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D L Rhoden

Publications and source records attributed to D L Rhoden.

30 records · Page 2Linked to original sources
PubMed1975

Evaluation of the pathotec Rapid I-D system for identification of Enterobacteriaceae.

The PathoTec Rapid I-D System for identifying Enterobacteriaceae was evaluated with 471 cultures. In 4,910 individual test comparisons, 95.5% of the results agreed, with results of only two test strips, those for esculin hydrolysis and urease production, agreeing with conventional tests in less than 94% of the trials. The PathoTec system exhibited 94.3% accuracy in identifying these cultures in a double-blind study with conventional media and procedures as the alternate system. Two newly developed test strips, for 0-nitrophenyl-beta-D-galactopyranoside and ornithine decarboxylase, were found to be highly reliable.

Classification↗
PubMed1974

r-b Expanders: their use in identifying routinely and unusually reacting members of Enterobacteriaceae.

This cooperative study between a large clinical laboratory and a reference laboratory evaluated the performance of the expanded r/b system for identifying Enterobacteriaceae. The 2,200 cultures isolated in the normal hospital routine presented no problem of identification to the r/b system. About 250 "atypical" cultures which were exchanged between the collaborating laboratories were also identified accurately. The expanded r/b system was found to perform as well as most biochemical-physiological identification systems, and when used appropriately was highly satisfactory as a system for identification of Enterobacteriaceae.

Arabinose↗
PubMed1973

Auxotab--a device for identifying enteric bacteria.

A multitest system called the Auxotab that uses ten dehydrated reagents on a paper card has been evaluated with 417 known stock cultures of Enterobacteriaceae. In double-blind studies with the Auxotab, 87% of the strains tested were correctly identified. Results of this study indicate that there is a need for modification of the product in regard to ease of handling, time required for use, and accuracy of identification of enteric bacteria.

Bacteriological Techniques↗
PubMed1973

Evaluation of the redesigned enterotube--a system for the identification of Enterobacteriaceae.

The redesigned Enterotube has been evaluated with 414 unknown Enterobacteriaceae cultures from the stock culture collection of the Center for Disease Control. When the Enterotube was used as recommended by the manufacturer, an average of 96.4% of these cultures were correctly identified. Only two groups (Salmonella and Edwardsiella) were identified with less than 90% accuracy (89.2 and 87.5%, respectively). The Enterotube now provides a convenient, rapid, and accurate test system for the identification of typically reacting enteric bacteria.

Bacteriological Techniques↗
PubMed1971

R-B enteric differential system for identification of Enterobacteriaceae.

The R/B Enteric Differential System for identifying enteric bacteria has been evaluated with 451 "unknown" cultures from the stock culture collection of the Center for Disease Control. An average of 89.6% of these cultures were correctly identified by the R/B system, when used as recommended by the manufacturer but without the assistance of serology. This percentage ranged, however, from 47% for Klebsiella to 100% for Serratia and Providencia. Of 11 groups or genera of Enterobacteriaceae tested, only three (Enterobacter, Serratia, and Providencia) were identified with 95% or better accuracy. Four groups (Arizona, Citrobacter, Escherichia, and Salmonella) attained 90 to 95% accuracy of identification, and three groups (Edwardsiella, Proteus, and Shigella) scored between 85 and 90% accuracy. We recommend the R/B system as a screening device which is reasonably successful in grouping bacteria but not as a substitute for more exacting conventional procedures.

Bacteriological Techniques↗
PubMed1969

Improved medium for detecting deoxyribonuclease-producing bacteria.

Incorporation of methyl green dye into an agar medium containing deoxyribo-nucleic acid results in an improved medium for detecting deoxyribonuclease-producing bacteria. Use of the dye makes it unnecessary to use acid to demonstrate deoxyribonuclease activity, thus allowing subculture or reincubation of colonies. The improved medium is highly sensitive and can be used for primary isolation of Staphylococcus aureus or group A Streptococcus pyogenes.

Bacteria↗