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Biomedical subjects

D N Pandey

Publications and source records attributed to D N Pandey.

30 records · Page 2Linked to original sources
PubMedDate not supplied

Some observations on human semen analysis.

Semen analysis of 66 unmarried medical students in the age group of 17-21 years was carried out. A higher liquefaction time pH, motility, lower sperm count and abnormal forms were observed compared to reported values. Liquefaction time, pH and sperm count was found significantly different in non-vegetarians and vegetarians, perhaps due to difference in their dietary proteins.

Adolescent↗
PubMedDate not supplied

Urinary aspartate transaminase (AST) activity and detection of its inhibitors in normal urine.

Urinary aspartate-transaminase activity in the whole urine was found to be mean +/- S.D. = 8.46 +/- 0.69 l.U/l when measured immediately after urine collection. About 50% loss in enzyme activity was observed after 18 hours dialysis. An overall 176% increase in enzyme activity followed by Sephadex G-25 (fine) whole urine fractionation and a highly significant (P less than .001) partial inhibition by earlier Sephadex fractions and maximum inhibition by earlier Sephadex fractions and maximum inhibition of enzyme by fraction 7 have suggested the presence of both high and low molecular weight urinary inhibitors of aspartate-transaminase. Urea and ammonia presence and inhibitor activity in fraction 6 to 8 bear a close parallelism; both the substances produced 31% inhibition of partially purified goat liver GOT at concentrations approximating normal human urine. Therefore, low enzyme activity and its substantial loss in the whole urine and during dialysis may be due to the concomitant inhibitory effects of urea, ammonia and unidentified nature of high molecular weight substance(s). The present method may be effective in separating inhibitors and overcoming the disadvantages of dialysis in determining true urinary aspartate-transaminase activity.

Adult↗
PubMedDate not supplied

Study of the intake of fluid, output and titratable acidity of urine in medical students.

183 students of 17-22 years age group were studied in respect of fluid intake, urine output and urine titratable acidity under normal conditions for two consecutive days. The study was performed in early winter in eastern part of U.P. Male students were observed to have higher fluid intake and urine output as compared to female. Average fluid intake and urinary output in male students were observed to be 2860 S.D. +/- 780 ml and 1860 S.D. +/- 880 ml respectively while corresponding readings for female students were 1980 S.D. +/- 390 ml and 1550 S.D. +/- 840 ml. Titratable acidity was observed to be high with a mean of 47.46 meg S.D. +/- 16.2 meq as compared to commonly accepted average range of 25-35 meq. Nonvegetarian subjects were found to have significnatly higher titratable acidity. Fluid intake and urine output did not have any significant effect on titratable acidity of urine in both the sexes. Need for further studies on this subject has been stressed.

Acids↗