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Biomedical subjects

D Liu

Publications and source records attributed to D Liu.

At least 361 records · Page 20Linked to original sources
PubMed1999

[Changes of insulin and C-peptide in pregnancy of gestational diabetes mellitus].

OBJECTIVE: To observe the different changes of plasma insulin and c-peptide in patients with gestational diabetes mellitus (GDM), gestational impaired glucose tolerance (GIGT), normal pregnant women, and to detect the function of beta-cell in patients with gestational impaired glucose metabolism. METHODS: Test the plasma insulin, c-peptide in 48 cases of GDM(group I), 39 cases of GIGT (group II), 39 cases of normal pregnant women (group III) and 22 cases of healthy non-pregnant women (group IV) by radioimmunoassay. RESULTS: (1) In third trimester of group III, insulin, c-peptide levels were higher than those of group IV (P < 0.01). They increased from 28-34 weeks to 38-40 weeks, peaked during delivery. Insulin levels of group I and group II were higher than those of group III (P < 0.01, P < 0.05), but tended to decrease by the end of the third trimester and peaked during delivery. During delivery, there was no significant differences among the three groups. At 28-40 weeks, c-peptide of group I was significantly higher than those in groups II and III (P < 0.01) and was lower than that in group III during delivery (P < 0.05). Insulin and c-peptide of these 3 groups dropped gradually within 2 months after delivery but in group I and II they were significantly higher than those of group III (P < 0.01). CONCLUSION: In third trimester insulin and c-peptide of group I-III increased till delivery, the reason maybe associated with insulin resistance. GDM had the same peaking secretion during delivery with normal pregnant women and GIGT group, this maybe due to the stress during delivery. Insulin levels of most GDM and GIGT were still higher than normal after two month postpartum, and should be followed up carefully.

Adult↗
PubMed1999

Effects of Curcuma Longa on proliferation of cultured bovine smooth muscle cells and on expression of low density lipoprotein receptor in cells.

OBJECTIVE: To investigate the inhibitory effects of aqueous turmeric extract (AqT) and serum of rats orally treated with ethanol extract of turmeric (SeT) on proliferation of vascular smooth muscle cells (VSMC) and its effects on the expression of low density lipoprotein receptor (LDL-R) antigen on the surface of smooth muscle cells. METHODS: Enzyme-linked immunosorbent assay (ELISA) for the expression of LDL-R protein and thiazolyl blue (MTT) assay for the proliferation of VSMC were used in this study. RESULTS: Both aqueous turmeric extract (AqT) and serum of rats orally treated with ethanol extract of turmeric (SeT) could inhibit 10% serum activated proliferation of VSMC. The inhibition shown in both experiments was dose-dependent with an inhibitory rate of 18.9% at 20 mg/ml AqT and rate of 20.1% at 10% SeT respectively. AqT up-regulated the expression of LDL-R protein with a highest rate at 5 mg/ml AqT in 3% lipoprotein deficient serum (LPDS). SeT did not show significant effect on the expression of LDL-R on the surface of VSMC. CONCLUSION: The extracts of turmeric may be extended to decrease the risk of atherosclerosis (AS).

Animals↗
PubMed1999

[Retroviral transfer of a human beta-globin gene linked to beta locus control region hypersensitive site 2 into murine hematopoietic stem cells].

OBJECTIVE: To examine the in vivo properties of retroviral recombinants carrying partially deleted human beta-globin gene (delta beta) and truncated erythroid enhancer (292 bp and 341 bp of 5'HS2) at the mRNA levels following short- and long-term reconstitution in mice with infected marrow cells. METHODS: First ecotropic virus producer cell lines with higher virus titers were isolated using "ping-pong" procedures. Then the human beta-globin gene was transferred into murine hematopoietic progenitor cells and the integration and expression of transferred gene were analyzed by southern blot and RNase protection assay or RT-PCR. RESULTS: The virus titers of both recombinants increased obviously after "ping-pong" procedures. The transferred human beta-globin gene was detected in murine CFU-S12 and the expression level was about 0.5%-5% of endogenous mouse alpha-globin gene. In 3 of 14 mice surviving long-term transplanted with bone marrow cells transduced with high-titer virus, bone marrow, spleen and thymus from two mice and bone marrow and spleen from another mouse contained the intact proviral genome. Long-term expression of the transferred gene was seen in one mouse at level of 7% of endogenous murine alpha-globin gene. CONCLUSIONS: The transferred human beta-globin gene can stably integrate into murine hematopoietic stem cells mediated by retroviral vectors and express in an erythroid-specific manner.

3T3 Cells↗
PubMed1999

[The inhibiting effect of antisense thrombin receptor gene on the proliferation of pig vascular smooth muscle cell].

OBJECTIVE: To search for an effective approach to prevent the formation of restenosis after angioplasty. METHODS: A recombinant eukaryotic expression plasmid vector containing partial antisense thrombin receptor (ATR) gene named pcDNA3/ATR was constructed using recombinant DNA technique. Mini-pig aorta injury model was established by over-sized balloon catheter combined with high cholesterol diet feeding and its aortic smooth muscle cells(ASMC) were cultured. The effect of ATR gene expression in mini pig ASMC proliferation and growth factor gene expression were studied by 3H-TdR incorporation and Northern blot. RESULTS: The DNA synthesis in pig ASMC could be inhibited by ATR gene expression (The DNA synthesis in normal ASMC was lowered by 41.8%, and that in ASMC from injured artery was lowered by (50.3%). The mRNA and protein synthesis of TR could be down regulated by ATR gene expression. The mRNA expression of PDGF-A chain and bFGF stimulated by fetal calf serum (FCS) with thrombin were both downregulated in pig ASMC with expressed ATR gene. CONCLUSION: ATR gene expression can inhibit the proliferation of pig ASMC, and this is induced by its inhibiting effect on TR and finaly the signal transduction in ASMC.

Animals↗
PubMed1999

[Prophylaxis and treatment of cytomegalovirus infection in allogeneic bone marrow transplantation].

OBJECTIVE: To assess the efficacy and safety of Cymevene (ganciclovir) in the prophylaxis and treatment of cytomegalovirus (CMV) infection after allogeneic bone marrow transplantation (allo-BMT). METHODS: Sixty patients with leukemia undergoing allo-BMT were observed. Cymevene was given prophylactically to 20 patients at a dose of 5 mg/kg twice daily from day -9 to day -2 and restarted when the ANC > or = 1.0 x 10(9)/L and BPC > or = 30 x 10(9)/L at a daily dose of 5 mg/kg for 5 days every week until day +100. In diagnosed symptomatic CMV infections, the dose of Cymevene was 5 mg/kg twice daily for 2-3 weeks followed by 5 mg/kg twice a day for 5 days every week for an additional 2-3 weeks. RESULTS: The incidence of active CMV infection was 0 in the prophylaxis-treated patients and 25% in the controls (P < 0.05). In diagnosed CMV diseases, marked improvement was noted in 17 of 20 patients (85%) treated with Cymevene. The most common adverse events of Cymevene were leukopenia and thrombocytopenia, but were reversible. CONCLUSION: Cymevene is effective and safe for the prophylaxis and treatment of CMV infections after allo-BMT.

Acute Disease↗
PubMed1999

[Allogeneic bone marrow transplantation for chronic myeloid leukemia: 118 cases analysis].

OBJECTIVE: To analyze the outcome of allogeneic bone marrow transplantation (allo-BMT) performed for chronic myeloid leukemia (CML) patients. METHODS: One hundred and eighteen CML patients received allo-BMT with conditioning regimens of TBI or modified BU/CY. The pre-BMT situations were, 91 cases in first chronic phase (CP1), 19 in accelerating phase (AP), 2 in blast crisis (BC) and 6 in advanced CP (> CP2). RESULTS: One hundred and nine patients were successfully engrafted. The probabilities of 5 year survival for CP1 and AP patients were 69.6% and 51.0%, and 5 year relapse rates were 3.2% and 12.5%, respectively. CONCLUSIONS: Disease stage, conditioning regimen and splenomegaly did not affect the time for engraftment. For patients in CP1, splenomegaly was positively correlated with relapse. There was no difference between the effect of the two conditioning regimens on the prognosis.

Adolescent↗
PubMed1999

[Efficacy of transcatheter arterial infusion chemotherapy and transcatheter arterial embolization in 132 patients with primary hepatocellular carcinoma].

OBJECTIVE: To find out factors influencing efficacy of transcatheter arterial infusion chemotherapy (TAI) and transcatheter arterial embolization (TAE) for unresectable primary hepatocellular carcinoma. METHODS: From January 1993 to October 1997, 132 patients with unresectable primary hepatocellular carcinoma (HCC) received 597 intervention treatments. Of the 132 patients, 122 patients were given TAI plus TAE (92.4%), while the remaining 10 patients were given TAI alone (7.6%). RESULTS: The 1-, 2-, and 3-year overall survival rate was 81.8%, 36.4% and 18.2%, respectively. The clinical stage of the disease, the embolizing agents used and their dosage, formation of collateral circulation to the tumor and proper application of superselective hepatic arterial catheterization are factors affecting treatment efficacy. CONCLUSION: Adequately performed TAI and TAE can be routinely used for the treatment of unresectable liver cancer.

Adult↗
PubMed1999

[Protective effects of bactericidal/permeability-increasing protein on the vital organ function after endotoxic shock in rats].

OBJECTIVE: To investigate the protective effects of BPI on the vital organ function after endotoxic shock in rats, and to provide experimental evidence for further clinical studies. METHODS: 12.5 mg/kg of E. coli 026:B6 LPS were bolus injected via i.a. to reproduce endotoxic shock. Immediately after injection of LPS, 5 mg/kg of BPI or equal volume of physiological saline were bolus injected via i.a. RESULTS: The survival time of shocked animals was prolonged in the BPI group, 24 h survival rate was higher than that in the saline group. The values of mean arterial pressure, left intraventricular systolic pressure, isovolemic ventricular pressure, and dp/dtmax in the BPI group were markedly higher than those in the saline group. The plasma glutamic-pyruvic transaminase and urea nitrogen levels were obviously lower than those in the saline group. The plasma endotoxin and TNF levels in the BPI group were significantly lower than those in the saline group. CONCLUSIONS: BPI has good protective effects on cardiac, hepatic, and renal function in endotoxic shock in rats, indicating that BPI might be useful in clinic treatment of sepsis/septic shock.

Animals↗
PubMed1999

[External adrenal pheochromocytoma].

OBJECTIVE: To improve the diagnosis of external adrenal pheochromocytoma. METHODS: From 1958 to 1998, 66 cases of external adrenal pheochromocytoma, 23.3% of pheochromocytomas, at the same period in the Department of Urology, RuiJin Hospital were reviewed. RESULTS: In the 66 cases, the malignant rate was high (33%). CONCLUSIONS: (131)I-MIBG is important in the diagnosis and treatment of external adrenal pheochromocytoma. Long-term follow up after operation is imperative to the cases of external adrenal pheochromocytoma.

3-Iodobenzylguanidine↗
PubMed1999

[A clinico-pathological and etiological study of Binswanger's Disease].

OBJECTIVE: To study the pathology, incidence and etiology of Binswanger's disease. METHODS: Autopsied brains from 9 cases of Binswanger's disease and 13 cases of non-demented elderly individuals were studied by clinico-pathological, immunohistochemical and micrometer methods. RESULTS: The lesions of Binswanger's disease were mainly located in the subcortical white matter, periventrical regions, brainstem and cerebellum. The pathological changes were demyelination, lacunar infarction, gliosis and dilatation of perivascular space (Virchow-Robin space, VR space, P < 0.01). The wall thickness of deeply penetrating arteries in the white matter was significantly increased (P < 0.01). CONCLUSION: Binswanger's disease is not a rare entity. The findings of this study suggest that arteriosclerosis is a primary factor in the pathogenesis of Binswanger's disease.

Aged↗
PubMed1999

[Pathomorphological and amyloid beta-protein immunohistochemical findings in autopsied brains of Alzheimer's disease].

OBJECTIVE: To study the incidence and neuropathological features of Alzheimer's disease in Chinese and the significance of silver and amyloid beta-protein staining for the pathological diagnosis of Alzheimer's disease. METHODS: Among 445 autopsy cases above 60 years in age (including 60) collected from 1982-1998 in the Department of Pathology, Beijing Hospital, totally 29 cases were diagnosed as Alzheimer's disease pathologically (6.5%), accompanying with a history of mental deterioration and the histological changes in certain areas of the brain fulfilling the Khachaturian or Mirra criteria. Tissue blocks were taken from 6 standard regions of the brains for Bodian, Bielschowsky and amyloid beta-protein immunostaining. Senile plaques and neurofibrillary tangles were quantified. RESULTS: Four main types of senile plaques were noticed and the result by amyloid beta-protein immunostaining was considered more sensitive than that of the silver impregnation staining. The former one enabled to demonstrate all the 4 types of senile plaques, while silver impregnation staining could only demonstrate clearly the primitive and neuritic plaques, but not the small diffuse and burn-out plaques. The senile plaques were noticed to be focused in the hippocampus, superior temporal gyrus and middle frontal gyrus while the neurofibrillary tangles, were mainly obtained in the hippocampus, temporal cortex and amygdaloid. Cerebral amyloid angiopathy was found in 25 out of 29 Alzheimer's disease cases. CONCLUSIONS: Alzheimer's disease may be the main cause of dementia among the elders in Chinese and immunostaining for amyloid beta-protein pretreated with formic acid may set a high value on the pathological diagnosis of Alzheimer's disease.

Aged↗
PubMed1999

[Affect of insulin-like growth factor I and estradiol on the growth of uterine leiomyoma].

To study roles of insulun-like growth factor 1(IGF-I) and estradiol(E2) in the growth of leiomyoma. Protein expressions of IGF-I receptor(IGF-I-R), estrogen receptor(ER), and cell proliferation associated nuclear antigen(ki-67) were analysed by streptavidin-peroxide method in 40 cases. Serum level and local concentration of IGF-I were determined by immunoradiometric assay(IRMA) in 20 cases. Serum level of estradiol was determined by radioimmunoassay in 20 cases. Twenty normal fertile women were studied as control. Protein expressions of IGF-I-R and ER in uterine leiomyoma were significantly higher than in myometrium(P < 0.05); and local concentration of IGF-I positively correlated with ki-67 (r = 0.6513, P < 0.05); and expression of estrogen receptor. The serum levels of IGF-I and E2 had no significant difference between leiomyoma patients and normal women. IGF-I and E2 promote the growth of leiomyoma and IGF-I may act as a mediator of estrogen.

Adult↗
PubMed1999

[A study on chemical components of Tetrastigma hemsleyanum Diels et Gilg. Native to China].

OBJECTIVE: To investigate the chemical constituents of Tetrastigma hemsleyanum native to China. METHOD: Compounds were separated by column chronatography with silica gel and elucidated by chemical evidence and spectral analysis. RESULT: Four compounds were isolated from the petroleum ether extract. Their structures were identifies as taraxerone, taraxerol,beta-sitosterol and ergosterol. CONCLUSION: These compounds were isolated from the plant for the first time.

Ergosterol↗
PubMed1999

[Studies on pharmacological junctions of hairy root of Astragalus membranaceus].

OBJECTIVE: To investigate the effect of hairy root of Astragalus membranaceus(HRA). METHOD: HRA were given 10 g/kg per day for 50 days to aged mice treated with D-galactose, and the effect on memory and antioxidant functions were estimated. After administration of HRA 10 g/kg for four days, anti-ischemia-reperfusion kidney model of rat was prepared. The kidney function, activity of superoxide dismutase (SOD) and content of malondialdehyde(MDA) in kidney were examined. Mice with immunosuppression induced by cyclophosphamide were given orally HRA 10 g/kg for 12 days. The activity of natural killer (NK) cells was measured. RESULT: HRA improved the memory, raised SOD activity in brain and liver, decreased the MDA content in the liver of aged mice, reduced the MDA content in ischemia-reperfusion kidney, decreased the creatinine level in blood of rats, and promoted the activity of NK cells in immunosuppressed mice. CONCLUSION: Similar to the natural A. membranaceus, HRA has senility-preventing, antioxidizing and immunomodulating functions.

Aging↗
PubMed1999

[Effect of enoxacin on pharmacokinetics of theophylline in rats].

In order to obtain an experimental evidence for Enoxacin(ENX) to be correctly used in clinical treatment, we studied the effect of ENX on the pharmacokinetic parameters of theophylline(TP). A single oral dose of TP 20 mg/kg was given to rats and ENX(300 mg/kg, 450 mg/kg) was co-administered orally three times to those rats. The plasma concentrations of TP were determined by HPLC after TP was administered 1, 2, 3, 5, 7, 12 and 24 hrs. The results showed that TP was eliminated by one compartment model. TP plasma concentrations and AUC were significantly increased. T1/2 beta of TP was prolonged. The total clearance of TP was decreased when compared with the control. This interaction was dose-dependent. It was concluded that the interaction between ENX and TP existed. Concomitant use of ENX with TP should be avoided.

Animals↗
PubMed1999

[Rapid method for determination of chlordimeform residue in honey].

In the present work. TLC was used as a rapid method for determination of chlordimeform in honey. Twenty gram of sample was extracted by n-hexane in alkali solution, re-extracted by acidic aqueous solution, then hydrolyzed in NaOH solution for 10 min on boiling water bath. The hydrolyzed products were extracted by methylene chloride. After passing through the Na2SO4 column for dehydration, the methylene chloride should be evaporated to less than 70 degrees C to 0.5 mL spot 50 microL sample solution on the silica gel plate, develop the plate about 8 cm from the surface of developing solvent V(benzene):V(chloroform):V(ethyl acetate) = 5:5:1. The plate was kept in the atmosphere (NaNO2 + HCl) for 10 min and 5% N-(1-naphthyl) ethylendiamine dihydrochloride was sprayed on it. Put it into a dark room for 20 min and then observe and estimate the amount of chlordimeform in the sample. The limit of determination is 10 ng. The experiment could be completed in 3 h for a sample without large equipment and special reagent.

Chlorphenamidine↗