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Biomedical subjects

D Montagnon

Publications and source records attributed to D Montagnon.

32 records · Page 2Linked to original sources
PubMed1986

[Peroperative spinal cord echography].

Real time intraoperative ultrasonography is used since 1982 in spinal surgery. It gives numerous information, so that the neurosurgeon may adapt his technique to the obtained views. The authors describe this technique and give their experience based on 25 preliminary cases and on the literature. The normal echoanatomy of the spinal cord and the spinal canal is described as well as the echostructure of the different components observed in all cases. They give afterwards the different aspects of intramedullary tumours, extramedullary lesions (intra or extradurally), spinal traumas and syringomyelia.

Humans↗
PubMed1985

Soluble seminal fluid proteins from various animal species.

When soluble seminal proteins from different animal species are studied by cellulose acetate electrophoresis, important differences can be observed. These differences may be related to the type of coitus and the storage and migration of spermatozoa in the female genital tract. Seminal fluid can play a role in spermatozoal maturation by affecting the distribution of the surface charge of spermatozoal membranes.

Albumins↗
PubMed1984

Effects of prostatic secretion on reduced sugars in human seminal plasma.

Glucose and fructose levels were determined enzymatically in liquefied human seminal plasma (whole or collected in three fractions) and in preparations obtained by DEAE ion-exchange chromatography on whole seminal plasma. When these secretions or DEAE-extracted preparations were overloaded with fructose, the level of glucose increased. This increase is greater in fraction I of split ejaculates than in fractions II and III, and is greater in solutions containing elements of prostatic secretion obtained chromatographically than in whole seminal plasma. Using increasing quantities of fructose in the overloading test, glucose levels reached a maximum, suggesting that the process of conversion of fructose into glucose appears to be related to an enzymatic process. Other experiments suggest that the sperm-free fraction I of split ejaculates and compounds obtained from seminal plasma by ion-exchange chromatography contain enzymes of prostatic origin that are involved in the conversion of fructose to glucose.

Carbohydrate Metabolism↗
PubMed1983

[Relation between spermatozoids and reducing sugars (glucose and fructose) in human seminal fluid].

The prostatic fraction of seminal plasma contains components that are involved in the process of transforming fructose into glucose. If a positive correlation can be found between the slope of the decrease of fructose (estimated to be between 30 minutes and 5 hours) and the sperm count, with at the same time the maintenance of a relatively constant glucose level, then we have been able to demonstrate that glucose is more efficient than fructose to keep spermatozoa mobile. The enzyme process involved in transforming fructose into glucose may thus act to maintain definite glyspermia with a maximum value of 1.8 g/l. Aspermia in some circumstances then can be related to the absence or poor function of the transformation process and thus with the absence or decrease in prostatic secretion.

Fructose↗
PubMed1981

Pathological seminal plasma proteins by cellulose acetate electrophoresis.

Pathological seminal liquids were studied by using cellulose acetate electrophoresis as well as biochemical doses of proteins, acid phosphatases, reducing sugars, and zinc. It was also possible to show the frequent total absences of vesicular secretion, less frequent than prostatic secretion and less than the two at the same time. It was also shown that reductions were more frequent in prostatic secretion than in vesicular secretion. This method may also be a valuable assistance in the diagnosis of certain urogenital infections. The dissociation between prostatic secretions of acid phosphatases and zinc may lead to the diagnosis of the infection in the posterior or lateral lobes of the prostate.

Acid Phosphatase↗
PubMedDate not supplied

Studies on the origin and nature of seminal proteins by cellulose acetate electrophoresis.

Electrophoresis of semen on cellulose acetate strips allowed the localization of 19 protein bands. Twelve of these were analyzed for their composition and ten for their tissue of origin. Four bands were composed of prostatic products, three contained secretions form the seminal vesicles, and various serum products composed six others. Assays were also performed for acid phosphatase activity, glycoproteins and proteins bound to zinc. Proteins in semen were found to be broken down with time during incubation at 37C and therefore only fresh specimens or material which is frozen immediately after liquefaction may be used with these techniques. Our experiments clearly show that electrophoresis of semen may be a useful technique in clinical andrology: in particular in the case of excretory azoospermia and infections of the genital tract.

Acid Phosphatase↗
PubMedDate not supplied

Fructose, proteins and coagulation in human seminal plasma.

Fructose is produced and secreted by the seminal vesicles and it is believed to form tight bonds with certain proteins that are also of vesicular origin. Under normal conditions of liquefaction, fructose is totally dialyzable; however, blocking liquefaction by exposing semen to acidic conditions renders this sugar non dialyzable. Fructose therefore appears to play a role in the coagulation of semen. The formation of fructose-protein complexes may resolve physiological problems concerning osmotic pressure in the seminal vesicles: if all the fructose were present in free state, the osmotic pressure would be very high and incompatible with cell survival

Dialysis↗
PubMedDate not supplied

Semen parameters and capacitation.

A relationship exists between several semen parameters and the ability of spermatozoa to capacitate. The increase of oligoteratozoospermia, which could be related to testicular dysfunction, is correlated with the decrease of the percentage of spermatozoa able to "capacitate". The increase of loss of motility with time, which could be related to abnormalities of the seminal fluid, is also correlated with a decreased number of spermatozoa able to "capacitate".

Adult↗
PubMedDate not supplied

Comparative electrophoretic study between rabbit and human seminal fluid.

Cellulose acetate electrophoresis of rabbit seminal fluid give, after amidoschwartz staining, 19 protein bands, all migration towards the anode. Nine of them are present in at least 80% of the samples. With the acid of specific detection techniques, it was possible to demonstrate th presence of zinc in four bands, acid phosphatase activity in two and glycoprotein in three of the bands. Serum compounds seem to be present in small quantities. Study of fluids squeezed out from prostate, paraprostate, seminal vesicle and epididymal tail show few specific bands in these secretions: four in the case of prostate, three for paraprostate and one for seminal vesicle. Comparisons between human and rabbit seminal fluid electrophoresis show no relationship between these two types of secretion.

Acid Phosphatase↗
PubMedDate not supplied

Comparative study of semen in diabetic and healthy men.

This study examines the consequences of diabetes on male reproductive function. We have studied a group of 18 diabetics (15 type I, 3 type II) age 24 to 40 years (mean 34 +/- 5.3) with duration of diabetes from 2 to 30 years (mean 11.8 +/- 8.3). This group was compared to 20 semen donors. In diabetics higher sperm (p = 0.0063) and round cell (p = 0.031) concentrations/ml as well as a higher total spermatozoa count (p = 0.023) were observed. The percentage of abnormal sperms were also higher. None of the couples had consulted for sterility and they had a mean of 2 children. In this group of patients, diabetes was not a cause of subfertility. Specific spermiological alteration in diabetics might reflect a testicular damage secondary to their diabetes or disease.

Adult↗