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Biomedical subjects

D M Berman

Publications and source records attributed to D M Berman.

47 records · Page 3Linked to original sources
PubMed1988

Basal and amiloride-induced short-circuit current across isolated toad skin (Bufo arenarum).

We have previously demonstrated that amiloride (amil) addition to the isolated ventral pelvic (VPel) skin of Bufo arenarum toad induces negative short-circuit current values, which are equivalent to the isotopically measured net chloride transport. In the present work, we found that exposure of various regions of toad skin to amil yielded different values of short-circuit current (aSCC): negative aSCC was found in the VPel and ventral pectoral skin, while those of the dorsal one were not different from zero. The distinct values of aSCC found show a regional difference in the active chloride absorption, probably related to postural adaptations. A possible role of this adaptation would be related to chloride participation in the saline balance of the animals, or the maintenance of epithelial integrity.

Amiloride↗
PubMed1987

Reversed short-circuit current across isolated skin of the toad Bufo arenarum.

Short-circuit current (SCC) across isolated pelvic skin of the toad Bufo arenarum has been shown to be reflected by the algebraic sum of net sodium and chloride transport. After the animals had been maintained in tap water, amiloride--an apical sodium channel blocker--led to a reversal of potential difference (rPD) across this preparation, to which corresponded a reversed short-circuit current (rSCC). Both rSCC and rPD were abolished by dermal treatment of skins with the metabolic inhibitor dinitrophenol, or by omission of chloride ion from the Ringer solution bathing both sides of the skin. There was a significant positive correlation between rSCC and isotopically determined net chloride transport after amiloride. An inhibitory action of amiloride on unidirectional chloride fluxes was detected, but only early after drug addition. rSCC was absent in skins of toads exposed to 110 mmol/l NaCl in tap water during 10 days. Together, our results suggest that amiloride addition--by inhibiting active sodium movement--can in certain conditions reveal the existence of an inward active chloride transport.

Amiloride↗
PubMed1987

Comparative effects of angiotensin II on osmotic water permeability in the toad (Bufo arenarum).

The possible relationship between the renin-angiotensin system and water balance in the toad Bufo arenarum has been indirectly explored. A positive correlation was found between the hydrosmotic response of ventral pelvic toad skin to angiotensin II (A II) and some age indicators (body weight, snout-urostyle length or head width). A different hydrosmotic response for oxytocin and isoproterenol (but not for A II) was found between four cutaneous regions of toad body. We conclude that A II may not be directly involved in the regulation of water balance mediated by water absorption across the skin of Bufo arenarum toads.

Angiotensin II↗
PubMed1986

Phenothiazines increase active sodium transport across the isolated toad skin.

Fluphenazine (FPZ) and trifluoperazine (TFP) are phenothiazine derivatives commonly used as antipsychotic tranquilizers. Their mechanism of action is incompletely understood. Epidermal addition of each drug promoted biphasic short-circuit current (SCC) changes across isolated pelvic skin of Bufo arenarum toads. By means of radiotracers fluxes, SCC was found to be given by the algebraic sum of net sodium and chloride transport. A readily stimulant effect was detected a low concentrations (from 1 X 10(-6) mol/l up to 1 X 10(-4) mol/l for FPZ, from 1 X 10(-5) mol/l up to 3.2 X 10(-4) mol/l for TFP) above which inhibition prevailed. Dermal FPZ also stimulated SCC. A higher concentration and time threshold were required. Epidermal 1 X 10(-5) mol/l FPZ stimulation was partially reversible, with a diminished membrane resistance and enhancement of sodium influx, without alteration of sodium efflux or net chloride transport. It could be prevented by amiloride pretreatment, or diminished by dermal sodium removal. Variation of epidermal bulk pH from 5.8 to 8.7 demonstrated that ionized and nonionized molecules contribute to FPZ's effect. Our results suggest that SCC stimulation elicited by FPZ and TFP may be a consequence of direct or indirect modifications on apical sodium conductance.

Amiloride↗
PubMed1986

Angiotensin converting enzyme in the toad Bufo arenarum.

Angiotensin converting enzyme activity (ACEA) was determined in serum, kidney, whole skin and isolated epithelia homogenates of the South American toad Bufo arenarum. ACEA was present in the tissues and serum of the toad. The activity was higher in the kidney, as compared to that of the whole skin or isolated epithelium. Captopril, teprotide and EDTA, caused a significant decrease in the ACEA. Possible physiological roles for the presence of ACEA in the toad are discussed.

Animals↗
PubMed1986

Asbestos and health in the Third World: the case of Brazil.

Almost all of the asbestos used in Brazil is mined by an enterprise wholly owned by two European multinational companies, which also produce and market over two-thirds (by weight of asbestos) of the products made from asbestos. About 80 percent of the asbestos used in Brazil is finally consumed in the form of asbestos cement: for roof tiles and roofing panels, wall-board, and domestic and industrial water tanks. A survey of consumer literature and advertising printed by Eternit, S.A., and Brasilit, S.A., disclosed no mention of a potential danger from exposure to asbestos dust, and no recommendations for cutting down exposure to that dust. The situation at smaller, Brazilian-owned firms is reputed to be disastrous from the standpoint of workers' exposure to asbestos dust at the point of production. At a large asbestos-cement manufacturing plant owned by Eternit, however, exposure to asbestos dust (according to company records) seemed to be kept under 2.0 fibers per cc., the present standard for the United States.

Air Pollutants, Occupational↗
PubMed1985

Inhibition of stimulated osmotic water flow by fluphenazine, a calmodulin inhibitor, in the isolated toad skin.

The effects of fluphenazine, a phenothiazine calmodulin inhibitor, on the osmotic water flow (Posm) across isolated skins of Bufo arenarum toads were tested. Fluphenazine inhibited the increase in Posm induced by agents known to act through the cyclic AMP system (oxytocin, db-cAMP, theophylline, KCl and isoproterenol). The inhibitory effect was faster and more intense when the drug was present in the epidermal bath, and persisted after rinsing the preparation for 100 min. Our results indicate that phenothiazine's action may be primarily exerted at a site distal to cyclic AMP generation.

Animals↗
PubMed1978

How cheap is a life?

The oweners of capital in the United States have successfully transferred most of the costs of industrial casualties onto the working class and the public at large. This has been accomplished by the creation of the privately owened workers' compensation insurance system and the corporate-dominated safety establishment. This "compensation-safety establishment" has been able to take over most of the federal apparatus created by the Occupational Safety and Health Act of 1970. Nevertheless, workers and unions and their allies have begun to challenge the establishment's hegemony over job health and safety policy for the first time in seventy years.

Accident Prevention↗
PubMed1977

Why work kills. A brief history of occupational safety and health in the United States.

In the early 20th century, U.S. monopoly corporations responded to the movement against work accidents by setting up a business-controlled "compensation-safety establishment," which kept down compensation costs but did little to improve working conditions. The "establishment" was able to keep the issue of occupational safety and health out of public debate until the late 1960's through its control of research, education, compensation, and government appointments in the area, and by creating the public impression that the problems of occupational disease were almost nonexistent. Despite the occurrence of sporadic rank-and-file uprisings, unions have been seriously involved in health and safety only since the late 1960's, when they mobilized in an effort to pass the Occupational Safety and Health Act of 1970. The passage of the OSHA law was made possible by the help of progressive professionals, worker dissatisfaction, the new environmental consciousness, and a general climate of social unrest. Although the corporate elite, through the "compensation-safety establishment," has been able to dominate the operation of the federal institutions created by the new law, the question of occupational health and safety is now on the permanent agenda of workers, unions, and the public.

Accidents, Occupational↗