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Biomedical subjects

D Katz

Publications and source records attributed to D Katz.

At least 217 records · Page 12Linked to original sources
PubMed1974

Fusion and compatibility of camphor and octane plasmids in Pseudomonas.

The octane (OCT) plasmid in Pseudomonas putida derived from the omega-hydroxylase-carrying strain of Coon and coworkers is transferable to the camphor (CAM) plasmid-bearing strain by conjugation or by transduction. While the majority of the Cam (+)Oct(+) exconjugants segregate Cam(+) or Oct(+) cells, exconjugants with stable Cam (+)Oct(+) phenotype (CAM-OCT) can be detected at a low frequency. The transductants are all of the CAM-OCT phenotype. In the stable Cam (+)Oct(+) strains, the OCT plasmid resembles the CAM plasmid with respect to curing by mitomycin C, transfer in conjugation, and reaction to ts (temperature-sensitive) mutation specifically affecting CAM plasmid replication. Therefore, it is suggested that certain regions of homology exist between the CAM and OCT plasmids that enable them to recombine to form a single plasmid, and to overcome the incompatibility barrier that prevents their coexisting.

Alkanes↗
PubMed1973

Subapical wall synthesis and wall thickening induced by cycloheximide in hyphae of Aspergillus nidulans.

Hyphae of Aspergillus nidulans continued to synthesize all the major polysaccharide components of the cell wall when cycloheximide was added to cultures. In the presence of cycloheximide, hyphae did not elongate, but electron microscopy showed that the walls became thicker around the cell. The conclusion that cycloheximide changed wall synthesis from extension at the apex to subapical thickening was supported by grain distributions on radioautograms of mutant hyphae labeled with galactose. These findings are discussed in relation to the control of hyphal wall synthesis and are compared with the effects of protein synthesis inhibitors on wall formation in gram-positive bacteria.

Aspergillus↗
PubMed1972

Model for branch initiation in Aspergillus nidulans based on measurements of growth parameters.

The rate of hyphal elongation and the number of branches per hypha were measured on short sporelings of Aspergillus nidulans growing at different rates. The rate of elongation was proportional to total length in unbranched and branched hyphae. At each growth rate, the number of branches per hypha increased with increasing length and gave approximately straight-line graphs when plotted against length. The average number of branches per unit of hyphal length was quite different for the various growth rates and increased in direct proportion to the growth rate. The results are interpreted to mean that (i) growing tips have a maximum rate at which they can elongate and which is reached at hyphal lengths characteristic of the particular growth rate and (ii) a new branch is formed when the capacity of the hypha to elongate exceeds that of the existing tips.

Acetates↗
PubMed1971

Hyphal wall synthesis in Aspergillus nidulans: effect of protein synthesis inhibition and osmotic shock on chitin insertion and morphogenesis.

Pulse-labeling with N-[acetyl-(3)H] glucosamine and radioautography were used to follow the sites of chitin incorporation in hyphae of an Aspergillus nidulans mutant blocked in amino sugar synthesis. Growing hyphae incorporated N-acetylglucosamine almost exclusively at the tip. Cycloheximide addition greatly increased the label in subapical regions of the hyphae and reduced that at the tip. This effect of cycloheximide was immediate, could be reversed by removing the inhibitor, and did not appear to be due to chitin turnover. A similar change from apical to subapical N-acetylglucosamine incorporation occurred after hyphae were subjected to an osmotic shock which did not inhibit protein synthesis. The two treatments induced morphogenetic changes in the hyphae which produced abnormally large numbers of branches and septa.

Acetates↗