10(5) to 7.4 x 10(4) daltons, as determined by electron microscopy, DNA content, or electrophoretic mobility.

Acrylamides↗