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Biomedical subjects

D M Miller

Publications and source records attributed to D M Miller.

At least 235 records · Page 13Linked to original sources

Fatty acids in female Macracanthorhynchus hirudinaceus (Acanthocephala).

Neutral lipids and phospholipids in the body wall, lemnisci and pseudocoel and neutral lipids of fluid found in the "tube" system of the lemnisci and the lacunar system of the body wall of Macracanthorhynchus hirudinaceus (Acanthocephala) were determined by the technique of thin layer chromatography and gas chromatography (GC). Sixteen different fatty acids from nonpolar lipids were identified as follows: 8:0, 10:0, 11:0, 12:0, 13:0, 14:0, 14:1, 16:0, 18:0, 18:1, 18:2 and/or 20:0, 18:3 and/or 20:1, 20:3, 22:1, 24:1 and 22:6. In addition, there were three unidentified GC peaks corresponding to chain lengths greater than 20 carbons. Sixteen different fatty acids from phospholipids were identified in each of the three fractions analyzed. They were as follows: 10:0, 11:0, 12:0, 13:0, 14:0, 14:1, 16:0, 16:1, 18:0, 18:1, 18:2 and/or 20:0, 20:2, 20:3, 22:1, 24:1 and 22:6. Four unidentified peaks were also observed. There was a significant difference in the percentage of lipid as well as the concentration of particular fatty acids when each fraction was compared. There was also an abundant supply of sterols and glycerides in each fraction.

Acanthocephala↗

Distal displacement of the maxilla and the upper first molar.

Data from a sample of 198 Class II cases treated with various appliances which deliver distally directed forces to the maxilla were examined to determine the frequency of absolute distal displacement of the upper first molar and of the maxilla. Analysis revealed that such distal displacement is possible and that it is, in fact, a frequent finding following treatment. Long-range stability of distal displacement was not assessed.

Cephalometry↗

Electrophysiology of acanthocephalan body wall muscles.

Body wall muscles of an acanthocephalan Macracanthorhynchus hirudinaceus were studied by means of scanning and light microscopy and intracellular recording of potentials. Three types of spontaneous potential changes were found: larger (L) potentials which usually exhibited overshoot and were as large as 65 mV; smaller symmetric (A) potentials approximately 15 mV in amplitude; and even smaller asymmetric (S) potentials which sometimes reached 10 mV. The potentials recorded depended upon the position of the electrode in the anterior-posterior, as well as the medial-lateral, axis. Tetrodotoxin eliminated L but not S potentials. Ouabain lengthened the time for depolarization of L potentials and depolarized the membrane potentials. It is suggested that the rete system activates the body wall muscles in Acanthocephala.

Acanthocephala↗

The kinetics of the active and de-energized transport of O-methyl glucose in Ustilago maydis.

The kinetics of the uptake and efflux of 3-O-methyl-glucose in sporidia of Ustilago maydis were measured, both in active cells and in cells whose metabolic activity had been inhibited by azide and iodoacetate. The de-energized transport system proved to be carrier mediated with apparent affinity constants 13 +/- 2 mM outside (Ko) and 18 +/- 2 mM inside (K1). The apparent maximum rate constants for the same system were 0.66 +/- 0.05 mmol/1 cell water per min for uptake (V+) and 0.53 +/- 0.04 mmol/l cell water per min for efflux (V-). For the active system K0 = 0.08 +/- 0.01, K1 greater than 40, V+ = 9.7 +/- 0.5 and V- = 1.1 +/- 0.9 (in equivalent units). These results are discussed in the context of the carrier mechanism as proposed by Regen and Morgan (Regen, D.M. and Morgan, H.E. (1964) Biochim. Biophys. Acta 79, 151--166). The antifungal compound carboxin had no effect on de-energized transport but was shown to decrease both K0 And V+ in the active system. Phloretin and phlorizin were also found to be without effect on de-energized cells but the former enhanced while the latter inhibited active uptake.

Antimycin A↗

Anatomy of the genital ganglion of the male acanthocephalan, Moniliformis moniliformis.

Male Moniliformis moniliformis possess paired genital ganglia each measuring approximately 280 micron long by 80 micron wide by 25 micron thick. They are located on either side of Saefftigen's pouch on the ventral surface of the ejaculatory duct where it joins the bursal cap. The cellular organization consists of externally located soma and a poorly developed internal neuropile. Most of the 19 cells in each ganglion exit via axons from the anterior and posterior extremities. A single dorsal commissure connects the 2 ganglia. No ventral commissure was observed. Many cells contained large nuclei with perinuclear rings but no cell had 2 nuclei.

Acanthocephala↗

A new ganglion in male Moniliformis moniliformis (Acanthocephala).

A new ganglion identified as the bursal ganglion is described from male Moniliformis moniliformis. This ganglion is located adjacent to the pseudocoel and longitudinal muscle fibers and medial to the dorsal lacunar canal about 1 mm from the posterior end of males with non-everted bursa. The ganglion consists of four large club-shaped cells with single nuclei and bipolar neurons. The ganglion cells are paired with one neuron from each cell innervating the opposite side of the worm.

Acanthocephala↗

Effects of music-listening contingencies on arithmetic performance and music preference of EMR children.

A five-phase experiment was designed to investigate (a) whether contingent music-listening would act as a reinforcer to increase arithmetic performance of EMR children and (b) whether this contingent reinforcement would affect preference for that reinforcer. During the three baseline (no reinforcement) phases, subjects returned to their classrooms at the end of the arithmetic work period. During the two treatment phases, experimental subjects meeting criterion were permitted to remain and listen to either rock (high preference) or music classics (low preference). Statistical analyses revealed significant differences between the no-reinforcement and contingent-reinforcement conditions and between the control and four experiment groups during the contingent reinforcement phases. No differential effect between the more or less preferred contingencies was found. Analyses of the music-selection data indicated that the contingencies did not influence music preference.

Adolescent↗

Ion metabolism in a halophilic blue-green alga, Aphanothece halophytica.

The intracellular ion content of the halophilic blue-green alga, Aphanthece halophytica was studied as a function of age, external sodium and external potassium concentration. Intracellular Na+ was found to be about 0.38 millimoles/g dry mass. Intracellular K+ concentrations were as high as 1 M and varied directly with external salinity. Intracellular Ca++ and Mg++ were in the range previously reported for fresh water blue-green algae despite their extremely high extracellular concentrations. Average cell size is consistent at room temperature with two exceptions. When the outside K+ is lower than 6.5 mM the cells tend to be smaller with less intracellular K+ and high Ca++. In stationary phase cultrues the cells are larger with high intracellular Mg++ and low K+.

Calcium↗