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Biomedical subjects

D N Buell

Publications and source records attributed to D N Buell.

31 records · Page 2Linked to original sources
PubMed1973

Separation of human lymphoid cells into G 1 , S, and G 2 cell cycle populations by use of a velocity sedimentation technique.

Human lymphoid tissue culture cells can be separated according to cell size and corresponding cell cycle phase with a velocity sedimentation centrifugation method employing a continuous 5-20% wt/wt Ficoll gradient. A 7-fold increase in streaming limit was achieved by placing a buffer zone of isosmolar 5% Ficoll on top of the gradient before application of the cell load. The various pooled populations of cells from upper, middle, and lower areas of the gradient were characterized using autoradiographic, TCA-precipitable (3)H]thymidine incorporation, and Fuelgen microspectrophotometric methods. The upper range of the gradient contains cells in the G(1) cell cycle phase; the lower range, cells in the G(2) phase; cells found in the middle of the gradient belong largely to the S phase of the cell cycle. These gradient-separated cell pools contained relatively little contamination with cells from other phases of the cell cycle and, when explanted from the gradient into fresh growth media, showed growth patterns characteristic of synchronized cell populations. This system of cell separation provides a useful tool for investigating the relationship of the cell cycle to surface membrane and metabolic characteristics in human lymphoid cell culture systems.

Autoradiography↗
PubMed1972

Water-soluble insulin receptors from human lymphocytes.

Specific insulin receptors from human lymphocytes in culture have been prepared in aqueous solution without use of detergents or related compounds. Receptors prepared in this fashion exhibit characteristics identical to those reported in intact cells.

Adrenocorticotropic Hormone↗
PubMed1969

Limited periods of gene expression in immunoglobulin-synthesizing cells.

In synchronized human lymphoid cell lines, production of immunoglobulins G and M is greatest during the late Gl and S phases of the cell cycle. Little immunoglobulin appears immediately before, during, and immediately after mitosis. The results indicate that transcription of immunoglobulin genes takes place during a limited part of the mitotic cycle.

Burkitt Lymphoma↗
PubMed1969

Biosynthetic and structural studies of a heavy chain disease protein.

A new heavy chain disease protein ((gamma)HCD-JM) has been characterized by antigenic and structural criteria. The protein belongs to the IgG3-subclass and is closely related to Fc-fragment of G3-immunoglobulins. The predominant N-terminal amino acid of this protein is glutamic acid in the uncyclized form, and that of another (gamma)HCD is glycine. Studies of the N-terminal peptides indicate that the N-terminal portion of the (gamma)3-heavy polypeptide chain is absent from the (gamma)HCD-JM. These findings rule out a process of normal heavy chain initiation and a large deletion of the Fd region as being responsible for these two heavy chain disease proteins. The (gamma)HCD-JM is a secretory product of cells from bone marrow as shown by studies of in vitro incorporation of amino acids-(14)C. Bone marrow and lymph node have a population of lymphoplasmacytic cells which by immunofluorescence contain (gamma)-heavy chain antigens in the absence of light chain antigens.

Amino Acids↗
PubMedDate not supplied

Fluconazole versus amphotericin B in the treatment of esophageal candidiasis in cancer patients.

Thirty-one patients with malignancy and endoscopy-confirmed symptomatic esophageal candidiasis were randomly assigned to receive oral fluconazole, 200 mg once daily, or intravenous amphotericin B, 0.3 mg/kg, over 1-4 weeks. Clinical efficacy was determined weekly and follow-up esophageal endoscopy with biopsy and culture performed whenever possible. Both agents produced rapid resolution of symptoms, especially dysphagia and odynophagia. All 13 evaluable patients in the fluconazole group and 10/12 treated with amphotericin had clinical improvement. Endoscopy showed eradication of the fungus in all 11 patients undergoing repeat endoscopy, with cure or improvement of esophagitis. The incidence of adverse effects attributable to treatment was higher in the amphotericin group. The efficacy of oral fluconazole in esophageal candidiasis appears to be equivalent to that of amphotericin, with fewer adverse reactions.

Adolescent↗
PubMedDate not supplied

Clinical Oncology Program: a community cancer control model.

The majority of cancer patients are diagnosed and treated in their local communities, making it imperative to upgrade cancer management at the community level. The Clinical Oncology Program has demonstrated that sophisticated cancer control programs are possible in a community setting. Moreover, if "quality of survival" data collection is included in such programs, valuable information emerges that can further improve cancer management in the community.

Hospitals, Community↗