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Biomedical subjects

D Mackay

Publications and source records attributed to D Mackay.

At least 109 records · Page 6Linked to original sources
PubMed1971

Diffusion of labelled substances through isolated rat diaphragm.

1. The rate of diffusion of [(14)C] sucrose through rat diaphragm muscle is dependent on the period of preliminary soaking of the muscle in Krebs solution at 36 degrees C. The ratio of the length of the diffusion path through the diaphragm to its geometrical thickness appears to fall from 4.39+/-0.38 (S.E. of the mean) to 2.23+/-0.18 after about 6 h of soaking.2. The area of the diaphragms available for diffusion is apparently constant at 0.3-0.4 of their total area.3. The measured thickness of the diaphragms increases by about 20% on soaking.4. Entry of [(131)I] iodide into the muscle fibres is detected as a fall in both steady state and non-steady state rates of diffusion through the diaphragm.5. Entry of [(14)C-methyl] decamethonium into the muscle cells is not evident from such diffusion studies in normal Krebs solution.

Animals↗
PubMed1967

The use of the logarithmic transformation in the calculation of the transport parameters of a system that obeys Michaelis-Menten kinetics.

1. A logarithmic method is described for the calculation of the transport parameters, K(m) and V(max.)' of a biological system obeying Michaelis-Menten kinetics. 2. This logarithmic method leads to a way of estimating the transport parameters that has not apparently been used previously. It allows the separation of variance due to V(max.) from other variance, and so reduces the fiducial limits that can be placed on an estimation of K(m). 3. The results of studies on the transport of l-histidine and l-monoiodohistidine by rat intestinal sacs in vitro have been used to illustrate the application of the new method. Estimates of the transport parameters have also been made by two alternative procedures. The relative merits of the three methods are discussed.

Animals↗