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Biomedical subjects

D J Alexander

Publications and source records attributed to D J Alexander.

At least 181 records · Page 10Linked to original sources
PubMed1975

The ribonucleic acid of infectious bronchitis virus.

Analysis of the nucleic acid of infectious bronchitis virus by SDS polyacrylamide gel electrophoresis revealed an RNA of molecular weight 9.0 times 10-6 Daltons. The RNA was shown to have a sedimentation coefficient of 50.

Bronchitis↗
PubMed1975

Effect of pH on the growth and cytopathogenicity of avian infectious bronchitis virus in chick kidney cells.

The growth of avian infectious bronchitis virus (IBV) in chick kidney cells at different pH values in the range 6.0-9.0 demonstrated that although the virus was released at a much faster rate at the higher pH values the titre tended to drop more quickly. At the acid pH values the virus was released more slowly but reached a maximum titre similar to that at the higher pH values and showed only minimum reduction in infectivity up to 49 hours post inoculation. The stability of virus in tissue culture medium was shown to be directly related to pH 6.0-8.0, being more stable at the acid pH values. The degree of cytopathogenicity induced in chick kidney cells following infection with IBV was directly related to the pH at which the cells were incubated, occurring earlier and more extensively in cells at the higher pH values. Cell macromolecule synthesis in chick kidney cells was inhibited following infection with IBV and was apparently due to cell damage and death.

Animals↗
PubMed1975

Infectious bursal disease agent: morphology by negative stain electron microscopy.

The virus of infectious bursal disease of chickens was studied by immune electron microscopy. Negatively-stained preparations revealed morphological similarities with both the bluetongue virus group, and the virus of infectious pancreatic necrosis of trout. Results indicated that the small particle found in such preparations is a degradation product of the large particle.

Centrifugation, Density Gradient↗
PubMed1975

Preliminary characterisation of paramyxovirus isolated from a parrot.

A virus, designated 0121, which was isolated from a parrot, was shown to be a paramyxovirus, which was serologically related to the paramyxoviruses Bangor/flinch/N. Ireland/73 (Bangor) and Yucaipa/chicken/California/60(PMY). However, the 0121 virus differed in several properties from both the PMY and the Bangor viruses. The virus was not pathogenic for chickens. I was designated Paramyxovirus: Parrot/England/0121/74.

Animals↗