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Biomedical subjects

D L Wilson

Publications and source records attributed to D L Wilson.

At least 127 records · Page 7Linked to original sources
PubMed1981

Evaluation of an indirect hemagglutination test for Legionella pneumophila serogroups 1 to 4.

Parallel testing of 895 sera by indirect hemagglutination and indirect fluorescent-antibody techniques showed 97.3% agreement. Although the indirect hemagglutination technique usually showed more cross-reactivity among serogroups than the indirect fluorescent-antibody technique with Formalin-fixed antigens and a conjugate which detected primarily immunoglobulin G antibodies, heterologous serogroup reactions were significantly lower than homologous serogroup titers and the etiological serogroup could be easily defined. The indirect hemagglutination techniques showed no cross-reactivity with a crude extract of Escherichia coli O13:K92:H4. Since the indirect hemagglutination technique was shown to detect both immunoglobulin M and immunoglobulin G antibodies and was found to be rapid, simple, and inexpensive, it appears to be an excellent alternative to the indirect fluorescent-antibody test for serodiagnosis of legionellosis.

Antibodies, Bacterial↗
PubMed1981

Delayed auditory brainstem responses in diabetes mellitus.

Diabetic patients have longer interpeak latencies in the brainstem auditory evoked responses than age-matched controls. The delay is not related to clinical hearing loss or blood glucose level at time of testing. Since waves I and II are normal in latency, the conduction velocity of the eighth nerve is not involved. The delay occurs between waves II and V, which would reflect altered transmission times in auditory brainstem and midbrain structures, and suggests the presence of a central neuropathy in patients with diabetes.

Adult↗
PubMed1979

Qualitative analysis of proteins rapidly transported in ventral horn motoneurons and bidirectionally from dorsal root ganglia.

Two-dimensional electrophoresis has allowed a higher-resolution comparison of rapid transport in ventral horn motoneurons and bidirectionally in dorsal root sensory neurons. Dorsal root ganglia 8 and 9, or hemisected spinal cords, from frog were selectively exposed in vitro to 35S-methionine. Transported, labelled proteins that accumulated in 3 mm segments proximal to ligatures on dorsal roots and spinal nerves or sciatic nerves were subjected to two-dimensional gel electrophoresis. Comparisons were made of fluorographic patterns from dried gels. Sixty-five species of proteins were found to be rapidly transported in both bifurcations of dorsal root sensory neurons. No abundant species of protein was rapidly transported in dorsal roots that was not also found in spinal nerves. A comparison of proteins rapidly transported in the sciatic nerve from ventral horn motoneurons with those from dorsal root sensory neurons yielded 50 common species of polypeptides. At most four minor species were possibly transported only in ventral horn motoneurons. An overall comparison indicates that at least 45 species of proteins, including all of the more abundantly transported ones, were consistently common to both dorsal root bifuractions and to ventral horn motoneurons. This appears to be the case despite the very different functions carried out by motoneurons and sensory neurons.

Animals↗
PubMed1979

False-positive reactions in the rapid plasma reagin-card, fluorescent treponemal antibody-absorbed, and hemagglutination treponemal syphilis serology tests.

Sera from 628 nonsyphilitic individuals were tested with the Rapid Plasma Reagin-Card, Fluorescent Treponemal Antibody-Absorbed, and Hemagglutination Treponemal Test for Syphilis tests to ascertain the comparative specificity of these tests. Many sera were also tested with the quantitative Venereal Disease Research Laboratory test. Sera included in the study were from both normal individuals and patients with a variety of illnesses and conditions. The Hemagglutination Treponemal Test for Syphilis gave the lowest overall percentage of false-positive reactions (1.6%), followed by the Fluorescent Treponemal Antibody-Absorbed test (3.3%) and the Rapid Plasma Reagin-Card test (10.8%).

Adult↗
PubMed1979

Prevalence of antibodies to Legionnaires' disease. A seroepidemiologic survey of Michigan residents using the hemagglutination test.

The indirect fluorescent antibody test and a microhemagglutination technique detected antibodies in human sera to Legionnaires' disease antigen about equally. Since the hemagglutination technique was simpler, more rapid, and less expensive, we used it to ascertain the prevalence of antibody in 1200 sera from apparently healthy, nonhospitalized Michigan residents. Prevalence was analyzed by age, sex, geographic location (county or residence), and season of the year. There was a significant difference in prevalence between seasons: 91 of 600 sera (15.2%) from February to March 1978 had a titer equal to or greater than 1.16, contrasted with 179 of 600 sera (29.8%) from August to September 1978. This difference was independent of age and sex. There was no significant difference by geographic location, sex, or age except for decreased prevalence for persons 60 years or older.

Adolescent↗
PubMed1978

Two-dimensional gel electrophoresis of proteins in rapid axoplasmic transport.

Two-dimensional electrophoresis of proteins has allowed high resolution analysis of the protein species rapidly transported in the frog sciatic nerve. The 7th, 8th and 9th dorsal root ganglia were selectively labeled with [3H]leucine or [35S]methionine in one compartment of a lucite chamber. Transport of TCA-precipitable material was monitored in the spinal roots and sciatic nerve kept in another compartment. Fastest transport rates were 75-90 mm/day at 18 decrees C. Ligation of the nerve 30 mm distal to the 8th ganglion at the beginning of the experiment resulted in accumulation of label during a 24 h period. This material was subjected to two-dimensional electrophoresis (pI 5-8; mol.wt. 10(4)-10(5) daltons) in 3 mm nerve segments. Autoradiographs or fluorographs from segments proximal to the ligature yielded a pattern of about 140 spots. Of these, at least 60 were considered to be independent protein species. Neither actin nor tubulin were present among these rapidly-transported, labeled proteins. No pattern was observed from segments distal to the ligature. Blocking protein synthesis with 18 micrometer anisomycin reduced the accumulation of label proximal to the ligature by 98%. Direct labeling of nerve segments produced patterns significantly different from the pattern of transported proteins.

Animals↗
PubMed1978

Changes in synthesis of specific proteins following axotomy: detection with two-dimensional gel electrophoresis.

Changes in protein synthesis during development and following axotomy were analyzed by two-dimensional gel electrophoresis. The two major postganglionic nerves emerging from the superior cervical sympathetic ganglia (SCSG) of adult rats were either cut or crushed unilaterally. At intervals ranging from 1 to 112 days after surgery both SCSG were removed and incubated for 1 hr in the presence of 14C-leucine. Proteins were extracted and subjected to two-dimensional electrophoretic separation and autoradiography. With this technique, proteins are separated on the basis of isoelectric point and molecular weight. Also, intact SCSG from 1, 2, 7, and 14 day old rats were labeled and analyzed. It was found that a minority of the separated proteins exhibited some detectable change in relative rate of synthesis following axotomy. Actin exhibited a slight (less than 20%) increase in relative synthesis rate while tubulin did not change significantly. There were small but significant differences in the protein patterns following nerve crush, as opposed to nerve cut. Comparison of protein synthesis patterns from developing rat SCSG with those from intact and from axotomized adult SCSG failed to demonstrate any marked similarity between the developmental and the axotomy patterns.

Actins↗
PubMed1978

Test of some aging hypotheses using two-dimensional protein mapping.

We have compared the proteins from superior cervical sympathetic ganglia (SCSG) in young and old male Fischer rats. We have searched for the kinds of changes in protein size or charge which are predicted by such cellular aging theories as the error catastrophe theory, the somatic mutation theory, the cross-linkage theory, translational aging, the deamidation theory, and the autoimmune theory. SCSG from young (2 months to 1 year) and old (21-24 months) rats were labeled in vitro for 1 h with 14C-leucine prior to protein extraction for analysis. Protein analysis was on high-resolution, two dimensional polyacrylamide gels using O'Farrell's technique, with some modifications. Proteins were separated according to isoelectric point in one dimension and molecular weight in the second dimension. The changes predicted by the above aging theories were detected neither in the staining pattern, showing protein amounts, nor in the labeling pattern on autoradiographs of dried gels, showing newly synthesized proteins. The results suggest the possibility that, at least for rat, some other mechanisms for aging are present.

Aging↗
PubMed1977

Simultaneous assay of the methylxanthine metabolites of caffeine in plasma by high performance liquid chromatography.

A fast and sensitive method by high performance liquid chromatography for the quantification of the methylxanthine metabolites of caffeine in human plasma is described. Plasma is extracted with 20% isopropanol in chloroform and the evaporated residue is redissolved and chromatographed on silica gel using dichloromethane containing 2.2% of a solution of methanol-ammonium formateformic acid (100:0.02:0.017). The method is suitable for metabolic and pharmacokinetic studies of all mono- and dimethylxanthines in plasma. The limits of quantification, with C.V. of 10% or less are 40 ng/ml for the dimethylxanthines, 100 ng/ml for 3- and 7-methylxanthine and 500 ng/ml for 1-methylxanthine.

Caffeine↗