Biomedical subjects

D K Chopin

Publications and source records attributed to D K Chopin.

85 records · Page 5Linked to original sources

PubMed1993

[Surgical anatomy of the lymphatic drainage of the bladder].

Descriptive anatomy teaches us that the lymphatic drainage of the bladder converges directly on to the middle and internal chains of the external iliac nodes, but never on to the external chain, sometimes on a sacral common iliac node and widely on internal iliac nodes. Surgical observations confirm this: no lymph node metastasis in the external chain of external iliac nodes is noticed, nor common iliac adenopathy without upstream invasion. On the basis of these descriptions, lymphadenectomy was performed from inguinal ligament to iliac bifurcation. Anatomical data largely explain pelvic recurrences due to the existence of unremoved common iliac nodes or unrecognized hypogastric nodes because of difficult access without sacrificing efferent branches of internal iliac artery.

Humans↗

PubMed1992

Immunohistochemical detection of acidic fibroblast growth factor in bladder transitional cell carcinoma.

Acidic fibroblast growth factor (aFGF) is a regulatory peptide which, on account of its structural homologies with the products of oncogenes, is involved in cell proliferation, differentiation, and motility. We previously reported the presence of aFGF in the urine of patients with transitional cell carcinoma (TCC). aFGF can also induce the motility of a rat-derived bladder carcinoma cell line (NBTII). This immunohistochemical study used polyclonal rabbit antibodies against acidic and basic FGF and peroxidase detection. Native NBTII nude mice xenografts and aFGF transfected NBTII (NFS14) nude mice xenografts were used as tissue controls for antibody specificity. The samples included 4 normal urothelia and 12 TCC. In addition, cytospins of 4 different tumoral cell lines of human bladder and normal bladder cells were stained. The results showed strong immunostaining in all tumoral urothelium samples using anti-aFGF and a very low amount of staining or none at all in healthy tissues. A primary analysis suggested that the strongest reaction was obtained in high-grade tumors (3 + vs + for lower-grade tumors). Using bFGF antibody, strong immunohistochemical staining was detected on basal membranes and stromal vessels and none in urothelium. These data confirm aFGF expression in the epithelial cell compartment of bladder cancer and the likely involvement of this regulatory peptide in the biology of TCC.

Animals↗

PubMed1992

Fast magnetic resonance imaging in the evaluation of tumoral obstructions of the inferior vena cava.

A prospective study was conducted on 9 patients with suspected obstruction of the inferior vena cava on ultrasonography, dynamic computerized tomography and/or inferior venacavography during the course of a retroperitonal tumor to assess the value of magnetic resonance imaging with gradient echo sequences. The findings on gradient echo magnetic resonance imaging were compared with the other imaging modalities and surgical or necropsy results. Our results suggest that gradient echo magnetic resonance imaging is the most appropriate imaging modality to diagnose and delineate the extent of inferior vena caval obstruction.

Adult↗

PubMed1990

Immunohistochemical markers defined by monoclonal antibodies and response to bacillus Calmette-Guérin endovesical immunotherapy for superficial bladder tumors.

As bacillus Calmette-Guérin (BCG) immunotherapy is highly effective for most but not all superficial bladder tumors, there is a need to define predictors of response to this mode of treatment. We have investigated a panel of markers defined by monoclonal antibodies, directed against tumor-associated transitional cell carcinoma antigen (G4 and E7), epidermal growth factor receptor, cytokeratin (CK) 18 and blood group antigens A, B and H, using an indirect immunoperoxidase staining on paraffin sections. Twenty superficial bladder tumors (T1) treated with intravesical BCG therapy (10 responders and 10 nonresponders) were tested with this panel. Among the responders, expression of CK18 antigen was positive in 7 and negative in 3, whereas in the nonresponder group it was positive in 2 and negative in 8. The difference was statistically significant (p less than 0.05). Loss of expression of CK18 antigenicity was associated with recurrence or progression of superficial bladder tumors following BCG therapy, indicating that changes in CK patterns should be investigated as potential predictive markers for response to BCG.

Administration, Intravesical↗

PubMed1990

[Cystic cancer of the kidney].

Four cases of cystic renal adenocarcinoma in adults free of renal dysfunction are reported. The authors propose a diagnostic approach for atypical cysts that takes the new imaging techniques into account (ultrasonography and CT scan initially, MRI subsequently). A practical therapeutic strategy is also described: because intraoperative histologic diagnosis is difficult partial nephrectomy to allow an intraoperative study of the entire cyst is advocated; extensive nephrectomy, which is the only procedure that ensures total exeresis of the carcinoma, is performed either immediately if the intraoperative study of the cyst or of its aseptic necrotic and hemorrhagic content is positive, or later if subsequent histologic studies reveal a malignancy.

Adenocarcinoma↗

PubMed1989

Conservative treatment of renal allograft rupture with polyglactin 910 mesh and gelatin resorcin formaldehyde glue.

Renal-sparing treatment of spontaneous renal allograft rupture remains a surgical challenge, since profuse hemorrhage may result from these friable kidneys during surgical repair. A technique is proposed to achieve control of local bleeding with a synthetic glue (gelatin, resorcin and formaldehyde) and external compression with a polyglactin 910 absorbable mesh. We report 4 cases of spontaneous allograft rupture associated with rejection and bleeding was controlled in all 4. Three grafts were preserved with more than 1 year of followup. The other graft had to be removed for uncontrolled vascular rejection despite satisfactory control of renal fractures. Renal wrapping with external compression is proposed to improve results of conservative management of renal allograft rupture.

Adult↗

PubMed1989

Use of vena cava to obtain additional length for the right renal vein during transplantation of cadaveric kidneys.

During cadaveric organ harvesting removal of the right renal vein and a segment of the vena cava may facilitate venous anastomosis of the right kidney. The technique to obtain adequate length of the right renal vein using the vena cava is simple, physiological and effective. A segment of the inferior vena cava should be harvested with the right but not necessarily with the left kidney. The use of a long segment of vena cava makes the renal transplantation much easier and does not interfere with multiorgan procurement. The method is most useful when the right renal vein is extremely short or when the recipient has a large abdomen and a deep pelvis.

Anastomosis, Surgical↗

PubMed1988

[Use of the vena cava in renal transplantation. Extension of the right renal vein].

Vena cava is a providential material for the transplant surgeon. Prolongation of the right renal vein using vena cava is a simple and effective procedure. Consequently the inferior vena cava must be harvested with the right and not the left kidney for which it is unnecessary. The use of a long segment of vena cava makes the renal transplantation and easy, well-defined procedure without interfering with the requirements of multiorgan donation.

Humans↗

PubMed1988

[Contribution of monoclonal antibodies within the framework of urothelial tumors of the bladder].

The advent of hybridoma technology has proved a major breakthrough in the field of tumor immunology and enables analysis at the molecular level of tumor heterogeneity. This approach seems particularly promising in urothelial tumors of the bladder and carries implications for diagnosis, prognosis and therapy of this disease. Two murine monoclonal antibodies (G4 and E7), obtained by xenogenic immunization of Balb/c mice with a human transitional carcinoma cell line (TCC) (647V) have proved especially useful. Use of a panel of monoclonal antibodies covering the entire spectrum of TCCs, most of which are already available, can be expected to result in significant modifications in the management of urothelial tumors. Advances in the field of monoclonal antibody technology may provide specific tools applicable to humans in vivo.

Antibodies, Monoclonal↗

PubMed1986

Detection of transitional cell carcinoma in bladder by intravesical injection of monoclonal antibodies.

Monoclonal antibodies produced against tumor associated antigens of human bladder transitional cell carcinoma have proved to be useful in detecting malignant cells in tumor sections and bladder washings. The present study evaluated the capacity of one such antibody G4 to identify sites of malignancy when introduced into the lumen of intact bladders immediately after cystectomy. An ex vivo immunoperoxidase staining (IPS) method was applied to four cystectomy specimens--three with solitary invasive transitional cell carcinomas (TCC) and one with carcinoma in situ (CIS) using monoclonal antibody G4 injected intravesically immediately after cystectomy. The tumor sites, normal appearing mucosa and other exposed and non exposed tissues from the same patient were examined for G4 binding. In all cases preferential intravesical binding of G4 antibody to the tumor size was demonstrated without binding to the normal mucosa except in one case. This study showed that monoclonal antibody injected intravesically can be focused on the tumor site and indicates that G4 and other antibodies may be useful intravesically for selectively assessing field changes associated with malignancies or as specific therapeutic agents.

Antibodies, Monoclonal↗

PubMed1986

[Place of prostatectomy and radiotherapy in the treatment of cancer of the prostate confined to the prostate gland (T0, T1, T2)].

The most appropriate treatment for localized prostatic cancer remains controversial: which tumors should be treated? And what is the respective place of radical prostatectomy versus radiotherapy. The results obtained with these two treatment modalities reported by various american centers are compared for their ability to control local disease. In the absence of any decisive therapeutic trial, the data suggest that radical prostatectomy represents a valuable therapeutic approach in selected cases.

Humans↗

PubMed1985

Monoclonal antibodies against transitional cell carcinoma for detection of malignant urothelial cells in bladder washing.

The diagnosis of transitional cell carcinoma by cytological examination of exfoliated urinary cells is important in the early detection and followup of patients with this disease. Proper interpretation requires a skilled pathologist. Accuracy also is influenced by collection methods and nonmalignant pathological conditions of the bladder. An immunocytochemical technique using monoclonal antibodies G4 and E7 successfully identified tumor-associated antigens on the surface of transitional carcinoma cells obtained by bladder washings. The method, which uses immunoperoxidase staining, was compared to conventional Papanicolaou staining of bladder washings from 75 patients with and without transitional cell carcinoma. Patients were divided into 4 groups: group 1 (nontumor control)--15 patients with no pathological condition of the bladder or nonmalignant urological diseases, group 2 (nontransitional cell carcinoma)--19 patients with other urological malignancies, group 3-18 patients with active transitional cell carcinoma and group 4-23 patients with a history of transitional cell carcinoma but no evidence of tumor at the time of the washing. The incidence of positive staining in these groups was 0, 5, 78 and 0 per cent, respectively. The diagnostic value of immunoperoxidase staining was similar to that of Papanicolaou staining in the control group and in patients with high grade transitional cell carcinoma, and provided specific morphological criteria not possible by conventional cytology studies. Interpretation of immunoperoxidase staining was difficult in washings with a large number of inflammatory cells if endogenous peroxidase activity was not blocked properly. The application of the immunoperoxidase staining method for diagnosis of low grade tumor is under further investigation.

Antibodies, Monoclonal↗

PubMedDate not supplied

Polyphenols purified from the Brazilian aroeira plant (Schinus terebinthifolius, Raddi) induce apoptotic and autophagic cell death of DU145 cells.

Polyphenols extracted from many plants have shown antiproliferative and antitumor activities in a wide range of carcinogenesis models. The antiproliferative effects of polyphenols purified from the Brazilian aroeira plant (Schinus terebinthifolius, Raddi) were investigated on the androgen-insensitive DU145 human prostatic carcinoma cell line. A F3 fraction purified from leaf extract inhibited the DU145 cell proliferation more than 30-fold compared to the crude extract. By flow cytometric analysis, the polyphenol fraction was demonstrated to induce G0/G1 cell growth arrest and cell apoptosis. This apoptosis was evidenced by caspase 3 stimulation in F3-treated cells as compared to crude extract treated cells. The acid phosphatase activity of lysosomes was strongly activated in the lysosomal fraction of the F3-treated DU145 cells. This lysosomal activation, together with the appearance of autophagic vacuoles, suggests that "type 2 physiological cell death" was also involved in this antiproliferative effect. HPLC analysis of this F3 fraction showed 18 different subfractions. Among these subfractions, F3-3, F3-7 and F3-13 strongly inhibited DU145 cell proliferation in a dose-dependent manner. However, the nature of these polyphenols remains unknown since only one (Isoquercitrin) of the tested pure polyphenols co-migrated with F3-13. Since lysosomotropic drugs are considered as possible regulators of lysosome activity, aroeira polyphenols could target lysosomes of prostatic cancer cells to induce autophagic cell death.

Anacardiaceae↗