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Biomedical subjects

D Katenkamp

Publications and source records attributed to D Katenkamp.

At least 109 records · Page 6Linked to original sources
PubMed1989

Lectin histochemistry of human testicular germ cell tumors.

The lectin binding pattern (WGA, UEA-I, PNA, PSA, Con A, RCA and LCA) of 28 human testicular germ cell tumors (pure or combination tumors) was investigated. Lectin binding sites could be demonstrated in all germ cell tumor types. In classic and spermatocytic seminomas as well as seminomas with high mitotic index an equal distribution of lectin binding sites was observed. In embryonal carcinomas the lectin binding of UEA-I, PNA, WGA, LCA and RCA correlated to histological differentiation. A polarized staining of WGA, PNA, RCA and UEA-I, typical for embryonal carcinomas, yolk sac tumors and teratomas, was never seen in seminomatous tumors and may be of importance in differential diagnosis. By means of Con A decoration it was possible to distinguish cytotrophoblastic and syncytiotrophoblastic differentiation. Aspects of lectin histochemistry in tumor biology in general and in differential diagnosis of germ cell tumors in particular are discussed.

Choriocarcinoma↗
PubMed1989

[Thoracic actinomycosis].

Actinomycosis is an infectious disease distributed all over the world. Males are affected three times more than females; the incidence of the disease is higher in adults than in children. The main causative agent is Actinomyces israelii, which can be found in the oropharynx of healthy persons. Therefore actinomycosis represents always an endogenous infection, often in connection with other bacteriae. The thoracic manifestation amounts to approximately 15 percent of all cases. The main clinical symptoms are pain, fever, cough, fatigue, and weight loss. The blood picture shows an increased number of white blood cells with a left shift in the differential white blood cell count. Furthermore, there is a strongly raised erythrocyte sedimentation rate. Radiographs demonstrate no typical patterns of pulmonary abnormalities. For the diagnosis there is a need of anaerobic cultures, in second line histological examination of tissue specimens. Differential diagnosis of actinomycosis includes chronic pneumonias caused by other rare germs and neoplasms. After early onset antibiotic long term treatment a good prognosis may be expected. Penicillin represents the antibiotic of first choice.

Actinomycosis↗
PubMed1989

[The prognostic significance of the ultrastructural characteristics of human rectal cancer].

A retrospective study of the ultrastructure of rectal tumors in patients with favorable and unfavorable prognosis was undertaken. It established favorable postoperative course and prognosis in tumors containing more than half undifferentiated cells. Conversely, tumors in which malignant cells with electron microscopy-identifiable signs of differentiation (retaining features of normal cells) prevailed and those containing equal fractions of well- and poorly differentiated cells involve early development of metastasis and sometimes recurrences.

Adenocarcinoma↗
PubMed1988

In adequate tumor surgery of chemically induced soft tissue sarcomas--an experimental approach for induction of metastasis formation?

In preceding experiments with nude mice bearing xenotransplanted soft tissue sarcomas after inoculation of cultured sarcoma cells we attained a high percentage of metastatic dissemination by repeated inadequate tumor surgery. In the study presented here we used methylcholanthrene induced sarcomas (rhabdomyosarcomas and undifferentiated sarcomas in part showing the picture of storiform/pleomorphic malignant fibrous histiocytomas) which were produced in NMRI mice and examined (1) whether or not metastasis formation could be triggered or enhanced by repeated inadequate tumor surgery in these animals, and (2) the efficacy of the same surgical procedure following xenotransplantation of the tumors into nude mice. We did not reach an increasing frequency with both experimental arrangements. After discussing several factors which could be in general responsible for inducing metastasis formation and after comparing the results of our several experiments it is suggested that the methylcholanthrene induced soft tissue tumors may not contain cell clones able to metastasize.

Animals↗
PubMed1988

Cellular heterogeneity. Explanation for changing of tumor phenotype and biologic behavior in soft tissue sarcomas.

Many human soft tissue sarcomas are known to show variable structures in a given tumor and to be able of changing the histological feature during the course of the disease. These facts prompted an experimental study in order to ascertain if by use of long-term cultures of an established rat fibrosarcoma cell line (RFS) in respective inoculation tumors a histological picture different from the original fibrosarcoma could be produced. It was found that inoculation tumors generated by later subcultures partly corresponded to tumors with features of malignant histiocytomas and malignant fibrous histiocytomas. In a further experiment we attempted to initiate metastatic dissemination of a primarily non-metastasizing xenotransplanted RFS sarcoma in nude mice. By repeated diminution operations comparable to inadequate tumor surgery in human metastasis formation in lung parenchyme was attained. Taking into account cellular heterogeneity as basically responsible for the phenomena observed, different causal aspects concerning the experimental results are discussed. We deduce from our findings that the concept of histogenetic classification of soft tissue sarcomas should be replaced by an interpretation according to the actual differentiation. This also applies to malignant fibrous histiocytomas which are at least in part mesenchymal tumors and not descendants of mononuclear phagocytic cells and may originate by a dedifferentiation process of otherwise defined sarcoma types.

Animals↗
PubMed1988

Intermediate filament typing in tumour diagnostics--an aid in histogenetic classification?

It is commonly accepted, that a histogenetic correlation can be established between tumour and mother tissue by means of intermediate filament typing. Recent results concerning the co-expression of different intermediate filaments within one cell, the occurrence of epithelial-specific intermediate filaments in soft tissue tumours, and the modulation of intermediate filament type expression caused by some bioactive substances and viral infections cast doubts on the general acceptance of the histogenetic classification of human tumours by intermediate filament typing. Its characterization determines only the actual morphological differentiation of the neoplastic cells and represents a valuable tool for the diagnostic assessment of the tumour under the viewpoint of the functional differentiation of its cells. A doubtless conclusion concerning the mother tissue of the neoplasia (histogenetic origin) is therefore not possible.

Animals↗
PubMed1988

[Fibroma of the tendon sheath. Diagnosis and differential diagnosis].

Ten cases of fibroma of the tendon sheath were examined by means of histological, topochemical, and immunohistochemical methods. Investigations were aimed at re-evaluation of histological variability and definition of diagnostic criteria. Microscopic observations suggested the following features to be typical of fibroma of the tendon sheath: Primitive mesenchymal or fibroblastic cells without further differentiation, slit- and cleft-like spaces with lining cells decorated by endothelial markers, and collagenous, cartilaginous or myxoid basic substance at least in areas rich with sulphated glycosaminoglycans. Differential diagnostic distinction of tendon sheath fibroma from myxoid cartilage tumors, fasciitis nodularis, and myxoid fibromatosis as well as from fibrohistiocytic lesions and giant cell tumors of tendon sheath is discussed with reference to the above definition.

Adult↗
PubMed1988

[Angiomyoma. A pathologo-anatomic analysis of 229 cases].

An analysis was made of 229 cases of angiomyoma retrieved from the authors' own files. Studies were aimed at histological features, morphological variability, tumour localisation as well as at distribution by sexes and age groups. The angiomyoma cases were subdivided by histological features into solid forms (18 per cent), venous forms (38 per cent), combinations (43 per cent), and cavernous variants (two cases only). That subdivision was based on variable relationships between smooth muscles, on the one hand, and vascular cavities of different shapes, on the other. Angiomyoma was found to occur in high incidence in the fourth to sixth decennium of age, with clearly recordable predilection for females, and localized in 40 per cent of all cases in the region of the lower leg. Noteworthy peculiarities included predominance of male patients with combined or mixed angiomyoma with cavernous components or with solely cavernous forms and preference of the venous subtype for the head-neck region. The morphological structure of angiomyoma is discussed in some detail and is interpreted as a correlate of an organoid mixed tumour with muscular and vascular components. It is against this background that the term of "angiomyoma" is definitely preferred to "vascular leiomyoma".

Adolescent↗
PubMed1988

[Endometrial stromal sarcoma of vaginal and para-vaginal localization. Morphology, diagnosis and differential diagnosis].

Endometrial stromal sarcomas constitute a subgroup of malignant mesenchymal uterine neoplasms and comprise biologically somewhat different variants. Their occurrence in extrauterine tissues is possible but extremely rare and therefore they are quite often mistaken for other tumours. Reported in this paper is a stromal sarcoma in vaginal and paravaginal localization (64-years old woman). It was the purpose of our study to present the histological structure in comparison to uterine neoplasms and to discuss differential diagnosis. It could be demonstrated that histological features of the actual case corresponded precisely to the respective uterine tumours originating within the myometrium. In extrauterine position, haemangiopericytomas, leiomyosarcoma, malignant schwannomas, fibrosarcomas and synovial sarcomas as well as Müllerian adenosarcomas and carcinosarcomas should be taken into account. Important histological features facilitating distinction of endometrial stromal sarcomas from the aforementioned malignant tumours are discussed, and some comments are added with regard to histogenetical interpretation.

Diagnosis, Differential↗
PubMed1988

[Esthesioblastoma].

Histological appearance of esthesioblastomas shows wide-range variations contributing to difficulties in diagnosis. Five cases of the tumor analysed by the authors provided evidence on ultrastructural signs of the tumor cells neuroblasts-oriented differentiation, permitting ultrastructural differential diagnosis of esthesioblastoma with tumors of another histogenesis.

Adult↗
PubMed1987

[Disseminated neuroendocrine cancers of the skin--a cutaneous merkeliomatosis. Report of 2 cases].

Two nearly identical cases with about 100 multicentric disseminated neuroendocrine carcinomas of the skin are presented. In the Merkel cell tumors a for epithelial cells specific antigen (MAM-6) was found by immunohistochemical methods. Only in one patient generalized metastases arose after a four year course of the disease. The other patient committed suicide after a three year course of the disease. At autopsy no metastases were seen. We assume these diseases to be a systemic proliferation of malignant neuroendocrine differentiated epithelial cells, for which the term "cutaneous Merkeliomatosis" is proposed.

Aged↗
PubMed1987

Results of systemic chemotherapy of advanced bladder carcinomas (phase II study).

At the time of operation advanced carcinomas of the urinary bladder often have developed subclinical metastases. In 20 patients with metastases, inoperable carcinoma of the bladder responded to chemotherapy with a combination of adriamycin and 5-FU. 55% of the patients experienced either a complete or incomplete remission, which lasted long in some cases. In the future, suitable combinations of chemotherapeutics could participate in improving the prognosis of advanced carcinoma of the urinary bladder.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

Experimentally induced metastases of malignant fibrous histiocytomas xenotransplanted into nude mice from an established sarcoma cell line (RFS).

An experimental approach for inducing metastases from xenotransplanted malignant fibrous histiocytomas in nude mice is presented. Malignant fibrous histiocytomas were generated by inoculation of an established cell line (RFS) in the back of 16 nude mice. 7 nude mice were subjected to diminution operations carried out twice and three times, respectively, 6 out of these animals developed metastases, whereas no metastases were found in the inoperated control group (9 mice). The metastasis formation is explained by sarcoma heterogeneity, some factors which might contribute to the phenomenon of tumor dissemination are discussed.

Animals↗
PubMed1987

Adenomatoid tumors--mesotheliomas or not? A histochemical, immunohistochemical and light and electron microscopic (TEM/SEM) study.

A mesothelial, endothelial and epithelial differentiation of the adenomatoid tumors is discussed in the literature. Aimed at this problem the cellular nature of 12 adenomatoid tumors was investigated by means of histochemical and immunohistochemical methods at light and electron microscopic level. In all these neoplasias prekeratin was demonstrated while factor VIII-associated antigen and myoglobin were lacking within the tumor cells. The ultrastructural picture of the tumor cells was similar to that of mesothelial cells; abundant intermediate filaments of the keratin type could be decorated by means of the protein A-gold technique in them. Furthermore hyaluronidase sensitive glycosaminoglycans but no sulfated and neutral mucoproteins were found. The results suggest a mesothelial nature of the tumor cells of adenomatoid tumors.

Antigens↗
PubMed1987

[Results of treatment following stage-dependent therapy of non-seminomatous testicular tumors].

The results of the treatment of 194 patients with non-seminomas were analyzed and statistically registered. The therapeutic approach is described and the cumulative probability of survival is compared according to the stages. For the total number of these tumours a cumulative 5-year-survival probability of 68.6% was calculated. In detail it reaches from 100% in clinical stage I to 39.7% in clinical stage III. The decisive change of the prognosis is between the clinical stages IIb and IIc. An adjuvant cytostatic therapy should, if performed, be aggressive. Relapses in the clinical stage I are possible and call for a critical valuation of the primary stage. A retarded cytostatic polychemotherapy should be performed only under certain conditions.

Antineoplastic Combined Chemotherapy Protocols↗
PubMed1987

[Localized fibrous mesothelioma of the pleura. Morphological studies of 7 cases].

Localized tumors of pleura should clearly be separated from diffuse pleural mesotheliomas due to clinical and pathological peculiarities. But there is some dispute on their proper interpretation and hence disagreement about their correct designation. Some call them "fibrous tumors (fibromas)" of pleura and appreciate by this term the fibroplastic potency of tumor cells and the lacking relation to overlying mesothelial surface cells, others prefer the name "fibrous mesothelioma" and allude to an at least abortive mesothelial differentiation within the tumor tissue. Examining 7 own tumor cases by means of light microscopic, topo- and immunohistochemical as well as electron microscopic methods the investigations were aimed at analysing the morphological structure of these tumors and subjecting it to a critical reappraisal with regard to a suited designation. Histologically, a marked variability of histological structure and texture was found, there were regions with similarities to fibromas, fibrous histiocytomas, hemangiopericytomas and nerve sheath tumors as well as blastema-like areas. Occasionally a vasoformative activity could be seen. In three tumors a few epithelioid cell complexes were visible, but neither keratin intermediate filaments nor UEA-I reactivity was to be demonstrated by immunohistochemistry. Antibodies against factor VIII associated antigen only marked endothelial cells of vascular structures. By the electron microscopic examination of 1 case abortive mesothelial differentiations were visualized (some lumen-like space formations, stub-like cellular projections of the cavity facing side, primitive intercellular junctions and fragmented basal lamina-like material). The findings inclusive the immunohistochemical results are discussed and jointly taken for sustaining the interpretation of these tumors as mesotheliomas and for militating against the classification as simple pleural fibromas or fibrous pleural tumors, respectively.

Adult↗