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Biomedical subjects

D M Hood

Publications and source records attributed to D M Hood.

26 records · Page 2Linked to original sources
PubMed1989

Effect of weight loading on the coronary band interstitial fluid pressure in horses.

Interstitial fluid pressures, as a possible function of limb load, were measured at 2 sites within the digital coronary dermis of both cranial digits in 10 standing horses. Fluid pressure changes and digital load measurements were simultaneously detected and recorded by use of, respectively, modified wick-in-needle and force plate transducers coupled to a microcomputer. Mean pressures, recorded at limb loads between 50 and 80 kg, were 2.29 +/- 3.17 mm of Hg at the toe and 2.49 +/- 5.91 mm of Hg at the heel. Mean pressures, recorded between 150 and 180 kg, were 5.01 +/- 5.23 mm of Hg at the toe and 1.28 +/- 7.69 mm of Hg at the heel. These data indicate that, in the static limb, no statistically significant change in interstitial fluid pressure occurs at loads up to 180 kg.

Animals↗
PubMed1988

Epidermal growth factor-mediated effects on equine vascular smooth muscle cells.

Epidermal growth factor (EGF) receptor binding kinetics and EGF-mediated stimulation of DNA synthesis and cellular proliferation were studied in cultured vascular smooth muscle cells (VSMC) from the equine thoracic aorta. Binding studies, using murine 125I-labeled EGF, indicate the presence of a single class of high-affinity binding sites (apparent KD = 2.8 X 10(-11) M), with an estimated maximal binding capacity of 5,800 sites/cell. EGF stimulated [3H]thymidine uptake in confluent quiescent monolayers in a dose-dependent fashion, half-maximal stimulation occurring at 7.5 X 10(-11) M. Likewise, EGF-mediated cellular proliferation was dose dependent (50% effective dose = 5 X 10(-11) M) under reduced serum concentrations. Equine VSMC contain specific receptors for EGF, and EGF can stimulate DNA synthesis and proliferation in these cultured cells, which suggests that EGF may participate in the proliferative changes observed in equine distal digital peripheral vascular disease.

Animals↗
PubMed1984

Measurement of plasma antithrombin III activity in healthy horses.

A fluorometric assay was used to determine plasma antithrombin III (AT III) activities in 15 healthy adult horses. Nearly all plasma samples had an initial value of greater than 100% thrombin inhibited, so a 1:1 dilution of the prepared samples was performed. Following dilution, the mean value of the animals was 59.17 +/- 7.4% thrombin inhibited. Mares had significantly greater AT III activity than did geldings (P less than 0.01). The results of this study indicate the horse has more AT III activity than did other domestic species in which AT III activity has been reported.

Animals↗
PubMed1982

Phenoxybenzamine for the treatment of severe nonresponsive diarrhea in the horse.

Phenoxybenzamine, an alpha adrenergic antagonist, was administered IV to 6 clinically normal horses, 5 horses with experimentally induced diarrhea, and 7 horses with naturally-occurring diarrhea. It was established that a total of 2 mg of phenoxybenzamine/kg of body weight given in divided doses resulted in alpha adrenergic blockage of approximately 72 hours' duration, tranquilization, and mild constipation in the normal horse. The 5 experimental cases of diarrhea were involved in a laminitis research protocol in which laminitis was induced by oral carbohydrate overload. In all 5 of those cases, the severity of the diarrhea that accompanied the carbohydrate overload was reduced. The 7 clinical cases consisted of 2 foals and 5 adults with diarrhea, some of which were nonresponsive to conventional treatment. One of the foals did not respond to the phenoxybenzamine, and the other had a questionable response, whereas the 5 adults all responded favorably to the agent. It was concluded that phenoxybenzamine has a potential place in the therapy of some cases of diarrhea that are nonresponsive to conventional therapy. The mechanism of action of phenoxybenzamine on diarrhea is not known at this time.

Animals↗
PubMed1978

Clinical pulmonary perfusion imaging in the dog.

Scintigraphic pulmonary perfusion imaging in the dog has been described for the clinically normal animal. The usefulness of this technique in the patient with pulmonary dysfunction was assessed by review of 76 consecutive clinical cases completed in a 6-month period. This review indicated that 3 basic patterns of perfusion defects are commonly seen in the dog. These were associated with (1) diffuse parenchymal metastatic disease, (2) mediastinal metastatic disease, and (3) heartworm disease. The technique has proved reliable as an aid in the staging of neoplastic disease and of some potential value in heartworm disease and is now performed on a routine basis at this institution for purposes of clinical diagnosis.

Animals↗
PubMed1977

Control of radiation in animal facilities.

Use of radioactive materials in animals for research and clinical studies is on the increase. These studies may be undertaken with little or no disruption of normal facility operations if a few facts are considered. The primary factor of consideration is the radiopharmaceutical--its pharmacologic behavior and physical characteristics. The preferred radionuclide is one with the shortest half-life compatible with the variables to be measured. The fact that an animal is a source of radiation as well as a potential source of contamination must be kept in mind. Improper use of radiopharmaceuticals is inexcusable.

Animals↗
PubMed1976

Evaluation of radioiodinated human serum albumin in the dog for assessment of hemodynamic function.

Radioiodinated human and dog serum albumins, each labeled with a different radioisotope of iodine (125I and 131I, respectively), were injected intravenously into dogs. Plasma volumes and plasma clearance half times were used for the comparative evaluation of the 2 radiopharmaceuticals. After repeated injections in a 6-week period, differences in these values could not be demonstrated. Plasma volumes obtained with both radioiodinated serum albumins compared favourably with the previously reported values in dogs.

Animals↗