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Biomedical subjects

D Marcus

Publications and source records attributed to D Marcus.

At least 55 records · Page 3Linked to original sources
PubMed1983

Human lymphoblastoid interferon for clinical trials: large scale purification and safety tests.

Human lymphoblastoid cell lines are one of the best sources for the production of large amounts of human interferon for clinical trials. The potential risk of using transformed cell lines for production of a substance for human use can be overcome by an extensive purification of the interferon to a high degree, and by carefully testing the final product for DNA content and toxic materials. In previous reports we described a method for large scale production of interferon by a human lymphoblastoid cell line (Namalva). In this report a system for large scale purification of Namalva interferon and safety tests is presented. The procedure consists of three main steps: 1. Concentration of crude interferon by ultrafiltration. II. Gel filtration on Sephadex G-75 or Ultrogel AcA 54 columns. III. Affinity chromatography on anti-interferon antibody column. All steps were adapted for the processing of batches of hundreds of litres and can be easily further scaled-up. The final highly purified product undergoes a series of quality control tests such as purity, sterility, toxicity, pyrogenicity and DNA content. After passing all tests the IFN is released for clinical trials.

Cell Line↗
PubMed1981

Posterior aortic wall motion and left atrial volume changes.

The motion characteristic of the posterior aortic wall has been used in precordial M-mode echocardiography as a parameter of cardiac function. However, the determinants of this characteristic motion pattern of the aorta are still unknown. In this study the posterior aortic wall motion was studied angiographically as it is related to left atrial volume changes. 21 patients with various heart diseases served as the study group. 18 patients were in sinus rhythm, 3 patients in atrial fibrillation. We found an excellent agreement between left atrial volume changes and the movement of the posterior aortic wall. The correlation coefficient between both parameters ranged from .73 to .95 irrespective of the underlying heart disease and heart rhythm. From this study one can conclude that left atrial volume changes are reflected by the motion of the posterior aortic wall.

Adult↗
PubMed1980

Synthesis of human plasminogen by the liver.

Genetic types of plasminogen were determined from a donor and a recipient before and after hepatic homotransplantation. Examination of the plasminogen types demonstrated that the liver is the principal site of synthesis of human plasminogen.

Female↗
PubMed1980

Genetic polymorphism of human plasminogen.

Using isoelectric focusing (IEF) in polyacrylamide gel of neuraminidase-treated serum or plasma samples and immunofixation or caseinolytic overlay after urokinase activation of gels, a common genetic polymorphism in human plasminogen has been delineated. Two alleles PLGN*A and PLGN*B, were observed with gene frequencies in whites of .69 and .30; in Orientals of .96 and .03; and in blacks of .80 and .18. Several rare alleles were also found. The distribution of phenotypes fits the Hardy-Weinberg equilibrium. Inheritance is autosomal codominant and fits the expectations of Mendelian inheritance. There is fetal synthesis, but no transplacental passage of plasminogen in either direction.

Adult↗
PubMed1978

Staphylococcus aureus bacteremia: relationship between formation of antibodies to teichoic acid and development of metastatic abscesses.

Of 50 patients with bacteremia due to Staphylococcus aureus but without clinical evidence of endocarditis, 24 developed antibodies to the cell wall teichoic acid of S. aureus that were demonstrable by counterimmunoelectrophoresis. However, only 16 of the 24 patients developed titers of antibodies high enough for detection by passive gel diffusion. Eleven of the 16 patients developed evidence of complications due to metastatic infection. In contrast, of the 34 patients who were antibody-negative by gel diffusion, only one patient developed evidence of metastatic seeding. Thus, the development of antibodies to teichoic acid at a level detectable by the gel diffusion technique is regularly associated with complicated infections due to S. aureus that require more prolonged therapy, whereas bacteremic patients not developing such an antibody response rarely develop complications and may be treated with a two-week course of therapy.

Abscess↗
PubMed1977

Heroin-associated rhabdomyolysis with cardiac involvement.

A heroin addict had rhabdomyolysis with cardiac involvement. The patient was admitted with edema of the right leg and oliguria. Admission diagnoses were right illofemoral thrombophlebitis, acute renal failure, and heroin addiction. Urinalysis was strongly positive for "blood" in the absence of hemolysis or marked hematocyturia, and a diagnosis of rhabdomyolysis was made. Peritoneal dialysis succeeded in lowering blood urea nitrogen and serum potassium levels, but the patient died on the fourth hospital day. Postmortem examination disclosed focal myocardial myolysis, diffuse rhabdomyolysis of the right soleus muscle, and acute renal tubular necrosis. Direct toxicity or hypersensitivity to heroin or an adulterant is considered in the pathogenesis of myolysis.

Adult↗