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D L Wilson

Publications and source records attributed to D L Wilson.

At least 37 records · Page 2Linked to original sources

Perception of temporally filtered X-ray fluoroscopy images.

For noisy X-ray fluoroscopy image sequences we quantitatively evaluated image quality after digital temporal filtering to reduce noise. Using an experimental paradigm called a reference/test adaptive forced-choice method we compared detectability of stationary low-contrast disks in filtered and unfiltered, computer-generated image sequences. In the first experiment, a low-pass first-order recursive filter used in X-ray fluoroscopy was found to be much less effective at enhancing detectability than predicted from the reduction of display noise variance, a common measurement of filter effectiveness. Detectability was reasonably predicted by a nonprewhitening human-observer model (NPW-HVS) that included an independently determined human temporal-contrast-sensitivity function. In another experiment, designed to test models over a range of temporal frequencies, we used paired high-pass and low-pass temporal filters that both reduced noise variance by 25%. The high-pass filter was artificially applied to the noise only and greatly improved detectability, while the low-pass filter had little effect. The human-observer model quantitatively described the measurements, but classical prewhitening and nonprewhitening signal detectors did not. As compared to the nonprewhitening, spatio-temporal matched filter, human-observer efficiency was low and variable at 2.1%, 2.9%, and 0.06% for 60 frames of unfiltered low-pass and high-pass noise, respectively. As compared to this detector, humans were not very effective at combining information across frames. On the other hand, signal to noise ratios (SNR's) from the human-observer model were comparable to human performance, and efficiencies were reasonably constant at 40%, 52%, and 32%, respectively. We conclude that it is imperative to include human-observer models and experiments in the analysis of noise-reduction filtering of noisy image sequences, such as X-ray fluoroscopy.

Adult↗

Modeling and optimization of rotational C-arm stereoscopic X-ray angiography.

Stereoscopy can be an effective method for obtaining three-dimensional (3-D) spatial information from two-dimensional (2-D) projection X-ray images, without the need for tomographic reconstruction. This much-needed information is missed in many X-ray diagnostic and interventional procedures, such as the treatment of vascular aneurysms. Fast C-arm X-ray systems can obtain multiple angle sequences of stereoscopic image pairs from a single contrast injection and a single breath hold. To advance this solution, we developed a model of stereo angiography, performed perception experiments and related results to optimal acquisition. The model described horizontal disparity for the C-arm geometry that agreed very well with measurements from a geometric phantom. The perceptual accommodation-convergence conflict and geometry limited the effective stereoscopic field of view (SFOV). For a typical large image intensifier system, it was 28 cm x 31 cm at the center of rotation (COR). In the model, blurring from finite focal-spot size and C-arm motion reduced depth resolution on the digital display. Near the COR, the predicted depth resolution was 3-11 mm for a viewing angle of 7 degrees , which agreed favorably with results from recently published studies. The model also described how acquisition parameters affected spatial warping of curves of equal apparent depth. Pincushioning and the difference between the acquisition and display geometry were found to introduce additional distortions to stereo displays. Preference studies on X-ray angiograms indicated that the ideal viewing angle should be small (1-2 degrees), which agreed with some previously published work. Perceptual studies indicated that stereo angiograms should have high artery contrast and that digital processing to increase contrast improved stereopsis. Digital subtraction angiograms, with different motion errors between the left and right-eye views, gave artifacts that confused stereopsis. The addition of background to subtracted images reduced this effect and provided other features for improved depth perception. Using the modeling results and typical clinical angiography requirements, we recommend acquisition protocols and engineering specifications that are achievable on current high-end systems.

Angiography, Digital Subtraction↗

An adaptive segmentation algorithm for time-of-flight MRA data.

A three-dimensional (3-D) representation of cerebral vessel morphology is essential for neuroradiologists treating cerebral aneurysms. However, current imaging techniques cannot provide such a representation. Slices of MR angiography (MRA) data can only give two-dimensional (2-D) descriptions and ambiguities of aneurysm position and size arising in X-ray projection images can often be intractable. To overcome these problems, we have established a new automatic statistically based algorithm for extracting the 3-D vessel information from time-of-flight (TOF) MRA data. We introduce distributions for the data, motivated by a physical model of blood flow, that are used in a modified version of the expectation maximization (EM) algorithm. The estimated model parameters are then used to classify statistically the voxels into vessel or other brain tissue classes. The algorithm is adaptive because the model fitting is performed recursively so that classifications are made on local subvolumes of data. We present results from applying our algorithm to several real data sets that contain both artery and aneurysm structures of various sizes.

Algorithms↗

Determining x-ray projections for coil treatments of intracranial aneurysms.

The endovascular coil embolization of intracranial saccular aneurysms requires a set of specific X-ray images with which to view the aneurysm during coiling. These two-dimensional (2-D) images, known as working projections, should be optimal for measuring the aneurysm sac diameter, inserting the first coil, and checking coil overhang into the surrounding vessels. At present the gantry tilt that produces these images is found by the radiologist by trial and error. In this paper, we present a method for automatically finding the angles that will produce the desired X-ray projections. Our method consists of four steps: 1) finding the location and orientation of the aneurysm neck; (2) labeling the aneurysm sac; 3) determining the optimal tilts for viewing the aneurysm during coiling; and 4) adjusting the optimal tilts for change in the patient orientation between pre-Guglielmi detachable coil (GDC) scanning and the coiling treatment. We discuss these steps and present results of the algorithm applied to pathological examples in the form of simulated X-ray images. A final discussion is given for one example where our results have been applied in a clinical situation.

Algorithms↗

Role of phase information and eye pursuit in the detection of moving objects in noise.

As part of an ongoing study that uses objective image quality measures to optimize medical imaging x-ray fluoroscopy, we investigated two basic features of the detection of moving cylinders that mimic arteries, catheters, and guide wires. First, we compared detection with and without a phase cue consisting of a nearby alternating light and dark square. Depending on object size and velocity, phase cuing improved detection from 1% to 15% and gave an average of 6%, an effect much smaller than the 38% predicted from a Monte Carlo simulation of the ideal observer. Evidently, humans were limited in their ability to incorporate knowledge of the phase cue. Second, we evaluated the effect of eye pursuit of a fixation point that moved with the target. In general, motion at the highest velocity degraded (74%) and enhanced (68%) detection of small and large objects, respectively. With eye pursuit, both effects were substantially reduced in a manner consistent with a reduced retinal velocity. Our data compared favorably with a human observer model that included a spatiotemporal contrast sensitivity response and smooth-pursuit eye movements with a gain of 0.8. These mechanisms of perception are thought to be present in coronary artery x-ray fluoroscopy imaging, where phase information is available from the moving heart and where motion markers are available from x-ray opaque markers incorporated in thin catheters and guide wires.

Artifacts↗

Detection improvement in spatially filtered x-ray fluoroscopy image sequences.

The effect of spatial noise-reduction filtering on human observer detection of stationary cylinders mimicking arteries, catheters, and guide wires in x-ray fluoroscopy was investigated in both single image frames and image sequences. Ideal edge-preserving spatial filtering was simulated by filtering of the noise before addition of the target cylinder. This allowed us to separate the effect of edge blurring from those of noise reduction and spatial noise correlation. We used three different center-weighted averagers that reduced pixel noise variance by factors of 0.75, 0.50, and 0.25. As compared with no filtering, the effect of filtering on detection in single images was statistically insignificant. This indicated an adverse effect of spatial noise correlation on detection that countered the effect of noise reduction. By comparison, spatial filtering significantly improved detection in image sequences and yielded potential x-ray dose savings of 26-34%. Comparison of results with two observer models suggested that human observers have an improved detection efficiency in spatially filtered image sequences as compared with white-noise sequences. Pixel noise reduction, a measure commonly used to assess filter performance, overestimated the effect of filtering on detection and was not a good indicator of image quality. We conclude that edge-preserving spatial filtering is more effective in sequences than in single images and that such filtering can be used to improve image quality in noisy image sequences such as x-ray fluoroscopy.

Contrast Sensitivity↗

Brachytherapy in early prostate cancer--early experience.

Use of brachytherapy with radioactive seeds in the management of early prostate cancer is commonly used in the United States. The early experience has been reported from the prostate treatment centers in Seattle for the last 10 years. In this manuscript we are reporting our early experience of 150 radioactive seed implantations in early stage prostate cancer using either Iodine 125 or Palladium 103 seeds. The average age of the patient is 66 years and the median Gleason score is 5.4 with a median PSA of 6. A brief description of the evolution of the treatment of prostate cancer as well as the preparation for the seed implantation using the volume study with ultrasound of the prostate, pubic arch study using CT scan of the pelvis and the complete planning using the treatment planning computers are discussed. We also have described the current technique which is used in our experience based on the Seattle guidelines. We plan a follow-up report with the results of the studies with longer follow-up.

Aged↗

C57BL/6 mice fed high fat diets as models for diabetes-accelerated atherosclerosis.

Non-insulin-dependent diabetes mellitus (NIDDM) is a major risk factor for the development of atherosclerosis in humans. The development of an animal model that displays accelerated atherosclerosis associated with NIDDM will aid in elucidating the mechanisms that associate these disorders. C57BL/6 mice may provide such a model system. This strain becomes obese, hyperglycemic and insulin resistant when fed a high fat diet (diabetogenic diet) and is susceptible to atherosclerotic lesion development when fed a separate high fat diet containing cholesterol and bile acids (atherogenic diet). This report tests whether a diet commonly used to induce atherosclerosis also provokes a diabetic phenotype and whether a diet used to induce diabetes provokes the development of aortic fatty streak lesions. Mice of strains C57BL/6, C3H/He, BALB/c and seven recombinant inbred (RI) strains were fed an atherogenic diet for 14 weeks and glucose parameters were measured. No correlation was observed between atherosclerosis susceptibility and fasting insulin or glucose levels, or glucose clearance following short-term insulin or glucose treatment. Analysis of the RI strains suggested that multiple genes control these glucose metabolic parameters. Feeding the diabetogenic diet for 14 weeks to C57BL/6 mice induced obesity and diabetes and 2-fold increases in plasma lipoprotein concentrations. Also, small aortic sinus lipid deposits were observed in 40% of the mice. Thus, analysis of the diabetogenic diet fed C57BL/6 mouse may provide an important tool for further studies of diabetes accelerated vascular disease.

Animals↗

Effects of motion blurring in x-ray fluoroscopy.

In conventional fluoroscopy, continuous x-ray exposure blurs moving objects, while in pulsed fluoroscopy, short duration x-ray pulses acquire images without motion blur. Many perception experiments on noisy image sequences are consistent with low-pass temporal filtering by the human visual system, and this is anticipated to cause visual system "blurring" of moving objects. With moving cylinders in spatially white noise, we simulated 30 acq/s (acquisitions per second), continuous fluoroscopy having both x-ray and visual system motion blur. We also simulated pulsed fluoroscopy at 30 acq/s (pulsed-30) having visual system but not x-ray system motion blur. For both continuous and pulsed-30 acquisitions, with increasing velocity, detectability of small cylinders decreased by as much as approximately 50%, while detectability of large cylinders increased and then decreased. Detectability of pulsed-30 was only slightly higher than continuous, indicating that visual system motion blurring dominated x-ray system blurring. For the case of stationary objects, blurring greatly reduced detectability, indicating that last-image-hold of moving objects deteriorates with continuous acquisitions. With no free parameters, a human observer model with an independently measured spatio-temporal contrast sensitivity function accurately described all effects.

Contrast Sensitivity↗

Detection of moving objects in pulsed-x-ray fluoroscopy.

We investigated the detectability of moving, low-contrast objects in white-noise image sequences. The computer-generated, cylindrical phantoms mimicked arteries, catheters, and guide wires in medical, x-ray fluoroscopy image sequences at 16 acquisitions/s (pulsed-16) or 32 acquisitions/s (pulsed-32). We measured detectability by using a reference-test, adaptive forced-choice method whereby reference and test presentations were alternated during an experimental session to minimize effects of subject attention and accuracy criteria. In the case of the largest cylinder (diameter 0.48 deg), the highest speed (5.86 deg/s) increased absolute detectability by approximately 42% compared with that in the stationary case. With the smallest cylinder (diameter 0.023 deg), this motion decreased detectability by approximately 51%. The dose savings of pulsed-16 was approximately 18% of that for pulsed-32, with relatively little effect of velocity or object size. In general, subjects took slightly longer to respond in the case of low-acquisition fluoroscopy. Detectability data were modeled with a nonprewhitening matched filter that included a physiological, spatiotemporal contrast sensitivity function and a suboptimal, spatiotemporal signal template with time-limited memory.

Fluoroscopy↗

Diminished reproduction, failure to thrive, and altered immunologic function in a colony of T-cell receptor transgenic mice: possible role of Citrobacter rodentium.

Citrobacter rodentium from an undetermined source was detected in a breeding colony of T-cell receptor transgenic mice housed in a conventional mouse facility in which murine hepatitis virus had been endemic and Helicobacter spp. had been detected. Citrobacter rodentium, isolated from blood, spleen, and colon, correlated with a significant increase in mortality and morbidity in this breeding colony. Transgenic mice of all ages were affected by chronic debilitation, loss in reproductive efficiency, rectal prolapse, and acute death, resulting in the near loss of these noncommercially available strains. Several alterations in immunologic parameters were observed, including outgrowth of an unusual population of cells in the spleen and blood, reduction in ascites production, loss of the capacity of peritoneal exudate cells to serve as feeders for the cloning of long-term T-cell lines, and inhibition of antigen-specific cytotoxic T-cell activity. These altered immune functions also were apparent in commercially-derived nontransgenic mice cohoused with the infected colony and in overtly healthy transgenic and nontransgenic littermates. Citrobacter rodentium and murine hepatitis virus were eliminated ultimately on rederivation of the affected strains by embryo transfer. However, the rapid decrease in the health of the colony necessitated more immediate action. To reduce mortality and allow breeding to continue during rederivation of the transgenic lines, animals were treated with enrofloxacin and moved to a barrier facility. Antibiotic therapy significantly reduced morbidity and mortality, markedly increased litter size and frequency, and resulted in the normalization of many of the immunologic assays. The involvement of C. rodentium in altering viability of the colony and perturbing immunologic assays is suggested by correlation of the onset of the syndrome with the appearance of Citrobacter sp. and its resolution with the elimination of Citrobacter sp. from the colony. Whether infection with Citrobacter alone is causative or whether superinfection of murine hepatitis virus- and Helicobacter-infected mice is required remains to be determined.

Animals↗

The percentage of consonants correct (PCC) metric: extensions and reliability data.

Research in normal and disordered phonology requires measures of speech production that are biolinguistically appropriate and psychometrically robust. Their conceptual and numeric properties must be well characterized, particularly because speech measures are increasingly appearing in large-scale epidemiologic, genetic, and other descriptive-explanatory database studies. This work provides a rationale for extensions to an articulation competence metric titled the Percentage of Consonants Correct [PCC; Shriberg & Kwiatkowski, 1982; Shriberg, Kwiatkowski, Best, Hengst, & Terselic-Weber, 1986], which is computed from a 5- to 10-minute conversational speech sample. Reliability and standard error of measurement estimates are provided for 9 of a set of 10 speech metric including the PCC. Discussion includes rationale for selecting one or more of the 10 metrics for specific clinical and research needs.

Adolescent↗

The speech disorders classification system (SDCS): extensions and lifespan reference data.

A companion paper includes rationale for the use of 10 metrics of articulation competence in conversational speech (Shriberg, Austin Lewis, McSweeny, & Wilson, 1997). The present paper reports lifespan reference data for these measures using records from a total of 836 3- to 40(+)-year-old speakers with normal and disordered speech. The reference data are subdivided by diagnostic classification based on extensions to an instrument titled the Speech Disorders Classification System (SDCS; Shriberg, 1993). Appendices provide procedural information on the SDCS and statistical rationale for the reference data.

Adolescent↗

Isolation of a cDNA encoding a Kex2-like endoprotease with homology to furin from the nematode Caenorhabditis elegans.

A cDNA was isolated from the nematode Caenorhabditis elegans that encodes an endoprotease which is a member of the Kex2 family of serine endoproteases. Degenerate oligonucleotide primers were designed based on conserved regions within the active sites of known Kex2-like endoproteases, and were used for reverse transcription-polymerase chain reaction (RT-PCR) of poly(A)+RNA isolated from C. elegans. A PCR product was isolated that had homology to the active sites of known furin endoproteases, and was used as a probe to screen a C. elegans cDNA library. A Kex2-like endoprotease (CelfurPC) which encoded a 692-amino-acid pre-proendoprotease, was identified. The deduced amino acid sequence for the catalytic domain of CelfurPC is homologous to the known Kex2-like endoproteases, with strongest structural homology to the furin/PACE4 family. However, all furins and PACE4 proteins contain a characteristic cysteine-rich domain, and all furins contain a transmembrane domain, neither of which is present in the CelfurPC protein. CelfurPC may thus represent a new class of Kex2-like endoprotease.

Amino Acid Sequence↗

Three dimensional structure of human fibrinogen under aqueous conditions visualized by atomic force microscopy.

Fibrinogen plays a central role in surface-induced thrombosis. However, the interactions of fibrinogen with different substrata remain poorly understood because of the difficulties involved in imaging globular proteins under aqueous conditions. We present detailed three dimensional molecular scale images of fibrinogen molecules on a hydrophobic surface under aqueous conditions obtained by atomic force microscopy. Hydrated fibrinogen monomers are visualized as overlapping ellipsoids; dimers and trimers have linear conformations predominantly, and increased affinity for the hydrophobic surface compared with monomeric fibrinogen. The results demonstrate the importance of hydration on protein structure and properties that affect surface-dependent interactions.

Fibrinogen↗

Shear-dependent changes in the three-dimensional structure of human von Willebrand factor.

The three-dimensional tertiary structure of human von Willebrand Factor (vWF) on a hydrophobic surface under aqueous conditions and different shear stress regimes was studied by atomic force microscopy (AFM). vWF was imaged by AFM at molecular level resolution under negligible shear stress, under a local applied shear force (7.4 to 19 nN) using the AFM probe in contact mode scanning, and after subjecting vWF to a range of shear stress (0 to 42.4 dyn/cm2) using a rotating disk system. The results demonstrate that vWF undergoes a shear stress-induced conformational transition from a globular state to an extended chain conformation with exposure of intra-molecular globular domains at a critical shear stress of 35 +/- 3.5 dyn/cm2. The globular vWF conformation (149 nm by 77 nm and height 3.8 nm) is representative of native vWF after simple diffusion to the hydrophobic surface, followed by adhesion and some spreading. In a shear stress field above the critical value, protein unfolding occurs and vWF is observed in extended chain conformations oriented in the direction of the shear stress field with molecular lengths ranging from 146 to 774 nm and 3.4 nm mean height. The shear stress-induced structural changes to vWF suggest a close conformation-function relationship in vWF properties for thrombogenesis in regions of high shear stress.

Chemical Phenomena↗

Increased atherosclerosis in streptozotocin-induced diabetic mice.

Premature and extensive atheroscleroses involving renal, peripheral, and cardiovascular sites remain major complications of diabetes mellitus. Controversy exists as to the contribution of hyperglycemia versus elevated local or systemic concentrations of insulin to atherosclerosis risk. In this report, we developed the first murine model susceptible to both atherosclerosis and diabetes to determine which diabetogenic factors contribute to vascular disease. C57BL/6 and BALB/c mice were treated with multiple low-dose streptozotocin (STZ) or control citrate buffer and fed rodent chow or an atherogenic-promoting (Ath) diet for 12-20 wk. STZ treatment resulted in sustained hyperglycemia (250-420 mg/dl) and a modest reduction in plasma insulin levels for both strains regardless of diet. Citrate-treated C57BL/6 mice fed the Ath diet showed extensive oil red O-staining fatty streak aortic sinus lesions (20,537+/-2,957 micron2), the size of which did not differ for Ath-fed mice treated with STZ (16,836+/-2,136 micron2). In contrast, hyperglycemic BALB/c mice fed the Ath diet showed a 17-fold increase in atherosclerotic lesion area (7,922+/-2,096 micron2) as compared with citrate-treated mice fed the Ath diet (467+/-318 micron2). Correlations between lesion size and plasma glucose levels were significant for BALB/c (r = 0.741, P < 0.009), but not C57BL/6 (r = 0.314, P<0.3) mice. Lesion size correlated significantly with plasma cholesterol for C57BL/6 (r = 0.612, P<0.03) but not BALB/c (r = 0.630, P<0.1) mice. Immunohistochemistry showed that aortic sinus lesions from both strains contained macrophages, but smooth muscle cells were clearly present in lesions of BALB/c mice. In summary, we present the first small animal model showing accelerated atherosclerosis in response to hyperglycemia. Fatty streaks resembled those of human type II lesions in that both macrophages and smooth muscle cells were evident. In addition, our results support the concept that hyperglycemia as opposed to hyperinsulinemia contributes heavily to risk of atherosclerosis.

Animals↗

Eye movement desensitization and reprocessing: effectiveness and autonomic correlates.

Eighteen subjects distressed by memories of a specific traumatic event were randomly assigned to a single session of one of three conditions: Eye Movement Desensitization and Reprocessing (EMDR), a Time Interval Condition (TIC), or Tapping Alternate Phalanges (TAP). All subjects treated in the EMDR group showed desensitization as monitored by SUDs, which correlated with the physiological data and cessation of pronounced symptomatology. Only one subject in a control group showed desensitization. Compared to TIC and TAP, autonomic measures showed distinct changes during EMDR: (1) respiration synchronized with the rhythm of the eye movements in a shallow, regular pattern; (2) heart rate slowed significantly overall; (3) systolic blood pressure increased during early sets, invariably declined during abreactions, and decreased overall; (4) finger tip skin temperature consistently increased; and (5) the galvanic skin response consistently decreased in a clear "relaxation response". This relaxing effect of the eye movements suggests that at least one of the mechanisms operating during EMDR is desensitization by reciprocal inhibition, by pairing emotional distress with an unlearned or "compelled" relaxation response.

Adult↗