Biomedical subjects

D Lazarević

Publications and source records attributed to D Lazarević.

13 recordsLinked to original sources

PubMed2005

Discovery of 342 putative new genes from the analysis of 5'-end-sequenced full-length-enriched cDNA human transcripts.

In this work we describe the process that, starting with the production of human full-length-enriched cDNA libraries using the CAP-Trapper method, led us to the discovery of 342 putative new human genes. Twenty-three thousand full-length-enriched clones, obtained from various cell lines and tissues in different developmental stages, were 5'-end sequenced, allowing the identification of a pool of 5300 unique cDNAs. By comparing these sequences to various human and vertebrate nucleotide databases we found that about 40% of our clones extended previously annotated 5' ends, 662 clones were likely to represent splice variants of known genes, and finally 342 clones remained unknown, with no or poor functional annotation. cDNA-microarray gene expression analysis showed that 260 of 342 unknown clones are expressed in at least one cell line and/or tissue. Further analysis of their sequences and the corresponding genomic locations allowed us to conclude that most of them represent potential novel genes, with only a small fraction having protein-coding potential.

5' Flanking Region↗

PubMed2004

[Correlation of diagnostic procedure with surgical findings in carotid stenosis].

Authors are discussing dilemma about necessary diagnostic procedure in making decision for surgical treatment of the carotid disease. Attitudes are changing and the old opinion of the necessity for angiogram of extra cranial blood vessels is substituted by the opinion that CDS (Color Duplex Scan) is satisfactory in the majority of cases to indicate of the surgical treatment. Comparing the invasive and noninvasive diagnostic's with operative findings, authors empirically confirm the given hypotheses, looking back to the cases where angiogram is necessary. The following issues were discussed: Estimate of the locaton of maximal shrinking, degree of shrinking, length of shrinking and characteristics of plaque as the most important parameters for indication and operative tactics. Diagnostics with CDS is shown as reliable with high degree of matching with operative findings.

Adult↗

PubMed1999

[A scoring system for polytrauma patients].

The paper presents a review study on score systems in current emergency surgery including its classification, application and value, based on the experiences of other authors, the paper suggests application of score systems in different phases of polytraumatized patients treatment (in prehospital, as well as in early and late phases of the inpatient period). The paper also points out certain objective difficulties in everyday scoring of patients at the Department of the Emergency Surgery (daily engagement, computer center, etc.). As a conclusion, the authors present their opinion indicating that in absence of introduction of these systems into the practice, the approach to a polytraumatized patient cannot be adequate.

Humans↗

PubMed1998

A novel p53-inducible gene coding for a microtubule-localized protein with G2-phase-specific expression.

Wild-type (wt) p53 can act as a sequence-specific transcriptional activator and it is believed that p53 elicits at least part of its biological effects by regulating the expression of specific target genes. By using a differential subtractive hybridization approach in a murine cell line stably transfected with a temperature-sensitive p53 mutant (Val135), we isolated a set of genes markedly induced by wt p53. One of them, provisionally named B99, was further characterized; its transcriptional induction was dependent on wt p53 function and the corresponding protein product was shown to accumulate after DNA damage in different cell types. Immunofluorescence analysis located the B99 protein to the microtubule network. Flow cytometry revealed that upon activation of p53 function the endogenous B99 protein was selectively induced in the G2 fraction of the cell population. When B99 was ectopically expressed in p53-null murine fibroblasts, B99-transfected cells displayed an increased fraction with a 4N DNA content, indicative of interference with G2 phase progression. Taken together these data suggest that B99 might play a role in mediating specific biological activities of wt p53 during the G2 phase.

Amino Acid Sequence↗

PubMed1989