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Biomedical subjects

D M Ranly

Publications and source records attributed to D M Ranly.

At least 37 records · Page 2Linked to original sources
PubMed1985

The effect of alternatives to formocresol on antigenicity of proteins.

As part of an overall evaluation of possible substitutes for the pulpotomy agent formocresol, this study was initiated to compare the antigenicity of the reaction products of protein with formaldehyde, glutaraldehyde, or dimethylsuberimidate (DMS). Rabbits were injected with rabbit serum albumin (RSA) which had been treated with one of the following solutions: phosphate-buffered saline, 2% glutaraldehyde, 4% formaldehyde, or 2% DMS. The antisera from the rabbits were analyzed for elicited antibodies by the enzyme-linked immunosorbent assay (ELISA) and in a horseradish peroxidase (HRP) assay using a spot technique on nitrocellulose paper. These assays demonstrated that DMS-treated RSA was the most antigenic of the reaction products tested. The least provocative was the glutaraldehyde-treated RSA; the reaction product of formaldehyde was intermediate. Our findings suggest that if non-immunogenicity of a pulpotomy agent is a desirable property, then DMS does not meet the criteria of an alternative pulp fixative. In contrast, the relatively low antigenicity of glutaraldehyde reinforces other favorable findings which support its use clinically.

Aldehydes↗
PubMed1983

A biochemical study of two bifunctional reagents as alternatives to formocresol.

Two bifunctional reagents, glutaraldehyde and dimethylsuberimidate, were compared to formaldehyde with respect to fixation and modification of protein. Glutaraldehyde proved the superior fixative based on cross-linking and enzyme degradation assays. An immunoprecipitation assay suggests that the reaction products of glutaraldehyde may have altered antigenicity.

Aldehydes↗
PubMed1982

The effect of formocresol and glutaraldehyde on certain enzymes in bovine dental pulp.

The effect of a 2-hour formocresol and glutaraldehyde treatment on two enzymes of bovine pulp was measured. Lactic dehydrogenase, a respiratory enzyme, was sharply affected by 0.5 percent and one percent glutaraldehyde and a 1:5 dilution of formocresol, exhibiting 7-, 71-, and 40-fold decreases in activity, respectively. Alkaline phosphatase was much less responsive to these same agents, giving only 4.5-, 17-, and 2.5-fold reductions after treatment, respectively. These findings support histochemical studies which have suggested the sensitivity of respiratory enzymes of the pulp to fixative medicaments.

Aldehydes↗
PubMed1978

Biochemical effects of formocresol on bovine pulp tissue.

Calf pulp was treated with full-strength formocresol, diluted formocresol, or saline for 4 hours. After washing and homogenization, the water extractable supernates were analyzed for total amino acid, carbohydrate, and hydroxyproline content. Additional samples were tested against trypsin, pepsin, collagenase, and hyaluronidase. Other tissue samples treated with 1/5, 1/10, and 1/25 dilutions of formocresol were subjected to trypsin and collagenase. The control tissue gave 50 per cent more extractable material, which contained over 300 per cent more total amino acids and hydroxyproline but only slightly more carbohydrate than the treated tissue. Formocresol treatment produced an 80 to 90 per cent reduction in reactivity to trypsin, pepsin, and collagenase but little change from hyaluronidase action. The increase in reactivity of the tissue to enzyme hydrolysis paralleled the increase in dilution of formocresol. These results indicate a profound effect on the protein fraction of pulp exposed to full-strength formocresol.

Animals↗