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Biomedical subjects

D Laky

Publications and source records attributed to D Laky.

At least 127 records · Page 7Linked to original sources
PubMedDate not supplied

Retroperitoneal malignant fibrous histiocytoma without histological atypias.

A case of a 36-year-old woman suspected of hepatic hydatid cyst with dyseptic symptoms, hepatomegalia, profound pains in the right hypochondrium is presented. During the operation a 15/12/17 cm well-incapsulated tumour is extirpated. The tumour stretched from the upper right renal pole behind the right hepatic lobe as far as the diaphragm and while sectioning it was grey and orange. Histopathologically we found a fibrous histiocytoma with zones of storiform fascicular pattern alternating with areas of histocytic cells with vacuolar-foamy cytoplasm, rich in lipids and without atypias or mitoses. We report the frequency, histological aspects of the fibrous histiocytomas as well as some particularities of their retroperitoneal location.

Adult↗
PubMedDate not supplied

Mammary carcinosarcoma with osteochondrosarcomatous differentiations.

The case of a 77-year-old woman with a 6/5/4.5 cm, mobile, hard, white-yellowish tumour associated with axillary adenopathy, with a progressive evolution in the upper external frame of the right breast is presented. The histopathological examination, carried out after the radical mammectomy, revealed zones with plates of slightly differentiated carcinomatous cells forming keratose pearls next to dense mixofibrochondrosarcomatous proliferations. The scarcity of these tumors as well as the mammary sarcomas and histogenesis questions are discussed.

Aged↗
PubMedDate not supplied

Voluminous solitary non-Hodgkin extraganglionary lymphoma of thigh.

The case of a 36-year-old woman is presented. She had a voluminous tumour inside the 1/3 average part of the right thigh at 12 cm from the inguinal region. Apart from some local pains, she had a normal haematological formula, normal spleen, liver and lymph nodes. As the tumour of 5.5 x 5 x 4.5 cm was well incapsulated between the sartorius muscle and the great adductor tendon it was easily extirpated and presented a whitish-light yellow section. The histological cellular aspect was pleiomorphic with round, ovalar cells; some were big and clear cells of centroblast-type with several mitoses and giant multinucleated cells and collagenic bundles in between. The nodular Hodgkin sclerosis is not confirmed immunohistochemically because of the negative reaction for antigen Kil (CD30) so that the diagnosis was lymphoma with lymphoma with big B cells by CD20 reaction. We also found positive T cells at CD43 that might be reactive lymphocytes. The tumour ranks a rare malignant lymphoma and for the first time, an extraganglionar lymphoma in thigh was found. We have not met such a location in the literature.

Adult↗
PubMedDate not supplied

Histoenzymological and ultrastructural research of myocardial mitochondria in cardiac insufficiencies.

We performed histoenzymological and electron-microscopic investigations of 40 myocardial biopsies taken during open heart surgery under extracorporeal circulation (ECC) from patients with valvular cardiopathies and septal interatrial defects with various degrees of congestive cardiac insufficiency (CCI). The changes occurring in the topochemistry and intensity of the enzymatic reactions used as markers for mitochondria, namely SDH and LDH, demonstrated the existence of a correlation between the decrease of reactions of the CCI degree. The ultrastructural aspects of the mitochondrial changes attested the precocity and severity of damage in these organelles even from initial moments of hypoxia.

Cardiac Output, Low↗
PubMedDate not supplied

Histopathological and ultrastructural modifications of the articular cartilage in ankylosing spondylarthritis.

The present study aims to analyse the articular cartilage in Ankylosing, Spondylarthritis (AS) versus the normal cartilage (18 cases altogether) by histopathological (HE, VG, Pas-Alcian, Gömöri, Safranine O), electronmicroscopic and histoenzymological techniques starting from the finding that the articular exudate in this affection is characterized cytomorphologically by cytosis (6,100/mm3), with polynucleosis (61%) and ragocytosis (25%). Histopathologically, the articular cartilage in AS evinced an alteration of safraninophilic areas and pale zones located in the neighbourhood of synovial lymphoplasmocytic infiltrates, pointing to the depletion of proteoglycans. The cartilage-synovia interference areas are relevant for the invasive nature of the inflammatory infiltrates which erode the cartilage. The histopathological modifications were confirmed ultrastructurally by the existence of some extended areas of revealed collagen, indicative of proteoglycans depletion. Functionally, chondrocytes have a high oxidative potential expressed by the presence of the peroxisomes and the positive reaction of peroxidase (PO), located near the nuclear membrane and the rough endoplasmic reticulum. The corroboration of histopathological, ultrastructural and histoenzymological data shows the alteration of the articular cartilage in AS, expressed morphologically at the level of the chondrocytes and of their synthesis products, collagen and proteoglycans.

Cartilage, Articular↗
PubMedDate not supplied

Carcinosarcoma of the kidney (nephroblastoma in an adult). Case report.

There was reported a case of a 57-year-old man with right renal colics and haematuria for the past 12 years, supposed to be caused by renal cysts and pyelonephritis. The latest echographs and functional explorations show an expanded right kidney with frequent cysts. The extirpated kidney is about 12/15 cm in size and has an encephaloid tumour in the pyelocalycial areas penetrating in the surrounding parenchyma. The histological picture is that of a carcinosarcoma, showing a possible origin in the restant multipotent cells of the nephrogenic blastoma.

Carcinosarcoma↗
PubMedDate not supplied

Infantile spinal amylotrophy Werding-Hoffman disease and congenital contractures.

The authors present a rare case of a male infant aged 5 1/2 months with muscular contractures, hypotonia and areflexia. Two male siblings died with the same clinical picture. The microscopic findings of several spinal cord specimens are typical for Werding-Hoffman amylotrophia spinalis. The microscopical aspects are suggestive for neurogenic atrophy of muscular fibres with interstitial myositic process. It is proposed to include the present case in X-linked form of infantile spinal muscular atrophy.

Contracture↗
PubMedDate not supplied

Leiomyoosteochondroma of the stomach. A case report.

The case of a 65-year-old man with a large 6-year gastric tumour revealed echographically is reported. A well delimited consistent white-grey tumour occurring in the anterior wall of the stomach and projecting from the gastric serosa in the subhepatic region was surgically found. Histologically, it showed leiomyomatous zones with fascicular spindle cells and sometimes with perinuclear vacuolizations that are actin-reaction positive but protein S-100-negative. These zones alternated with large compact benign osteochondromatous ones. The uncertain origin of this uncommon tumour was discussed. We have not found any references to such gastric tumours.

Aged↗
PubMedDate not supplied

Electronmicroscopic and histoenzymologic study regarding morphophysiopathologic visceral modifications during open heart operations under extracorporeal circulation.

Morphophysiopathologic modifications occurred in myocardium, liver and lung during open heart operations under extracorporeal circulation had been studied using histologic, histoenzymologic, ultrastructural and biochemical investigations. Preexisting myocardic and hepatic lesions subsequent to chronic stasis and hypoxia are accentuated due to the "controlled shock" state induced by extracorporeal circulation. Mitochondrial marker enzymes like SDH, LDH and ATP-ase in the membrane are decreasing their levels and hydrolytic enzymes like acid phosphatase, nonspecific esterase (ANAE) and peroxidase are increasing their activity. These disseminated lesions can become irreversible under the conditions of a severe cardial insufficiency and of a prolonged operation. In the lung, the increase in the hydrolytic enzyme activity suggests the processes of phagocytosis and metabolization of red cells by macrophages.

Adenosine Triphosphatases↗
PubMedDate not supplied

Gastrointestinal stromal tumors--a recently defined entity. Literature data and personal case report.

Stromal tumors of the gastrointestinal tract are controverted tumoral entities which were recently delimited. They were initially identified by immunohistochemical investigation and processing of the gastrointestinal muscular tumors and of the malignant and benign nervous tumors. Those investigations were subsequently amplified by electronmicroscopic studies and observations during the patient's prognostic follow-up. Within these circumstances, monoclonal tumors responsive to smooth muscular antigens (actin, SMA), to polyclonal antigens reacting to S-100, PGP 9.5, NSE and GEAP were identified. Thus, tumors with a nervous origin known as a gastrointestinal nerve tumors or plexosarcomas were differentiated, taking into account the difficulty of their distinguishing from gastrointestinal tumors (GIST) which also shows multinucleated great cells. There were also difficulties in defining bidirectionally differentiated tumors or those nonresponsive to any antigen, all these elements proving their origin from nondifferentiated mesenchymal cells. These data are presented in a personal case report of a 28-year old woman with an acute anteroenteral invagination due to a gastrointestinal stromal tumor, favorably removed by surgery and with a postoperative outcome.

Adult↗
PubMedDate not supplied

Immunocytomorphopathological studies on the pathogenesis of rheumatoid arthritis.

Thirty cases of rheumatoid arthritis were submitted to cytomorphological, histopathological (HE, VG, PAS Alcian, Gömöri, Safranine O), histoenzymological (Acid Phosphatase, chondroitin-sulphatase, Peroxidase) and immunological (rheumatoid factor (RF)) studies; circulating immune complexes, anti-collagen antibodies II, Reactive C protein (CRP), Complementary C3 fraction were also assessed. The synoviocytogram of the rheumatoid synovial fluid (SF) indicated a cytosis with polynucleosis and ragocytosis compared to the hydroarthrosic SF defined by lymphocytosis (47.8%). Enzymologically, especially for high titres of rheumatoid factor, a phosphatase and peroxidase activity was observed in polymorphonuclear cells of a ragocytary type and in phagocytic mononuclear cells. The severe forms of rheumatoid arthritis (RA) were correlated histopathologically with chronic villous synovitis associated with some processes of obliterant vascularitis, fibrosis and sclerosis. At the level of synovio-cartilage junction, fissures and a homogenization of the cartilaginous fundamental substance in the vicinity of disintegrated synovial structures were noticed. Histoenzymologically, a lysosomal and oxidative activity was found in chondrocytes and in synovial macrophages. Immunological assessments (73 serum and 60 synovial fluid samples) showed pathological values of circulating immune complexes, anti-collagen antibodies and C reactive protein. The complementary synovial depletion of the C3 fraction underlines the immune character of the rheumatoid synovitis. The immunocytomorphologic data correlation demonstrates the involvement of immunologic and enzymatic factors in the evolution of Rheumatoid Arthritis.

Acid Phosphatase↗
PubMedDate not supplied

Anatomoclinical and bacteriological study on chronic marginal periodontal disease. Therapy with staphylococcic vaccine.

Histological, histochemical and electronmicroscopic investigations were carried out on gum biopsies taken from cases with chronic marginal periodontal disease before and after the treatment with staphylococcic vaccine, thus, morphopathologically proving the findings of Gafar et al. (1985) and of Georgescu et al. (1973; 1974; 1975; 1979; 1995). Before treatment, gum lesions were severe, consisting in an abundance of polymorphic inflammatory infiltrates in the chorion along with oedema and disorganised connective fibres, atrophies and ulceration of the mucosa, purulent deposits at the surface with bacteria and fungi. After the treatment with the staphylococcic vaccine, an epithelial regeneration was seen together with the absence of the chronic inflammation. The intensely fibroparius granulated tissue was also noted, in all cases observing abundant connective fibres, accounting for good results after treatment, notably the firmness of the tooth fitting.

Bacteriological Techniques↗
PubMedDate not supplied

Morphopathological and clinical diagnosis aspects of a bilateral dysembryoplasic renal tumor associated with Bourneville's tuberous sclerosis.

A 34-year old woman was admitted for bilateral flank pain, maleolary and palpebral oedema with an insidious evolution lasting for 5 years. Urogram showed a left ureteral duplicity and a right lacunary pelvic image. MRI and echography indicated the complete modification as a renal structure as well as the presence of some bilateral tumoral formations with a nonhomogenous structure, blood diffusion and adenopathy of the renal hilum. Nephrectomy revealed white-gray tumoral nodular tumours. The histopathological test evidenced proliferations of leiomyofibromatous, angiomatous, lipomatous, cartilaginous types with a benign aspect suggesting the diagnosis of a renal dysembryoplasic tumor (hamartoma).

Adult↗
PubMedDate not supplied

Morphophysiopathologic studies on the biology of myocardium in acquired valvulopathies.

Myocardial lesions due to various valvulopathies were sampled during open heart surgery under extracorporeal circulation, and histologically, histoenzymologically and ultrastructurally investigated. Various cardiomyocitary and interstitial nonspecific progressive lesions, due especially to hemodynamic and hypoxic alterations, were identified. During the pathogenic stages, we remarked the onset and chronic evolution of perivascular and interstitial oedema, enhanced by a reduced lymph draining. The progressive processes of hyalinization, sclerosis and interstitial fibrosis were subsequent to the interstitial matrix modification. These processes accentuate the myocardial hypoxic lesion due to the nutritional and gaseous exchange alterations. The infrastructure, mitochondrial enzyme equipment, sarcoplasma and tubular network lesions, as well as the intramyocitary oedema that destroys the sarcomeric structure, ended with the activation of lysosome and lysosomal enzymes, giving rise to consequent cytolytic foci. Therefore, the process was extending. In this context, we remarked at the electronmicroscopic examination ventricular natriuremic granules, that are rarely mentioned in literature.

Adult↗
PubMedDate not supplied

Retroperitoneal extraadrenal paraganglioma report of two cases.

Two cases with retroperitoneal tumours affecting young women who cannot undergo surgery were investigated by biopsy and needle sampling. One of these cases presented a mainly alveolar histologic pattern and was easily diagnosed by immunohistochemical means, as well as a chromaffin paraganglioma. We discussed different diagnoses and approaches in the literature.

Adult↗
PubMedDate not supplied

Electronmicroscopic observations regarding the presence of natriuretic granules in the ventricle of patients with cardiopathies.

As part of studies regarding the human myocardium biology in hereditary and acquired cardiopathies, we showed the presence of natriuretic granules (NG) not only in atriums but also in ventricles, in ten of our cases with intraoperative myocardium biopsies. Ultrastructurally, the natriuretic granules occur in lesions produced by hemodynamic and consecutively hypoxic disturbances, being present at the ventricular level, too, both near the Golgi apparatus that secrets them, and diffusely, in cardiomyocytes, beneath the altered organelles and phagolysosomes. Their aspect in similar in cardiac malformations (DSIA. Fallot tetralogy) and in mitral valvulopathies, their abundance being in connection with congestive heart failure (CHF). Although predominant in CHF class I and II, they occur also in the severe decompensations class III and IV, being perhaps felt at the cardiac level as they are released in the blood. This phenomenon is expressed by electronmicroscopic presence of natriuretic granules in the subsarcolema and the increased plasma level of natriuretic peptide, according to biochemical findings reported in the literature. They produce a vasodilating, diuretic, natriuretic effect, contributing to blood pressure regulation and testifying in the neuroendocrine role of human myocardium.

Atrial Natriuretic Factor↗