Biomedical subjects
Publications and source records attributed to D Moyer.
The closure of surgical wounds can be improved by applying some basic techniques for suturing and for correcting suture errors. These techniques include 1) the locking stitch, 2) the pulley stitch for closing large gaping defects, and 3) a means of welding polyfilament synthetic polyamide suture and polypropylene suture.
We have developed the yeast Kluyveromyces lactis as a host organism for the production of the milk-clotting enzyme chymosin. In contrast to Saccharomyces cerevisiae, we found that this yeast is capable of the synthesis and secretion of fully active prochymosin. Various signal sequences could be used to efficiently direct the secretion of prochymosin in Kluyveromyces, but not in S. cerevisiae. We conclude that the efficient synthetic and secretory capacity of this heterologous protein is a property of the yeast Kluyveromyces. These results have led to the development of a large scale production process for chymosin.
Dating of maturity of the endometrium by histologic examination was correlated with four methods of ovulation detection in 13 cycling parous women. Histologic dating was assessed independently by two pathologists and correlated with the postovulatory duration as determined by daily transvaginal ultrasound scanning, serum LH measurements, basal body temperature (BBT), and subtraction of 14 days from the onset of menses. In addition, progesterone and estradiol (E2) were measured in daily serum samples. Dating of the endometrial biopsy was highly correlated (P less than .002) with the day of ovulation as determined by ultrasound, and was found to be within 2 days of the correct postovulatory day on evaluation of 25 of 26 (96.1%) of the interpretations. The accuracy of dating using the LH surge was 84.6% (22 of 26 interpretations), and with the BBT thermogenic shift was 76.9% (20 of 26 interpretations). However, dating of the endometrium was within 2 days of the correct day in only 17 of the 26 interpretations as determined by subtracting 14 days from the onset of the subsequent menses. The accuracy of dating was significantly better correlated (P less than .025) with days from ovulation as determined by ultrasound than as calculated from the onset of menses. There was a significant correlation between endometrial dating and the amount of progesterone (P less than .01) and E2 (P less than .01) secreted from the day of ovulation, as determined by transvaginal ultrasound, to the day of biopsy. These data confirm a strong correlation between endometrial dating and ovarian hormone secretion during the postovulatory phase.(ABSTRACT TRUNCATED AT 250 WORDS)
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This article describes the process of developing a learning needs assessment. The process includes developing a tool, collecting data, and analyzing results. Understanding the needs assessment process and the pitfalls to avoid may assist educators in implementing their own learning needs assessments.
The percentage of normal spermatozoal plasma membranes and acrosomes was evaluated by electron microscopy before and after a two-step semen washing and swim-up into culture medium containing either bovine serum albumin or hyaluronic acid. Both techniques of sperm selection increased the concentration of motile spermatozoa when compared with the fresh ejaculate (P less than .001). The percentage of sperm with ultrastructurally intact plasma membranes and acrosomes was increased after both separation procedures in comparison with the original ejaculate (P less than .01). In addition, both techniques of sperm selection significantly reduced the content of sample debris, granulocytes, and nonviable spermatozoa (P less than .01).