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Biomedical subjects

D Lee

Publications and source records attributed to D Lee.

At least 631 records · Page 35Linked to original sources
PubMed1977

Incorporation of galactose into galactosyltransferase.

Bovine skim milk galactosyltransferase (EC 2.4.1.22) retained its catalytic activity after partial enzymatic removal of sialic acid and galactose. Desialylated and degalactosylated galactosyltransferase was a galactosyl acceptor in the galactosyltransferase reaction. [14C]Galactose from UDP-[14C]galactose was incorporated into the carbohydrate-depleted galactosyltransferase by native galactosyltransferase. The results suggest that galactosyltransferase participates in the biosynthesis of its glycopeptides of the sialic acid-galactose-N-acetylglucosamine type.

Animals↗
PubMed1977

A student-run course in interprofessional relations.

The topic of working relationships among health professionals is one which has long been neglected in professional school curricula. During the winter of 1976 students from four health professions--medicine, nursing, occupational therapy, and physical therapy--initiated a course designed to improve their knowledge of one another's training and skills, with the larger aim of preparing themselves to work in health care teams. The nine weekly meetings are described in terms of their content and students' reactions. In the discussion the underlying themes of legal responsibilities and of sex-roles as factors in health care team functioning are identified. Suggestions are made about format and techniques that seem to be useful for courses of this type.

Education, Medical, Undergraduate↗
PubMed1977

Maintenance of the functional state of isolated rat liver by hypothermic perfusion with an erythrocyte-free medium.

Isolated rat liver was studied before, during, and after hypothermic perfusion at 5 C for 24, 48, or 72 hr with an acellular perfusate consisting of 7% bovine serum albumin in Kreb-Ringer buffer containing glucose, penicillin, and streptomycin. Bile production ceased at 5 C but resumed when the temperature was raised to 35 C. The rate of flow and the total amount produced was unaffected by 24 hr of hypothermia but decreased when the cooling period was extended to 48 and 72 hr. The data of other workers was used to show a correlation between bile flow and oxygen consumption by the liver. Cooling also caused the release of potassium into the perfusate but it was quickly reaccumulated after rewarming; however, the extent and rate of reaccumulation decreased as the cooling period increased, as did the ability of the livers to retain the ion. Urea synthesis did not cease after cooling and after rewarming, the rate of synthesis increased as the period of hypothermia was lengthened. The maximum concentration of urea in the perfusate was found when rewarmed livers had produced 200 mumol of urea but at this point, control livers had produced 280 mumol. The concentration of glucose in the perfusate of livers maintained at 35 C showed peaks at 2 and 9 to 10 hr after the start of perfusion. After cooling for 24 hr these peaks arose at 2 and 7 hr after rewarming, but with 48 hr of hypothermia, these peaks were higher and appeared at 2 and 4 hr. When the cooling period was extended to 72 hr, only a single peak was seen 2 hr after rewarming. These results suggest that rat liver can be cooled to 5 C for 24 hr with little effect on its functional characteristics but a marked decline becomes apparent when the cooling period is extended beyond 24 hr. None of the livers studied was transplanted after perfusion and it remains to be seen how the functional tests conducted in vitro correlate with the ability of the livers to support life.

Animals↗
PubMed1977

The effect of temperature on the synthesis of rhinovirus type 2 RNA.

The reduced yields of rhinovirus type 2 at temperatures above 37 degrees C were shown to result from the degradation of virus-induced RNA, leaving little RNA available for inclusion into mature infectious virions. The degradation occurred about 6 h p.i., and appeared to be selectively effecting the single-stranded species. Lysosomal nucleases do not appear to have a role in this supra-optimal degradation.

RNA, Viral↗
PubMed1977

The use of IgM anti-D coated cells in the deliberate immunisation of Rh-negative male volunteers.

Attempts to produce anti-D in rhesus-negative men using reconstituted frozen rhesus-positive cells were unsuccessful. The use of cells coated with IgM anti-D for immunisation stimulates anti-D production, and a comparison between two groups, one having cells coated with IgM anti-D, and the other having identical cells without coating suggests that there is an enhancement of the immune response when IgM-coated cells are used.

Antibody Formation↗
PubMed1977

The effect of colloid concentration on the functional characteristics of isolated perfused rat liver.

The volume of bile produced by isolated perfused rat liver was dependent on the concentration of bovine serum albumin (BSA) in the perfusate, the lowest volumes being obtained with Krebs Ringer bicarbonate (KRB alone and with 70 g/l. BSA in KRB. Increasing amounts were produced as the concentration of BSA was reduced from 70 g/l. to 8.5 g/l. The concentration of bile salts in the bile decreased rapidly during the first hour, but much more slowly thereafter. The highest concentration observed during the first hour was with KRB alone and the lowest with 20 g/l. BSA; subsequently, the highest concentrations were obtained with 70 g/l. BSA and the lowest with 20 g/l. BSA. Urea synthesis was lowest with KRB alone, but increased as the concentration of BSA increased; however, values obtained with 8.5, 20, and 40 g/l. were fairly close together. With KRB alone there was a rapid release of potassium during the first hour, but thereafter the rate of release was similar to that found when BSA was present. The lowest concentrations of potassium were obtained with 20 and 40 g/l. BSA. With KRB alone, GOT was released from the start of perfusion of 300 units per minute. With BSA present, GOT release was delayed 4 hours, and thereafter the rate was dependent on the concentration of BSA, being lowest with 20 and 40 g/l. (200 units per min), higher with 8.5 g/l. (350 units per min) and highest with 70 g/l. (400 units per min).

Animals↗
PubMed1977

Comparison of nucleic acid content in populations of free-living and symbiotic Rhizobium meliloti by flow microfluorometry.

Populations of symbiotic Rhizobium meliloti extracted from alfalfa nodules were shown by flow microfluorometry to contain a significant number of bacteroids with higher nucleic acid content than the free-living rhizobia. Bacteroids with lower nucleic acid content than the free-living bacteria were not detected in significant quantities in these populations. These results indicate that the incapability of bacteroids to reestablish growth in nutrient media may not be caused by a decrease in nucleic acid content of the symbiotic rhizobia.

DNA, Bacterial↗
PubMed1976

Primary repair in transection of duodenum with avulsion of the common duct.

In combined pancreatoduodenal trauma, the surgeon usually chooses a diverting or resectional procedure at substantial risk. Simple closure of the duodenum and transposition of the common duct was accomplished at early operation in an 18-year-old boy who had almost complete transection of the duodenum with avulsion of the ampulia of Vater from blunt abdominal trauma. Transgastrojejunostomy and use of Chaffin tubes were discontinued prior to discharge from the hospital three weeks later. The postoperative course was complicated only by a brief pancreatitis. The excellent result persists more than two years later. In similar anatomic situations, consideration might be given to this operative approach.

Abdominal Injuries↗
PubMed1975

Studies on the conditions required for optimum recovery of Tetrahymena pyriformis strain S (Phenoset A) after freezing to and thawing from -196 C.

The toxicity of several cryoprotective agents was tested at room temperature (23 C) against Tetrahymena pyriformis strain S (Phenoset A) at different stages of growth cycle. Polyvinylpyrrolidone (PVP-40) at 10 per cent (v/v) concentration was without effect at any stage in the growth cycle, while 1.2 M glycerol immobilized the cells which were disrupted very shortly afterwards. The toxicity of 0.25 M glucose was largely independent of the position of the cells in the growth cycle, but the toxicity of 0.25 M sucrose and 1.4 M dimethylsulfoxide (DMSO) was most marked in late log- and stATIONARY-PHASE CELLS. After log-phase cells had been equilibrated with 1.4 M DMSO for 1 hr, the number of cells surviving cooling at defined rates from 0.45 to 12 C/min decreased as the final temperature decreased from -30 to -60 C. A temperature of -53 C was found to be the optimum from which cells cooled at a given rate could be cooled rapidly to -196 C. Nevertheless, when cells were cooled at defined rates to -35, -45, or -53 C and then rapidly to -196 c the optimum rate of cooling to these temperatures was found to be 1 C/min. The optimum rate of cooling to -60 C prior to plunging into liquid nitrogen was found to be 2.7 C/min.

Cryoprotective Agents↗