The role of nonsteroidal regulators in control of oocyte and follicular maturation.
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Biomedical subjects
Publications and source records attributed to D J Hoover.
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Ethanol was administered to rats by oral intubation 3 h after intraperitoneal administration of calcium carbimide. Increased blood acetaldehyde concentration and the presence of acetaldehyde in brain were found for the calcium carbimide--ethanol interaction. There was no detectable brain acetaldehyde for ethanol administration following saline treatment. The magnitude of the acetaldehyde concentration in blood and brain during the calcium carbimide--ethanol interaction was directly dependent on the doses of calcium carbimide (0.7, 3.5, 7.0 mg/kg) and ethanol (0.5, 1.0, 1.5 g/kg). There was an exponential relationship between brain acetaldehyde concentration and blood acetaldehyde concentration. The threshold blood acetaldehyde concentration, above which acetaldehyde was found in brain, was approximately 6 micrograms/mL.
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A gas-liquid chromatographic procedure, involving head-space analysis, is described for the determination of ethanol and acetaldehyde in the blood, liver, heart, and brain of the rat. The lower limit of sensitivity of the assay is 0.05 mg/ml (mg/g) ethanol and 0.25 micrograms/ml (micrograms/g) acetaldehyde. The coefficient of variation of the method is less than 6% for ethanol and less than 9% for acetaldehyde. This procedure has been used in the investigation of the calcium carbimide-ethanol interaction.
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