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Biomedical subjects

D McKenzie

Publications and source records attributed to D McKenzie.

At least 73 records · Page 4Linked to original sources
PubMed1984

Serum-IgE levels in rural Namibian infants.

Serum IgE levels were examined in 237 infants ages 2 wk to 12 mo in a remote rural area of Namibia. There was a wide range of values (0.5 to 884 IU/ml). The highest value in the first month of life was 295 IU/ml. Median values for the age groups 2 wk to 3 mo, 3 to 6 mo, and 6 to 12 mo were higher than those reported from Western countries. Values in general increased with age, but only 4% of the variation is explicable on this basis. There were differences between median values for male and female infants, but they did not reach statistical significance at the 5% level. Levels between Baster and Nama infants, the main ethnic groups in the area, did not differ. The median IgE levels in breast-fed infants, although high, tended to be lower than those in weaned infants. The differences were not statistically significant (p greater than 0.05). Investigations did not suggest that parasitic infestations or atopy were of significant importance. It was not possible to identify the factor(s) responsible for the high IgE levels. They must have been operative from very early life. However, the basic immunologic mechanisms involved are presumably genetically determined and similar to those responsible for the corresponding high IgG, IgA, and IgM levels reported in infants from developing communities.

Breast Feeding↗
PubMed1983

Sequence homologies in the protamine gene family of rainbow trout.

We have sequenced five different rainbow trout protamine genes plus their flanking regions. The genes are not clustered and do not contain intervening sequences. There is an extremely high degree of sequence conservation in the coding and 3' untranslated regions of the gene. Downstream sequences exhibit little homology though conserved regions are found 250 base pairs 3' to the gene. There are four regions upstream of the gene that are highly conserved in the six clones, including the canonical Goldberg - Hogness box which is 45 base pairs 5' to the coding region. A second homologous region is found 90 bases upstream. Although in the same approximate location as the CAAT box found upstream of other genes, it does not contain the canonical CAAT sequence. Further upstream of the protamine genes at -115 there is an A-T rich sequence while a 25 base pair conserved sequence is located 150 bases upstream. In addition we report the presence of a potential Z-DNA region of predominantly A-C repeats approximately one kilobase downstream of one of the genes.

Amino Acid Sequence↗
PubMed1983

Azthreonam: in vitro activity against urinary pathogens.

The in vitro activity of azthreonam, a novel monocyclic beta-lactam antibiotic, was investigated for Gram negative urinary pathogens by determining the susceptibilities of 105 consecutive isolates from specimens submitted to the Auckland Hospital microbiology laboratory. All were sensitive or of intermediate sensitivity by disc diffusion testing. Minimum inhibitory concentrations (MICs) were 0.25 mg/l for 97 per cent of Enterobacteriaceae and ranged from 2-16 mg/l for Pseudomonas aeruginosa. Of antibiotics tested routinely in this laboratory only tobramycin showed comparable potency.

Anti-Bacterial Agents↗
PubMed1983

Pressurized aerosol inhaler technique: how important are inhalation from residual volume, inspiratory flow rate and the time interval between puffs?

Forty-three patients with obstructive airways disease took part in three studies designed to determine the importance of three modes of inhalation of a beta 2-agonist on bronchodilator response. Under the conditions of the test, lung volume at the time of aerosol release and the time interval between the 2 puffs had no significant effect upon bronchodilator response, but a fast inspiratory flow rate was found to be associated with a significant reduction in response.

Adolescent↗
PubMed1980

Jan Hendrik Louw.

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Bibliographies as Topic↗
PubMed1976

An artefact in lactate dehydrogenase isoenzyme patterns, assayed by fluorescence, occurring in the serum of patients with end-stage renal disease requiring maintenance haemodialysis.

In patients with end-stage renal disease requiring maintenance haemodialysis we have observed that serum albumin becomes fluorescent (excitation 356 nm, emission above 405 nm). This fluorescence is detected in the fluorescent assay of lactate dehydrogenase (LDH) isoenzymes on thin-layer agarose creating the impression of an additional LDH isoenzyme anodal to LDH1. This artefact disappears, or is much reduced, following successful donor kidney transplantation. It is not seen in any other pathological condition. This phenomenon can readily be detected by means of "blank" assays on the agarose plates after specimen application and after electrophoresis of the specimen without the substrate overlay.

Acute Kidney Injury↗
PubMed1976

How accurate are lactate dehydrogenase isoenzyme estimations by the thin-layer agarose fluorescent technique?

We offer an assessment of the accuracy of the thin-layer agarose fluorescent technique of Elevitch et al. [Am. J. Clin. Pathol. 46, 692 (1966)]. We used semi-purified human lactate dehydrogenase isoenzymes 1 and 5. Both the lactate lead to pyruvate and pyruvate lead to lactate assays [Clin. Chem. 20, 1462 (1974)] appear to give, within the errors of the techniques used, a substantially unbiased estimate of both LD-1 and LD-5, although this must remain a provisional conclusion until a definitive method of assay for the total and isoenzymic LD activities is created. Introducing a buffer into the substrate mixture (lactate lead to pyruvate assay) had no effect on these findings except at extremes of pH, when marked inaccuracies occurred.

Buffers↗