Studies on the mechanism of replication of adenovirus DNA. III. Electron microscopy of replicating DNA.
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Adenoviridae↗
Biomedical subjects
D J Ellens
Publications and source records attributed to D J Ellens.
Mechanism of formation of bacteriophage phiX174 circular and catenated dimer RF+ DNA.
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Bromodeoxyuridine↗
A mutant of Escherichia coli K12 deficient in the 5'-3' exonucleolytic activity of DNA polymerase I. II. Purification and properties of the mutant enzyme.
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Coliphages↗
Studies on the mechanism of replication of adenovirus DNA. II. The nature of single-stranded DNA in replicative intermediates.
Replicative intermediates of adenovirus type 5 DNA contain large stretches of single-stranded DNA. We have shown that this single-stranded DNA is mainly of parental origin, whereas all new DNA synthesized during one round of replication has a double-stranded structure. Hybridization experiments of the single-stranded DNA with isolated complementary strands of adenovirus type 5 DNA showed that this DNA hybridized only with the viral L-strand (the strand with the lower equilibrium density in alkaline CsCl) indicating that it represents the viral H-strand. This observation implies that replication always starts from one and the same molecular end. Electron microscopy of partially denatured Y-shaped intermediates confirmed this and showed that replication started from the molecular right end (the end richest in A-T base pairs). In conclusion, we have shown that replication of adenovirus type 5 DNA starts at the molecular right end, displacing the parental H-strand.
Adenosine Triphosphate↗
Linear intermediates in the replication of adenovirus DNA.
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Adenosine Triphosphate↗