Search PubMed⌕ Search

Biomedical subjects

D M Morgan

Publications and source records attributed to D M Morgan.

At least 73 records · Page 4Linked to original sources
PubMed1982

Serum polyamine-oxidase activity in spontaneous abortion.

Serum polyamine oxidase activity was determined in 38 women admitted to the Maternity Department of Szombathely, Hungary with clinical signs of threatened abortion between 11 and 22 weeks gestation and who aborted spontaneously with 48 h of admission. Serum polyamine oxidase activity was found to be significantly lower in these women compared with that in women having normal pregnancies of the same gestational age. The higher polyamine oxidase levels which were found in five women who aborted during oxytocin infusion probably resulted from increased contractions expelling enzyme-rich blood from the intervillous space into the maternal circulation. Present evidence is insufficient to explain the full clinical significance of these findings.

Abortion, Spontaneous↗
PubMed1982

The serological response of chickens to oil emulsion vaccines containing the V4 strain of Newcastle disease virus.

Experiments were conducted with vaccines containing the V4 strain of Newcastle disease virus (NDV). Both living aqueous vaccines and vaccines consisting of virus incorporated oil emulsion were used. The calculated dose of virus contained in the oil emulsion vaccine was 10(8.7) 50% embryo infectious doses (EID50) per bird dose. Haemagglutinin inhibition (HI) antibody levels of 8 are presumed protective. One-day-old chicks with low levels of maternal antibody were vaccinated intraocularly with 10(6.3)EID50 ol live vaccine, and concurrently with oil emulsion vaccine. Presumed protective levels of antibody were present at two weeks post vaccination and were maintained for at least seven weeks longer. When adult birds 15 weeks old with no previous exposure to NDV were vaccinated intraocularly with 10(6.7)EID50 per bird, protective levels of antibody were produced within a week. Unvaccinated birds put in contact with the vaccinated birds produced similar antibody levels within 14 days. Revaccination with oil emulsion vaccine after antibody levels had fallen resulted in a rapid response with high levels of antibody. When antibody-free adult commercial birds with an unknown history of exposure to NDV were vaccinated intramuscularly with oil emulsion vaccine, high antibody levels were produced for at least 21 weeks. Concurrent intraocular inoculation with 10(7.0)EID50 live virus did not enhance the response. Natural infection of unvaccinated birds occurred during the experiment. This was detected by the presence of HI antibody levels of short duration. When antibody-free commercial birds were inoculated intramuscularly with oil emulsion vaccine containing 10(6.0), 10(7.0), or 10(8.0)EID50 per bird dose, 100% of birds inoculated with the highest dose produced presumed protective levels of antibody within two weeks, as compared with a 5-week delay when using the 10(7.0)EID50 per bird dose.

Animals↗
PubMed1981

Analysis of a restriction endonuclease map for bovine papillomavirus type 2 DNA.

A physical map was constructed for bovine papillomavirus type 2 DNA by the use of restriction endonucleases. A comparison between the genomes of bovine papillomavirus types 1 and 2 in regard to location and number of cleavage sites of seven enzymes is also presented. This comparison revealed similarities between the two genomes.

Bovine papillomavirus 1↗
PubMed1980

Polyamine-polyamine oxidase interaction: part of maternal protective mechanism against fetal rejection.

Human retroplacental blood serum significantly (p less than 0.01) suppresses the in-vitro uptake of 3H-thymidine--that is, synthesis of deoxyribonucleic acid--by spontaneously growing human lymphocytes in the presence of exogenous spermine, but only in concentrations with a higher polyamine oxidase activity than that found in maternal peripheral blood serum during pregnancy. These findings together with observations that the placenta is rich in spermine and that interaction of polyamine oxidase and substrate arrests cell proliferation suggest that such interaction might represent a localised immunoregulatory mechanism in the placental bed, which might contribute to the protection of the fetoplacental unit from possible maternal immune rejection.

Cell Division↗
PubMed1980

Polyamine oxidase activity in amniotic fluid and fetal membranes.

Polyamine oxidase activity, already shown to be present in maternal serum, was measured in amniotic fluid between 15 and 40 weeks of gestation in 38 samples. The enzyme activity increased as pregnancy progressed, the levels being lower than those found in the maternal serum. Homogenates of chorion, amnion, separately and together, also showed the presence of enzyme activity, the levels being lower than those previously found in the decidua, but substantially higher than those found in the placenta. Ii is suggested that the enzyme found in amniotic fluid might be the consequence of a diffusion from the decidua through the membranes.

Amniotic Fluid↗
PubMed1979

The distribution of polyamine oxidase activity in the fetomaternal compartments.

Polyamine oxidase activity was measured in different compartments of the feto-maternal unit by a radiochemical method. The activity in the retroplacental serum (mainly of intervillous origin) was 20 to 30 times higher than in maternal uterine or peripheral venous blood sera. No activity was found in the fetal cord blood sera. The enzyme level in the maternal peripheral sera fell to undetectable levels within 72 hours post partum. Preliminary data indicate that the enzyme might be produced by the decidua rather than by the placenta. It is suggested that the enzyme may constitute an important part of a supposed local immunological barrier formed at the placental bed to help to protect the fetal allograft from maternal immune rejection through a local suppressive effect on maternal cellular immunity.

Cesarean Section↗
PubMed1979

Polyamine oxidase activity in human pregnancy serum.

The existence of a spermine oxidizing enzyme was established in human pregnancy serum by a radiochemical method, and a cross-sectional study of its quantitative profile between 10 and 40 weeks gestation was undertaken. Though there was a wide scatter of values, the rise in the average activity was continuous and highly significant. The flavoprotein inhibitor, quinacrin, inhibited the enzyme activity, while the pyridoxal phosphate inhibitor, isoniazid, had no significant inhibitory effect suggesting that the enzyme is of tissue origin. The possible role of polyamines and their oxidation in physiological immunoregulation is discussed.

Cross-Sectional Studies↗