Biomedical subjects

D I Dzhumalieva

Publications and source records attributed to D I Dzhumalieva.

3 recordsLinked to original sources

PubMedDate not supplied

[Experimental hepatitis E infection in piglets].

A sample of patient's faeces containing virus-like particles (VLP) of 27-34 nm was obtained during an outbreak of hepatitis E in the Kirghiz SSR. The identity of the VLP to hepatitis E virus and etiological association with the disease were demonstrated by immune electron microscopy and infection of Macaca fascicularis monkeys. This isolate of hepatitis E virus is able to induce experimental infection in domestic piglets which was very similar to experimental hepatitis E in primates when infected orally, intravenously and by the combined routes. The clinical manifestations included acute biochemical and histological hepatitis, excretion of hepatitis E virus in faeces, icteric sclerae and skin, hepatitis virus presence in the material from mesenteric lymph nodes. Immunosuppression aggravated hepatitis E infection in piglets. Piglet-to-piglet transmission of hepatitis E virus was demonstrated. During passages of the virus in piglets a shortening of the incubation period and the absence of jaundice was observed.

Animals↗

PubMedDate not supplied

[Further study of experimental hepatitis E in piglets].

Experimental infection of piglets with a fecal pool containing hepatitis E virus (HEV) strains Osh-25 and Osh-228 confirmed previous results obtained with HEV strain Osh-205 on susceptibility of these animals to hepatitis E. A biphasic increase of transaminase activities, histopathologic changes in the liver, virus excretion with feces were observed in the animals. The genome of viral particles in piglet feces conformed, at least partially, to human HEV RNA. Ultrastructural abnormalities of piglet hepatocytes resembled those in primates with experimental hepatitis E. The number of positive results (during follow-up of the particles and HEV RNA in various organs of experimental animals) was the highest on day 10 after infection. Both the particles and HEV RNA were present in the lymphoid tissue over the entire follow-up period, from day 5 to day 15 postinfection.

Animals↗

PubMedDate not supplied

[The possible involvement of rodents in the spread of viral hepatitis E].

The outbreak of hepatitis E among humans in July-October 1989 in the village of Dzhigdele (140 km to the northeast of Osh) is described. During this outbreak 23 rodents of different species (4 Turkestan rats, 15 house mice and 4 wood mice) were caught within a radial distance of 1 km from the village. 5 out of these 23 animals were found to have hepatitis E virus, detected by the method of immuno-electron microscopy, in their blood sera. Under experimental conditions 7 noninbred white mice were inoculated with material obtained from a previously infected Javanese macaque and a hepatitis E patient. The use of immunoelectron microscopy made it possible to reveal the excretion of the virus with feces on days 4 to 19-23 after inoculation and the development of specific seroconversion in all 7 infected animals. On the basis of these data a suggestion was made that rodents probably played a certain role in the process of the spread of viral hepatitis E.

Acute Disease↗