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Biomedical subjects

D Mihalache

Publications and source records attributed to D Mihalache.

66 records · Page 4Linked to original sources
PubMedDate not supplied

[Immunotherapy with autovaccines in urinary tract infections caused by gram-negative bacteria].

The efficiency of immunotherapy with autovaccines was determined in 162 patients with urinary infections produced by Gramnegative bacteria. In each case quantitative urocultures were performed, with examination of the urinary sediment, and blood samples taken before and after treatment with autovaccines in order to determine the agglutination and hemagglutination titer with autovaccines in order to determine the agglutination and hemagglutination titer with regard to the incriminated germs. The latter were identified by their enzymatic and serologic characters and their sensitivity to chemotherapeutical agents. Autovaccines were prepared (2, 3 or even 4 series), repeating the tests after administration of the vaccines. Following immunotherapy, 87 patients (53.7%) presented repeated negative urocultures, 11 presented alternate positive and negative cultures, and 64 remained positive in spite of the treatment applied. There was an increase in hemagglutination titers in the patients with negative urocultures following autovaccination. To conclude it is assumed that the efficiency of immunotherapy with autovaccines depends upon their administration at the onset of infection after the patient has benefitted by the chemotherapeutical treatment. In the cases in which germs belonging to different taxonomic groups are repeatedly isolated in urocultures, it is recommended to prepare a polyvalent autovaccine to be applied in 2-3 series.

Bacterial Infections↗
PubMedDate not supplied

Determination of serum myoglobin by the reverse passive hemagglutination assay (RPHA) and radioimmunoassay (RIA).

Serum myoglobin (Mb) is an important biological marker in the early diagnosis of the acute myocardial infarction (AMI). In a previous paper we showed that RPHA, which consists in the agglutination of anti-Mb antibody--coated erythrocytes by solutions containing Mb, is a simple, rapid and highly sensitive assay (approx. 1 ng/ml). 42 sera from AMI patients and 20 sera from healthy subjects were investigated by RPHA. The geometric mean (x/divided by SD) of serum Mb titers in AMI patients was 362 x/divided by 3.94, in the range of 64-8192 (the reciprocal dilutions) and in normal sera was 19 x/divided by 1.36, in the range of 16-32. The difference between the mean values was statistically significant at p < 0.001. Subsequently, serum Mb concentration was determined by RPHA and RIA in 51 AMI sera prelevated at various stages of the disease. A high degree of correlation was found between the two methods. Log Y (RPHA titers) = -0.9174 + 1.5879 log X (RIA); ESE: +/- 0.1947; r = 0.9601. In four AMI patients successive determinations were made at every 6 hours: serum Mb curves determined by RPHA and RIA had a parallel evolution and this was an additional argument for the validation of RPHA. Being quite simple and easy of execution RPHA is the most suitable method by which an early AMI diagnosis can be made.

Blood Donors↗
PubMedDate not supplied

[The streptococcal and staphylococcal toxic shock syndrome. Comments on 4 cases].

Between 1989 and 1995 in the Hospital of Infectious Diseases from Iaşi have been diagnoses 4 cases of streptococcal/staphylococcal toxic syndrome. Three patients have been grown up and one child. Two of them were immunocompromised hosts (cirrhosis, lung tuberculosis, alcoholism). The gate of entry was cutaneous in 3 cases and probably mucous in the 4th case. The clinical symptoms were fever, generalized erythematous rush followed by desquamation, low arterial pressure with oliguria, tachycardia, jaundice moderate, elevated ALAT. The bacteriological diagnosis was confirmed by isolating the pathogen agent from the erysipelatous placard and from blood culture. The evolution of the illness was nefavourable in 2 cases. The cause of the death was the MSOF (multiple systemic organic failure).

Adolescent↗
PubMedDate not supplied

[Postoperative and posttraumatic meningitis. Comments on 87 cases].

Iatrogenic and traumatic cerebromeningitis infections are increasing in frequency in the last two decades. Retrospective analysis of 87 patients iatrogenic and traumatic bacterial meningitis were admitted in the Clinic of Infectious Diseases from Iaşi between 1, 01, 1990-31, 12, 1994. Head trauma, lumbar punctures and iatrogenic meningitis infections were the causes cerebromeningitis infections. The diagnosis is usually difficult because of the poor specificity of the clinical signs. Predominant pathogens were gram-negative bacteria and Staphylococcus in iatrogenic meningitis and gram-positive bacteria (S. pneumoniae) in traumatic meningitis. The treatment was based on the use penicillin G + chloramphenicol, 3rd generation cephalosporins and sometimes 2nd generation quinolones. Ten of the 87 patients with iatrogenic and traumatic cerebromeningitis infections died (10.3%).

Anti-Bacterial Agents↗