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Biomedical subjects

D N Baron

Publications and source records attributed to D N Baron.

106 records · Page 6Linked to original sources
PubMed1968

Preparation of isolated multiple forms of rabbit brain aldolase.

1. A method is described for partial purification of the total aldolase of rabbit brain by ammonium sulphate precipitation followed by fractionation of the aldolase into five electrophoretically distinct forms. 2. The method allows a laboratory-scale preparation of the five aldolases. 3. With the exception of band 3 the fructose 1,6-diphosphate/fructose 1-phosphate activity ratios of the isolated multiple forms show a continuous increase from isoenzyme 1 to isoenzyme 5; this evidence supports the four-sub-unit model.

Animals↗
PubMed1965

Dehydrogenation of androsterone by purified 3-alpha-hydroxy steroid-dependent nicotinamide-adenine dinucleotide (phosphate)-transhydrogenating enzyme of rat liver.

1. An enzyme from rat liver, catalysing 3alpha-hydroxy steroid-dependent NAD(P) transhydrogenation and NAD-linked and NADP-linked dehydrogenation of 3alpha-hydroxy steroids, has been purified 100-fold by chromatography on DEAE-cellulose and calcium phosphate gel. 2. No separation of these activities into different protein fractions has been achieved. 3. The properties of the enzyme in catalysing NAD-linked and NADP-linked dehydrogenation have been compared, with androsterone as substrate. Differences were found in pH optima, affinity for coenzyme and steroid, equilibrium constants and effects of salts. 4. NAD-linked dehydrogenation is inhibited by NADPH(2) but is protected from this inhibition by chloride, which alone is itself an inhibitor. 5. The relevance of these findings to the problem of the number of enzymes involved in catalysis of 3alpha-hydroxy steroid-dependent transhydrogenation is discussed.

Androsterone↗